Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,276,451	A→G	intergenic (+116871/+144486)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,276,451	0	A	G	100.0%	196.4 / NA	68	intergenic (+116871/+144486)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/30);  total (38/30)

TCGGCTAAATCCAGCTGTTCGCGGCATTCTTCAATGCGATCGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATATAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTCCTGTGCGGAAAGTACGCTATCATCAAA  >  1/1276207‑1276694                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    tCGGCTAAATCCAGCTGTTCGCGGCATTCTTCAATGCGATCGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAAcc                                                                                                                                                                                                                                               <  1:765862/251‑1 (MQ=255)    gCTAAATCCAGCTGTTCGCGGCATTCTTCAATGCGATCGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGGATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGa                                                                                                                                                                                                                                             >  1:282186/1‑250 (MQ=255)    gCTAAATCCAGCTGTTCGCGGCATTCTTCAATGCGATCGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAAcc                                                                                                                                                                                                                                               <  1:773915/248‑1 (MQ=255)                                cAATGCGATCGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGc                                                                                                                                                                                                                <  1:177497/251‑1 (MQ=255)                                         cGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCAtttt                                                                                                                                                                                                       >  1:429432/1‑251 (MQ=255)                                           gttgaaCTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTg                                                                                                                                                                                                     >  1:462366/5‑251 (MQ=255)                                           gTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTg                                                                                                                                                                                                     >  1:543895/1‑251 (MQ=255)                                               aaCTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACg                                                                                                                                                                                                 <  1:791751/251‑1 (MQ=255)                                                      aTCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAg                                                                                                                                                                                          <  1:774790/251‑1 (MQ=255)                                                           ccAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACaaa                                                                                                                                                                                                                                         <  1:719718/199‑1 (MQ=255)                                                                     cAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAAt                                                                                                                                                                           >  1:555312/1‑251 (MQ=255)                                                                         tGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTc                                                                                                                                                                       <  1:359639/251‑1 (MQ=255)                                                                             gAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGc                                                                                                                                                                    <  1:207396/250‑1 (MQ=255)                                                                               attaattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGc                                                                                                                                                                                                                >  1:262819/1‑204 (MQ=255)                                                                                 taattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTa                                                                                                                                                                                                                     >  1:132592/1‑197 (MQ=255)                                                                                  aattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAAc                                                                                                                                                                                                                                                <  1:690933/169‑1 (MQ=255)                                                                                  aattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTg                                                                                                                                                                                                     >  1:741945/1‑212 (MQ=255)                                                                                  aattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAg                                                                                                                                                                                          <  1:241866/223‑1 (MQ=255)                                                                                  aattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACtt                                                                                                                                                               >  1:203796/1‑250 (MQ=255)                                                                                  aattTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTc                                                                                                                                                              <  1:626821/251‑1 (MQ=255)                                                                                       aTTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAAcc                                                                                                                                                                                                                                               >  1:858778/1‑165 (MQ=255)                                                                                               acTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGcc                                                                                                                                                 <  1:164147/251‑1 (MQ=255)                                                                                                cTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGc                                                                                                                                                  >  1:185170/1‑249 (MQ=255)                                                                                                cTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTAATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGc                                                                                                                                                  >  1:490610/1‑249 (MQ=255)                                                                                                           cTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCAc                                                                                                                                                         <  1:704367/231‑1 (MQ=255)                                                                                                                     atcCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGttttt                                                                                                                           >  1:214658/1‑251 (MQ=255)                                                                                                                                   gAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCGAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGa                                                                                                             >  1:440340/1‑251 (MQ=255)                                                                                                                                    aaTTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGAc                                                                                                            <  1:154533/251‑1 (MQ=255)                                                                                                                                                        ggAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCa                                                                                                                                                                  <  1:237042/177‑1 (MQ=255)                                                                                                                                                               gTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCCCTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAg                                                                                 >  1:424573/1‑251 (MQ=255)                                                                                                                                                                  tAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAAt                                                                              <  1:601688/251‑1 (MQ=255)                                                                                                                                                                             cAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCa                                                                   >  1:272251/1‑251 (MQ=255)                                                                                                                                                                                          ctgctgATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCa                                                                                                                <  1:857840/193‑1 (MQ=255)                                                                                                                                                                                              tgATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCa                                                                                                                                                                  >  1:141523/1‑139 (MQ=255)                                                                                                                                                                                               gATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAaac                                                                                                       <  1:874132/197‑1 (MQ=255)                                                                                                                                                                                                 ttGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGcaccac                                               <  1:365435/251‑1 (MQ=255)                                                                                                                                                                                                 ttGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGcaccac                                               <  1:645783/251‑1 (MQ=255)                                                                                                                                                                                                   ggTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACaa                                             >  1:243760/1‑251 (MQ=255)                                                                                                                                                                                                    gTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAg                                                                                                                                                                                          >  1:633782/1‑109 (MQ=255)                                                                                                                                                                                                    gTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAAt                                                                              <  1:573826/217‑1 (MQ=255)                                                                                                                                                                                                    gTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACaa                                             >  1:709043/1‑250 (MQ=255)                                                                                                                                                                                                         aGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACg                                                                                                                       >  1:569920/1‑171 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTTAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:648311/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACg                                                                                                                                                        >  1:685436/1‑127 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACg                                                                                                                                                        <  1:312453/127‑1 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGcc                                                                                                                                                 >  1:519931/1‑134 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGc                                                                         >  1:776376/1‑206 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAAtt                              >  1:676314/1‑249 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:120174/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:197658/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             <  1:190120/250‑1 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:711711/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:695131/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:678686/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTc                             >  1:560987/1‑250 (MQ=255)                                                                                                                                                                                                                     ttCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTcc                            <  1:257943/250‑1 (MQ=255)                                                                                                                                                                                                                     ttCTGGATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTcc                            <  1:96709/250‑1 (MQ=255)                                                                                                                                                                                                                         ggATTATCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTCCtgtg                        >  1:389674/1‑250 (MQ=255)                                                                                                                                                                                                                              aTCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAg                                                                                                                                                                                          <  1:4470/83‑1 (MQ=255)                                                                                                                                                                                                                              aTCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGAt                                                               <  1:17809/206‑1 (MQ=255)                                                                                                                                                                                                                              aTCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGccc                                        >  1:618559/1‑229 (MQ=255)                                                                                                                                                                                                                              aTCTTGGAACGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTCCTGTGCGGaaa                  >  1:728608/1‑251 (MQ=255)                                                                                                                                                                                                                                       cGCTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTc                                                                                                                                                                       >  1:607493/1‑93 (MQ=255)                                                                                                                                                                                                                                         cTAATGCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGAt                                                               >  1:646349/1‑195 (MQ=255)                                                                                                                                                                                                                                              gCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGt                                                                                                                                                                         <  1:359454/84‑1 (MQ=255)                                                                                                                                                                                                                                              gCTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTCCTGTGCGGAAAGTACGCTat         >  1:95811/1‑244 (MQ=255)                                                                                                                                                                                                                                               cTAAATGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTCCTGTGCGGAAAGTACGCTATCATCaaa  <  1:188/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aaaTGTAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACg                                                                 <  1:709550/185‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TCGGCTAAATCCAGCTGTTCGCGGCATTCTTCAATGCGATCGGTTAAACTGTCATCCGCCAATAATGGCAATTGCGGAATTAATTTATTCAACAACTGCATACGTTCTTTCGCATCATCCAAACGAATGCGAATTTGTTGTTCGCCGGTCAGGAATTGGTTTAGTTCGCGTTCAATTTCATTGCGCTGCTGATTGGTCCGAGCCATGACCTGTTCTGGATTATCTTGGAACGCTAATGCTAAATATAAACCGACAAATTGGCTGAAATGTTCGTGTAAACGCTGGCATTTTTGCACGTCAAAAGCGCGTTGAGCGTAATGTTCCGCCACTTCCTCACGTTTTGCCTGTAATTCTTCCAAATGTTTTTCACGAGCGGCACGACCAAACAACGGAATTTCCGGGAATTTAGAATAACGCAATTCACGATCGGACACTTGCACCACAACGCCCAGTTCCAATTCCTGTGCGGAAAGTACGCTATCATCAAA  >  1/1276207‑1276694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑