Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,285,635	G→T	intergenic (+126055/+135302)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,285,635	0	G	T	100.0%	174.8 / NA	65	intergenic (+126055/+135302)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/34);  total (31/34)

CAATTGTCTGGCTTTTTCATTCATTTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACGTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGTTAAAAAACATTATCTTTTACAAC  >  1/1285398‑1285873                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               cAATTGTCTGGCTTTTTCATTCATTTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAGTGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGtcatca                                                                                                                                                                                                                                   >  1:449815/1‑251 (MQ=255)   aTTGTCTGGCTTTTTCATTCATTTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGt                                                                                                                                                                                                                                 <  1:551362/251‑1 (MQ=255)          ggCTTTTTCATTCATTTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCACAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGa                                                                                                                                                                                                                          >  1:431451/1‑251 (MQ=255)          ggCTTTTTCATTCATTTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGa                                                                                                                                                                                                                          >  1:887294/1‑251 (MQ=255)                      cattTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAg                                                                                                                                                                                                              >  1:607137/1‑251 (MQ=255)                               gATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACgg                                                                                                                                                                                                     >  1:859552/1‑251 (MQ=255)                                aTAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACgg                                                                                                                                                                                                     <  1:558441/250‑1 (MQ=255)                                        tAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGt                                                                                                                                                                                             <  1:775476/250‑1 (MQ=255)                                        tAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGt                                                                                                                                                                                             <  1:305027/250‑1 (MQ=255)                                         aaGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGc                                                                                                                                                                                           <  1:533284/251‑1 (MQ=255)                                            cGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCAc                                                                                                                                                                                         <  1:264567/250‑1 (MQ=255)                                             gtgtCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCg                                                                                                                                                                                       >  1:231967/1‑251 (MQ=255)                                              tgtCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGt                                                                                                                                                                                             <  1:537781/244‑1 (MQ=255)                                                     ccGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGt                                                                                                                                                                                             <  1:57858/237‑1 (MQ=255)                                                     ccGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCaa                                                                                                                                                                               <  1:861525/251‑1 (MQ=255)                                                         cggAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATAc                                                                                                                                                                            <  1:294630/250‑1 (MQ=255)                                                               gggACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGCAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCAc                                                                                                                                                                                         >  1:434111/1‑231 (MQ=255)                                                                ggACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGa                                                                                                                                                                                                            >  1:543906/1‑211 (MQ=255)                                                                ggACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTttatta                                                                                                                                                                     <  1:782208/250‑1 (MQ=255)                                                                ggACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTtattat                                                                                                                                                                    >  1:894083/1‑251 (MQ=255)                                                                      ggCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGc                                                                                                                                                                                           <  1:763637/222‑1 (MQ=255)                                                                                 cATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATacac                                                                                                                                                    <  1:805663/250‑1 (MQ=255)                                                                                    aaTTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTg                                                                                                                                                                                                                                         >  1:236720/1‑162 (MQ=255)                                                                                    aaTTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAAt                                                                                                                                                           <  1:537914/240‑1 (MQ=255)                                                                                    aaTTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACcaca                                                                                                                                                >  1:174468/1‑251 (MQ=255)                                                                                        aTCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAg                                                                                                                                            <  1:621649/251‑1 (MQ=255)                                                                                          cGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          <  1:3030/251‑1 (MQ=255)                                                                                          cGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          <  1:463199/251‑1 (MQ=255)                                                                                          cGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          <  1:857049/251‑1 (MQ=255)                                                                                          cGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          <  1:314913/251‑1 (MQ=255)                                                                                               tGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTgcgc                                                                                                                                     <  1:82575/251‑1 (MQ=255)                                                                                                gCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTg                                                                                                                                                                                                                                         >  1:745824/1‑150 (MQ=255)                                                                                                gCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          >  1:648760/1‑245 (MQ=255)                                                                                                       catcatTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          >  1:646641/1‑238 (MQ=255)                                                                                                                   cAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAAc                                                                                                                  <  1:766271/250‑1 (MQ=255)                                                                                                                     aTTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAg                                                                                                                                            <  1:509346/222‑1 (MQ=255)                                                                                                                       tCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAAt                                                                                                             <  1:310003/251‑1 (MQ=255)                                                                                                                                           atatTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGt                                                                                                                                                                                             >  1:507725/1‑151 (MQ=255)                                                                                                                                            tatTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTtattat                                                                                                                                                                    <  1:221909/175‑1 (MQ=255)                                                                                                                                            tatTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACAttttt                                                                                        >  1:869805/1‑251 (MQ=255)                                                                                                                                                  ggCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAAt                                                                                                             >  1:356224/1‑224 (MQ=255)                                                                                                                                                   gCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACAt                                                                                  <  1:674568/250‑1 (MQ=255)                                                                                                                                                             gtgcgtgcCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACg                                                                                                                                                                                                      >  1:259417/1‑124 (MQ=255)                                                                                                                                                                   gcCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGaaa                                                                  >  1:20970/1‑250 (MQ=255)                                                                                                                                                                    ccTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          <  1:213804/177‑1 (MQ=255)                                                                                                                                                                    ccTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAAcag                                                                                                                          >  1:829556/1‑193 (MQ=255)                                                                                                                                                                    ccTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACAt                                                                                  >  1:414860/1‑233 (MQ=255)                                                                                                                                                                    ccTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGc                                                                             <  1:655237/238‑1 (MQ=255)                                                                                                                                                                     cTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGAc                                                                                                                                          >  1:499720/1‑176 (MQ=255)                                                                                                                                                                           tGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTg                                                         <  1:441622/251‑1 (MQ=255)                                                                                                                                                                              aGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACAt                                                                                  >  1:3761/1‑223 (MQ=255)                                                                                                                                                                               gTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTg                                                         <  1:858012/247‑1 (MQ=255)                                                                                                                                                                                            tcaGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAAt                                                                                                                                                           >  1:322288/1‑136 (MQ=255)                                                                                                                                                                                               gTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAggg                                                                              >  1:462851/1‑210 (MQ=255)                                                                                                                                                                                                   tGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGtttcttt                                  >  1:588262/1‑250 (MQ=255)                                                                                                                                                                                                      aaGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGc                                                                             >  1:705329/1‑204 (MQ=255)                                                                                                                                                                                                           gTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGt                                                                                                                                                                                             <  1:838636/87‑1 (MQ=255)                                                                                                                                                                                                           gTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGt                         >  1:766048/1‑251 (MQ=255)                                                                                                                                                                                                           gTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGt                         >  1:507981/1‑251 (MQ=255)                                                                                                                                                                                                             ataGCGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTttat                                                                                                                                                                       <  1:473470/107‑1 (MQ=255)                                                                                                                                                                                                                 cGGGGGTTTTTGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGTTaaaaa                   <  1:131346/251‑1 (MQ=255)                                                                                                                                                                                                                       tttttGTTGTGTTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGa                                                                    >  1:603568/1‑196 (MQ=255)                                                                                                                                                                                                                                tgtTCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGTTAAAAAACATTATCTTTTAc     <  1:210340/250‑1 (MQ=255)                                                                                                                                                                                                                                 gtTCCAGAAATACTTTTGCTGTCATCAGTTTGGAGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGTTAAAAAACATTATCTTTTACaa   >  1:460167/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttCCAGAAATACTTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGTTAAAAAACATTATCTTTTACAAc  <  1:858307/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               CAATTGTCTGGCTTTTTCATTCATTTTGCCGATAAATTGTAAGCGTGTCATACCGGCGGAGCGGGACAAGGCGTGAGCGGCATAATTATCGGAATGCACCAACATCATTTTCAGCAATTCACGGCAAGAAATCGGCGTATATTTAGGCAATTTGGTGTGCGTGCCTTTAATGTAGTCAAAATCATCATCAGTAATGGAAGCAGTATAGCGGGGGTTTTTGTTGTGTTCCAGAAATACGTTTGCTGTCATCAGTTTGGTGACGGAAGCGATAGGACGCACGGTATTCGGTGCACCGCTTTCCAATACTTTATTATGGGTAAAATCATACACCACATAAGACTGCGCTATGGCAACAGCAGGCAACAGAATCAACAAAGGAAAAACATTTTTTAACATAGGGCATTCAGGGAAACTAAAACTGTTGCTATTTTAGCAGGTTTCTTTCGACAGGGTTAAAAAACATTATCTTTTACAAC  >  1/1285398‑1285873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑