Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,282,757	A→G	intergenic (+123177/+138180)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,282,757	0	A	G	100.0%	159.4 / NA	57	intergenic (+123177/+138180)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

TATCGGCGCGAACTAATTTCATGTTTTCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGACAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCATAGCGAATGA  >  1/1282521‑1282994                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              tATCGGCGCGAACTAATTTCATGTTTTCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAAt                                                                                                                                                                                                                                 <  1:78909/251‑1 (MQ=255)    cGGCGCGAACTAATTTCATGTTTTCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATaaa                                                                                                                                                                                                                              <  1:375697/251‑1 (MQ=255)           aaCTAATTTCATGTTTTCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCa                                                                                                                                                                                                                       >  1:681584/1‑251 (MQ=255)                  cagaTGTTTTCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGt                                                                                                                                                                                                                >  1:550105/4‑251 (MQ=255)                        ttttCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGc                                                                                                                                                                                                          <  1:88297/251‑1 (MQ=255)                        ttttCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGc                                                                                                                                                                                                          <  1:187424/251‑1 (MQ=255)                        ttttCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGc                                                                                                                                                                                                          <  1:593310/251‑1 (MQ=255)                            ctctTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAAc                                                                                                                                                                                                                                      <  1:603905/219‑1 (MQ=255)                            ctctTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAAt                                                                                                                                                                                                                                 >  1:299907/1‑224 (MQ=255)                            ctctTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTGCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAAt                                                                                                                                                                                                                            <  1:443637/229‑1 (MQ=255)                              ctTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGat                                                                                                                                                                                                    <  1:183093/251‑1 (MQ=255)                              ctTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGat                                                                                                                                                                                                    >  1:363823/1‑251 (MQ=255)                                 aaGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAgg                                                                                                                                                                                                           >  1:227123/1‑241 (MQ=255)                                    tAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATAc                                                                                                                                                                                               <  1:413490/250‑1 (MQ=255)                                    tAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATAc                                                                                                                                                                                               <  1:350029/250‑1 (MQ=255)                                    tAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATAc                                                                                                                                                                                               <  1:810352/250‑1 (MQ=255)                                     aGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGc                                                                                                                                                                                                          >  1:379446/1‑238 (MQ=255)                                      gCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATAc                                                                                                                                                                                               <  1:486458/248‑1 (MQ=255)                                       cTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCg                                                                                                                                                                                             <  1:190992/249‑1 (MQ=255)                                       cTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCg                                                                                                                                                                                             <  1:389777/249‑1 (MQ=255)                                                            ctgctgAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCg                                                                                                                                                                       >  1:400471/1‑250 (MQ=255)                                                                          tttttCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGAtt                                                                                                                                                        <  1:798661/251‑1 (MQ=255)                                                                              tcttcACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGAt                                                                                                                                                    <  1:203992/251‑1 (MQ=255)                                                                              tcttcACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGAt                                                                                                                                                    <  1:346208/251‑1 (MQ=255)                                                                                    cTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATcc                                                                                                                                               >  1:190152/1‑250 (MQ=255)                                                                                           ccGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATAttt                                                                                                                                        <  1:712862/250‑1 (MQ=255)                                                                                              gTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACAATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCg                                                                                                                                    >  1:445410/1‑251 (MQ=255)                                                                                                  aaaaTAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCatat                                                                                                                                          >  1:863841/1‑241 (MQ=255)                                                                                                   aaaTAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGa                                                                                                                                                                                                                                        >  1:588818/1‑146 (MQ=255)                                                                                                          gCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGAc                                                                                                                         >  1:820222/1‑250 (MQ=255)                                                                                                             aTCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATa                                                                                                                                                   <  1:4382/221‑1 (MQ=255)                                                                                                                       gAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGAt                                                                                                                                                    >  1:129405/1‑210 (MQ=255)                                                                                                                                   aaaTGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAgg                                                                                               <  1:662660/251‑1 (MQ=255)                                                                                                                                        cgcTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAAc                                                                                          <  1:291047/251‑1 (MQ=255)                                                                                                                                                       ttAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAaag                                                                                   >  1:277543/1‑243 (MQ=255)                                                                                                                                                       ttAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCa                                                                           >  1:295309/1‑251 (MQ=255)                                                                                                                                                            aaaCCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAAt                                                                        <  1:785491/249‑1 (MQ=255)                                                                                                                                                                    cacaTAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGaaaa                                                              >  1:284700/1‑251 (MQ=255)                                                                                                                                                                      caTAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGc                                                                                                                                     >  1:285728/1‑178 (MQ=255)                                                                                                                                                                      caTAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCAc                                                                                                                    <  1:505871/195‑1 (MQ=255)                                                                                                                                                                      caTAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGaa                                                                                     <  1:758405/226‑1 (MQ=255)                                                                                                                                                                       aTAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGt                                                           <  1:265035/251‑1 (MQ=255)                                                                                                                                                                               tGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAgg                                                   >  1:802442/1‑251 (MQ=255)                                                                                                                                                                                  aaaGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATAc                                                                                                                                                                                               >  1:34116/1‑108 (MQ=255)                                                                                                                                                                                     gTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAAt                                              >  1:720015/1‑250 (MQ=255)                                                                                                                                                                                                      ttgcttgcCACATAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAacac                            <  1:235050/251‑1 (MQ=255)                                                                                                                                                                                                               acaTAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGat                                                                                                                                                                                                    >  1:820966/1‑74 (MQ=255)                                                                                                                                                                                                                caTAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGAc                                                                                                                         >  1:842938/1‑148 (MQ=255)                                                                                                                                                                                                                caTAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTa                  >  1:220951/1‑251 (MQ=255)                                                                                                                                                                                                                caTAAATGGCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTa                  >  1:426532/1‑251 (MQ=255)                                                                                                                                                                                                                        gCTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCATa          >  1:288995/1‑251 (MQ=255)                                                                                                                                                                                                                         cTTCAAAAAGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCATAg         >  1:817828/1‑251 (MQ=255)                                                                                                                                                                                                                                 aGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAg                                                                                                                                                           <  1:297767/97‑1 (MQ=255)                                                                                                                                                                                                                                 aGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTTAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCATAGCGAATGa  >  1:586238/1‑250 (MQ=255)                                                                                                                                                                                                                                 aGATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCAt           >  1:233744/1‑241 (MQ=255)                                                                                                                                                                                                                                  gATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCCAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCa            >  1:430749/1‑239 (MQ=255)                                                                                                                                                                                                                                  gATAAGAGAAGGCAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCATAGCGa      >  1:611619/1‑245 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              TATCGGCGCGAACTAATTTCATGTTTTCTCTTAAGTAGCTTAAAGAAAAATATTTTTTGCTGCTGAAATGTGTTTTTTCTTCACTTTCTGCCGGTGGAAAATAAGGCAATCTAAAGGCGAAAATGACTTCAAATGCGCTGAAAATCAACATTAAGAAACCGATCACATAAATACTGGAAAGTATATCCGCCGGGTTGTTGCTTGCCACATAAATGGCTTCAAAAAGATAAGAGAAGACAAAGGAACTGAATAAAATCGCCACGATGGTTAAGGCTTGGATAATACCGTTGGCGGCGCCCAGATTTTCCGTACCCACAATAGATTTGATAATCCCATATTTGGCGGGAGAATAGACCGCACTTTGCGCCGCCAGAATCAAGGTCAACAGGAAAGCAAGCTCAAATGCACCGAAAAAGTAGCTGAGGAAAATCGCAAAGGAAATTAACACGCTGGCAGTAGCGGCATAGCGAATGA  >  1/1282521‑1282994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑