Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,281,458	A→G	intergenic (+121878/+139479)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,281,458	0	A	G	100.0%	222.1 / NA	78	intergenic (+121878/+139479)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/40);  total (38/40)

GTTGTTCAGGGTTTGTTTAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCAGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCTTCCGGGAAAAGCGCCACCACTTCGCCATTTT  >  1/1281220‑1281689                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        gttgttCAGGGTTTGTTTAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATg                                                                                                                                                                                                                             >  1:127497/1‑251 (MQ=255)    gttCAGGGTTTGTTTAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCa                                                                                                                                                                                                                          >  1:160136/1‑251 (MQ=255)          ggTTTGTTTAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGAt                                                                                                                                                                                                                      <  1:460467/249‑1 (MQ=255)             ttgtttAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGt                                                                                                                                                                                                                 <  1:102693/251‑1 (MQ=255)              tgtttAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGt                                                                                                                                                                                                                 >  1:678107/1‑250 (MQ=255)               gtttAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCa                                                                                                                                                                                                               >  1:175990/1‑251 (MQ=255)                       aGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGCTAATGTCACGTTTAc                                                                                                                                                                                                        >  1:19299/1‑250 (MQ=255)                        gTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACcg                                                                                                                                                                                                      >  1:433535/1‑251 (MQ=255)                         tttGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACcg                                                                                                                                                                                                      <  1:817686/250‑1 (MQ=255)                                  aaCCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTgg                                                                                                                                                                                            >  1:361942/1‑251 (MQ=255)                                   aCCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGt                                                                                                                                                                                           <  1:57252/251‑1 (MQ=255)                                       acacAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCa                                                                                                                                                                                                               >  1:877846/1‑227 (MQ=255)                                       acacAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGt                                                                                                                                                                                       >  1:779817/1‑251 (MQ=255)                                              aCTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTtat                                                                                                                                                                                <  1:118940/251‑1 (MQ=255)                                              aCTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTtat                                                                                                                                                                                <  1:227856/251‑1 (MQ=255)                                              aCTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTtat                                                                                                                                                                                <  1:540735/251‑1 (MQ=255)                                                                ggTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTtat                                                                                                                                                                                >  1:475325/1‑233 (MQ=255)                                                                ggTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACggg                                                                                                                                                              >  1:169116/1‑251 (MQ=255)                                                                ggTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACggg                                                                                                                                                              >  1:759266/1‑251 (MQ=255)                                                                ggTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACggg                                                                                                                                                              >  1:214215/1‑251 (MQ=255)                                                                ggTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACggg                                                                                                                                                              >  1:738404/1‑251 (MQ=255)                                                                ggTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGCTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACggg                                                                                                                                                              >  1:582379/1‑251 (MQ=255)                                                                 gTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGga                                                                                                                                                             >  1:738585/1‑251 (MQ=255)                                                                  ttAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCTCGGgag                                                                                                                                                            <  1:842108/251‑1 (MQ=255)                                                                     aTTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAg                                                                                                                                                         <  1:10849/251‑1 (MQ=255)                                                                           tCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGttt                                                                                                                                                                                                          <  1:155660/196‑1 (MQ=255)                                                                           tCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTc                                                                                                                                                                      <  1:709468/232‑1 (MQ=255)                                                                           tCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTACCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCt                                                                                                                                                       >  1:441126/1‑247 (MQ=255)                                                                            ccccATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTtat                                                                                                                                                                                >  1:522697/1‑221 (MQ=255)                                                                             cccATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAAc                                                                                                                                                  >  1:424729/1‑250 (MQ=255)                                                                                  gCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGt                                                                                                                                                                                                                 >  1:877759/1‑182 (MQ=255)                                                                                    tGTCGGCAACACATTCGCGGAATAAATTGC‑TTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAAc                                                                                                                                         <  1:556423/251‑1 (MQ=255)                                                                                     gTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCtt                                                                                                                                                                                              >  1:579190/1‑198 (MQ=255)                                                                                     gTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATaaa                                                                                                                                          >  1:263900/1‑250 (MQ=255)                                                                                      tCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAAc                                                                                                                                         <  1:771663/250‑1 (MQ=255)                                                                                      tCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAAc                                                                                                                                         <  1:178123/250‑1 (MQ=255)                                                                                      tCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAAc                                                                                                                                         <  1:87748/250‑1 (MQ=255)                                                                                                   cgcgGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAAc                                                                                                                           <  1:510470/251‑1 (MQ=255)                                                                                                        aaTAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGcac                                                                                                                      <  1:811397/251‑1 (MQ=255)                                                                                                                  tttttGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACg                                                                                                            <  1:255023/251‑1 (MQ=255)                                                                                                                  tttttGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACg                                                                                                            <  1:199524/251‑1 (MQ=255)                                                                                                                    tttGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTc                                                                                                          <  1:253839/251‑1 (MQ=255)                                                                                                                           tttCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACg                                                                                                            >  1:120113/1‑242 (MQ=255)                                                                                                                              ccAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTGCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCActct                                                                                                <  1:447210/251‑1 (MQ=255)                                                                                                                                 gCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATg                                                                                                                                                                            >  1:670818/1‑172 (MQ=255)                                                                                                                                 gCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTa                                                                                             >  1:517367/1‑251 (MQ=255)                                                                                                                                   cAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAAt                                                                                           <  1:268821/251‑1 (MQ=255)                                                                                                                                   cAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAAt                                                                                           <  1:89019/251‑1 (MQ=255)                                                                                                                                    aaGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATa                                                                                          >  1:635921/1‑251 (MQ=255)                                                                                                                                       aaTAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATAc                                                                                         <  1:98987/249‑1 (MQ=255)                                                                                                                                       aaTAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATAc                                                                                         <  1:631334/249‑1 (MQ=255)                                                                                                                                       aaTAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATAc                                                                                         <  1:153547/249‑1 (MQ=255)                                                                                                                                           ggTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTAc                                                                                                                                                                                                        >  1:235978/1‑134 (MQ=255)                                                                                                                                           ggTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAAtt                                                                                   >  1:537414/1‑251 (MQ=255)                                                                                                                                            gTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCaaa                                                                                                                                 >  1:885392/1‑204 (MQ=255)                                                                                                                                             tCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCTCGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACTCACACGTCTTGCCACTCTTTAATACTTAATTc                                                                                  <  1:63417/250‑1 (MQ=255)                                                                                                                                             tCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTc                                                                                  <  1:50609/250‑1 (MQ=255)                                                                                                                                                    cTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCAc                                                                          <  1:132931/251‑1 (MQ=255)                                                                                                                                                             ggAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAAt                                                                  <  1:170381/250‑1 (MQ=255)                                                                                                                                                              gAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAAtt                                                                 <  1:166045/250‑1 (MQ=255)                                                                                                                                                               aaaTAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCg                                                               >  1:789160/1‑251 (MQ=255)                                                                                                                                                                                gaagaGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTtata                                              >  1:420432/1‑251 (MQ=255)                                                                                                                                                                                   gagaAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAg                                             >  1:98241/1‑249 (MQ=255)                                                                                                                                                                                    agaAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGc                                            <  1:96175/249‑1 (MQ=255)                                                                                                                                                                                    agaAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCt                                           <  1:350399/250‑1 (MQ=255)                                                                                                                                                                                      aaaGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTg                                          <  1:627085/249‑1 (MQ=255)                                                                                                                                                                                            aaCTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGccc                                  <  1:321348/251‑1 (MQ=255)                                                                                                                                                                                            aaCTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGccc                                  <  1:556346/251‑1 (MQ=255)                                                                                                                                                                                              cTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAAc                                                                                                                                         >  1:401922/1‑146 (MQ=255)                                                                                                                                                                                              cTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGcc                                                                            <  1:513789/207‑1 (MQ=255)                                                                                                                                                                                              cTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCtt                                >  1:453537/1‑251 (MQ=255)                                                                                                                                                                                                ccaccaCTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTtat                                                                                                                                                                                <  1:845083/105‑1 (MQ=255)                                                                                                                                                                                                ccaccaCTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGc                                                                                                                 >  1:167775/1‑168 (MQ=255)                                                                                                                                                                                                                 aTGGCAACATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCTTCCGGGAAAAGCGCcaccac             <  1:761891/251‑1 (MQ=255)                                                                                                                                                                                                                        cATGATCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCTTCCGGGAAAAGCGCCACCACTTCGCCa      >  1:386641/1‑251 (MQ=255)                                                                                                                                                                                                                             tCGATAAACTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCTTCCGGGAAAAGCGCCACCACTTCGCCAtttt  >  1:552847/1‑250 (MQ=255)                                                                                                                                                                                                                                    aCTCACGAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCTTCCGGGAAAAg                      <  1:618472/223‑1 (MQ=255)                                                                                                                                                                                                                                          gAATCGGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGc                                            >  1:657510/1‑195 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        GTTGTTCAGGGTTTGTTTAAACAGTTTGCTGAAAACCAACACAGCACTGATAAATTTTGCATTGGTTAATTCCGTCCCCATGCTGTCGGCAACACATTCGCGGAATAAATTGCTTTTTGCCATTTCCAGCCAAGAATAGGTCAACGGCTGTTGACGGGAAATAAAGCTTTCCCAAGAAGAGAAAGATAACTCCACCACTTTTTGTTTCATGGCAACATGATCGATAAACTCACGAATCAGTTTGCCGAATGCCACGATAATGTCACGTTTACCGCGTTGCTTGGTGAGTTCTTTATAATGCTCGTCCGCACGGGAGAAGCTGGAACCCCATAAACCACGCAAATAAAACGGCACAACGCACACGTCTTGCCACTCTTTAATACTTAATTCGTAGCCACGTTGAAATTCGTTAATTTGTCCGTTATAGCTGATATGCCCTTCCGGGAAAAGCGCCACCACTTCGCCATTTT  >  1/1281220‑1281689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑