Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,284,953	A→C	intergenic (+125373/+135984)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,284,953	0	A	C	100.0%	157.7 / NA	55	intergenic (+125373/+135984)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/38);  total (17/38)

GATGTTTCAAGCATTAAGGGATCTCACAATGCATTATTTAAACAAAAAATTGCCTAGATTATACAGGATTTTGATCGTTCTAACAGCTTGAAAATAGCAAGTGTGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTAATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAGGCACTTTATTGACGAACACCAGATTGTAGCC  >  1/1284721‑1285151                                                                                                                                                                                                                                         |                                                                                                                                                                                                       gattgtttgtgatgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCAt                                                                                                                                                                                       <  1:88677‑M2/169‑1 (MQ=255)  attgtttgtgatgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATAc                                                                                                                                                                                     <  1:524373‑M2/171‑1 (MQ=255)     gtttgtgatgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATc                                                                                                                                                                                                     <  1:842372‑M2/155‑1 (MQ=255)     gtttgtgatgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTg                                                                                                                                                                                   >  1:118732‑M2/78‑250 (MQ=255)      tttgtgatgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGc                                                                                                                                                                                  <  1:881119‑M2/174‑1 (MQ=255)       ttgtgatgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCat                                                                                                                                                                                <  1:403880‑M2/176‑1 (MQ=255)            atgtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATatcat                                                                                                                                                                           <  1:696314‑M2/181‑1 (MQ=255)              gtttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGc                                                                                                                                                                                  <  1:336355‑M2/174‑1 (MQ=255)               tttcaagcattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATcc                                                                                                                                                                         <  1:250902‑M2/183‑1 (MQ=255)                      cattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAGCAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAAGCGGCGAAAAAAGGGGCACAAAATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGt                                                                                                                                                                  <  1:178034‑M2/190‑1 (MQ=255)                      cattaagggatctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATatc                                                                                                                                                                             <  1:694877‑M2/179‑1 (MQ=255)                               atctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCgcagca                                                                                                                                                         >  1:228714‑M2/52‑250 (MQ=255)                                tctcacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATcc                                                                                                                                                                         >  1:420310‑M2/51‑233 (MQ=255)                                   cacaatgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTg                                                                                                                                                    >  1:97078‑M2/48‑251 (MQ=255)                                       atgcattatttaaacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTg                                                                                                                                                    >  1:66186‑M2/44‑247 (MQ=255)                                             tatttaaaaaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAAcg                                                                                                                                          <  1:798363‑M2/214‑1 (MQ=255)                                                   aacaaaaaattgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTAc                                                                                                                                    <  1:485400‑M2/220‑1 (MQ=255)                                                            ttgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATAtt                                                                                                                           <  1:376611‑M2/229‑1 (MQ=255)                                                             tgcctagattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATAc                                                                                                                                                                                     >  1:258933‑M2/22‑192 (MQ=255)                                                                  agattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTcatcat                                                                                                                                                                                                   <  1:694215‑M2/157‑1 (MQ=255)                                                                  agattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATAc                                                                                                                                                                                     <  1:196339‑M2/171‑1 (MQ=255)                                                                  agattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCa                                                                                                                     >  1:148538‑M2/17‑251 (MQ=255)                                                                  aaattatacagaatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAAAATTTATAACAGTTTTCTTTTCAAGATACCGATTCTATAAAAATCGGCAAAAAAAGGGGCACAAGATCCATTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTcattc                                                                                                                                                                                     <  1:549650‑M2/171‑3 (MQ=255)                                                                   gattatacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCaa                                                                                                                    >  1:483043‑M2/16‑251 (MQ=255)                                                                     ttatacaggatttAACAGCTTGAAAGTAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTc                                                                                                                                                                                         <  1:92080‑M2/167‑1 (MQ=255)                                                                       atacaggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAACCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGt                                                                                                                                                                  <  1:801315‑M2/190‑1 (MQ=255)                                                                            ggatttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTAttttt                                                                                                           >  1:164557‑M2/7‑251 (MQ=255)                                                                              atttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAAc                                                                                                                                 >  1:368494‑M2/5‑227 (MQ=255)                                                                              atttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTgg                                                                                                         >  1:269542‑M2/5‑251 (MQ=255)                                                                              atttAACAGCTTGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTgg                                                                                                         >  1:593525‑M2/5‑251 (MQ=255)                                                                                         tGAAAATAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAg                                                                                              <  1:653629/251‑1 (MQ=255)                                                                                              aTAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGata                                                                                         >  1:452668/1‑251 (MQ=255)                                                                                               tAGCAAGTGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAatttattt                                                                                                             <  1:889852/230‑1 (MQ=255)                                                                                                aGCAAGGGCGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCAc                                                                                                                                                        <  1:130105/186‑1 (MQ=255)                                                                                                      tgcggtgAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCgaag                                                                                 <  1:339160/248‑1 (MQ=255)                                                                                                        cggtgAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCgaaga                                                                                <  1:858421/249‑1 (MQ=255)                                                                                                          gtgAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAAc                                                                              <  1:681469/250‑1 (MQ=255)                                                                                                                 tttGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACTTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTc                                                                      <  1:162995/251‑1 (MQ=255)                                                                                                                    gaagaaTTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTAtttt                                                                                                            <  1:361135/210‑1 (MQ=255)                                                                                                                     aagaaTTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCTGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGc                                                                  <  1:449800/251‑1 (MQ=255)                                                                                                                         aTTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGc                                                                                                                                                                                                >  1:845522/1‑121 (MQ=255)                                                                                                                         aTTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATatca                                                                                                                                                                            <  1:563234/141‑1 (MQ=255)                                                                                                                         aTTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCa                                                                                                                               >  1:768040/1‑186 (MQ=255)                                                                                                                         aTTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTc                                                                      <  1:29500/243‑1 (MQ=255)                                                                                                                                                    aCGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCa                                   <  1:60039/251‑1 (MQ=255)                                                                                                                                                       tttCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTGGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAgg                                <  1:481359/250‑1 (MQ=255)                                                                                                                                                          cTATAAAAATCGGCGAAAAAAGGGGCACAAGACCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCa                                   <  1:114481/245‑1 (MQ=255)                                                                                                                                                               aaaaTCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGACTATTGCAAGGCACTTTAt                        <  1:144755/251‑1 (MQ=255)                                                                                                                                                               aaaaTCGGCGAAAAAAGGGGCACAAGACCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAGGCACTTTAt                        <  1:862168/251‑1 (MQ=255)                                                                                                                                                                                  gCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAGGCACTTTATTGACGAACACCAGATTGt      <  1:408681/250‑1 (MQ=255)                                                                                                                                                                                     caAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGc                                                                                      <  1:247759/167‑1 (MQ=255)                                                                                                                                                                                     caAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCa                                                            >  1:406121/1‑193 (MQ=255)                                                                                                                                                                                     caAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAGGCACTTTATTGACGAACACCAGATTGTAGcc  <  1:757478/251‑1 (MQ=255)                                                                                                                                                                                            ccTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCa                                                            <  1:815469/186‑1 (MQ=255)                                                                                                                                                                                             cTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAg                                                                                                                                                                   <  1:292111/82‑1 (MQ=255)                                                                                                                                                                                                             tttCGGATTAATTCGTCCATTCCGGCTTCATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAGGCACTTTATTGACGAACACCAg           >  1:405315/1‑218 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                       GATGTTTCAAGCATTAAGGGATCTCACAATGCATTATTTAAACAAAAAATTGCCTAGATTATACAGGATTTTGATCGTTCTAACAGCTTGAAAATAGCAAGTGTGGTGAAAATTTGAAGAATTTATAACGGTTTTCTTTTCAAGATAACGATTCTATAAAAATCGGCGAAAAAAGGGGCACAAGATCCCTTCTAGCCCGCCACTTTTCGGATTAATTCGTCCATTCCGGCTTAATCATAGCCCTCTTCATACTGCATATCATCCACAAAGTCTCGCAGCACCTTGCTGTTTAACGCGGTACAACCATATTTTTCCAATTTATTTTTGGTGAAACTGGAGCGATATGCCGAAGAACTGTTATTCAGGCTAATCACGCCGATGGTGGCACGATTATTGCAAGGCACTTTATTGACGAACACCAGATTGTAGCC  >  1/1284721‑1285151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑