Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,275,208	A→G	intergenic (+115628/+145729)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,275,208	0	A	G	100.0%	212.1 / NA	74	intergenic (+115628/+145729)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/33);  total (41/33)

GATATAATTTCGCAATAGCCTCGGAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTACCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGCATCGCGCAGATATTCGTTATCGGCAA  >  1/1274981‑1275448                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 gATATAATTTCGCAATAGCCTCGGAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGc                                                                                                                                                                                                                            >  1:705290/1‑250 (MQ=255)  atatAATTTCGCAATAGCCTCGGAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCagag                                                                                                                                                                                                                                    >  1:307387/1‑241 (MQ=255)  atatAATTTCGCAATAGCCTCGGAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCa                                                                                                                                                                                                                          >  1:238846/1‑251 (MQ=255)     taATTTCGCAATAGCCTCGGAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGtt                                                                                                                                                                                                                       <  1:178159/251‑1 (MQ=255)                        gAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTaa                                                                                                                                                                                                    >  1:682760/1‑251 (MQ=255)                          aaaaGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAAct                                                                                                                                                                                                  <  1:478890/251‑1 (MQ=255)                                   aCGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTa                                                                                                                                                                                         <  1:615323/251‑1 (MQ=255)                                      aTTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGc                                                                                                                                                                                      <  1:860072/251‑1 (MQ=255)                                      aTTGTCGGTAAATAAATCCTGATAATCCGATTGGCTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAg                                                                                                                                                                                       >  1:572020/1‑250 (MQ=255)                                             gTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAg                                                                                                                                                                               >  1:710788/1‑251 (MQ=255)                                               aaataaatCCTGATAATCCGATTGGTTGCCGGAAAGCACATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGCTTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAg                                                                                                                                                                               >  1:581966/1‑249 (MQ=255)                                                aataaatCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTc                                                                                                                                                                            <  1:144856/251‑1 (MQ=255)                                                     atCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGAttt                                                                                                                                                                       >  1:494935/1‑251 (MQ=255)                                                       ccTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCagag                                                                                                                                                                                                                                    >  1:726180/1‑188 (MQ=255)                                                        cTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCa                                                                                                                                                                    >  1:308597/1‑251 (MQ=255)                                                          gATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCAtt                                                                                                                                                                  >  1:18738/1‑251 (MQ=255)                                                              aTCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTg                                                                                                                                                                >  1:396399/1‑249 (MQ=255)                                                               tCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTc                                                                                                                                                             <  1:209258/251‑1 (MQ=255)                                                                            cGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATc                                                                                                                                                <  1:536514/251‑1 (MQ=255)                                                                                           acaacaTGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGt                                                                                                                                                                                           <  1:376405/193‑1 (MQ=255)                                                                                            caacaTGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCa                                                                                                                                >  1:75114/1‑251 (MQ=255)                                                                                             aacaTGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAg                                                                                                                               >  1:733894/1‑251 (MQ=255)                                                                                             aacaTGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAg                                                                                                                               >  1:709697/1‑251 (MQ=255)                                                                                                          tATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACgg                                                                                                                  >  1:277272/1‑251 (MQ=255)                                                                                                           aTCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGa                                                                                                                              >  1:480975/1‑238 (MQ=255)                                                                                                             cACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATa                                                                                                               >  1:279615/1‑251 (MQ=255)                                                                                                             cACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATa                                                                                                               >  1:100139/1‑251 (MQ=255)                                                                                                                 aaTATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGaa                                                                                                                                                                                 <  1:19220/181‑1 (MQ=255)                                                                                                                 aaTATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGAttt                                                                                                                                                                       >  1:900392/1‑191 (MQ=255)                                                                                                                  atatTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGaa                                                                                                                             <  1:512856/232‑1 (MQ=255)                                                                                                                  atatTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGtt                                                                                                           >  1:106154/1‑250 (MQ=255)                                                                                                                    atTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTcg                                                                                                         >  1:95875/1‑250 (MQ=255)                                                                                                                           accaAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATg                                                                                                 >  1:898268/1‑251 (MQ=255)                                                                                                                             caAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACg                                                                                                                   <  1:747125/231‑1 (MQ=255)                                                                                                                                   cACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGtat                                                                                          <  1:149287/250‑1 (MQ=255)                                                                                                                                   cACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGtat                                                                                          <  1:213866/250‑1 (MQ=255)                                                                                                                                   cACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGtat                                                                                          <  1:814214/250‑1 (MQ=255)                                                                                                                                       gATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGTGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCg                                                                                      >  1:609808/1‑250 (MQ=255)                                                                                                                                       gATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACg                                                                                                                   >  1:647711/1‑221 (MQ=255)                                                                                                                                       gATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCg                                                                                      >  1:761191/1‑250 (MQ=255)                                                                                                                                       gATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCg                                                                                      >  1:468803/1‑250 (MQ=255)                                                                                                                                        aTTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGc                                                                                     <  1:738730/250‑1 (MQ=255)                                                                                                                                            cACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAat                                                                                                                            >  1:482166/1‑207 (MQ=255)                                                                                                                                              ccTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGtt                                                                                                           <  1:823711/222‑1 (MQ=255)                                                                                                                                                 gACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTa                                                                                                                                                                                         <  1:576628/141‑1 (MQ=255)                                                                                                                                                 gACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGCCGTCGGCTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGaa                                                                            >  1:298048/1‑250 (MQ=255)                                                                                                                                                        ggCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGAt                                                                                                  <  1:229852/221‑1 (MQ=255)                                                                                                                                                            atatTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTAt                                                                 <  1:575113/250‑1 (MQ=255)                                                                                                                                                               ttttGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTc                                                             <  1:215298/251‑1 (MQ=255)                                                                                                                                                                        ccTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTcc                                                            <  1:138072/243‑1 (MQ=255)                                                                                                                                                                        ccTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCg                                                    >  1:846771/1‑251 (MQ=255)                                                                                                                                                                          ttGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTAt                                                                 >  1:670063/1‑236 (MQ=255)                                                                                                                                                                                  gCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAg                                                                                                    >  1:386087/1‑193 (MQ=255)                                                                                                                                                                                  gCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGGTGTTgg                                                                                             >  1:503669/1‑200 (MQ=255)                                                                                                                                                                                  gCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATAc                                                                                        <  1:477526/205‑1 (MQ=255)                                                                                                                                                                                  gCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTc                                           >  1:341541/1‑250 (MQ=255)                                                                                                                                                                                  gCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACACTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTAt                                                                 <  1:25188/228‑1 (MQ=255)                                                                                                                                                                                  gCATACGAATACGATTTTGTTCACGTTGAATGGCTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGCAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTc                                           >  1:598290/1‑250 (MQ=255)                                                                                                                                                                                   cATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTc                                           <  1:172503/249‑1 (MQ=255)                                                                                                                                                                                    atacgaatacgaTTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGa                                        <  1:771724/251‑1 (MQ=255)                                                                                                                                                                                               aTTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATg                                                                                                 <  1:496781/183‑1 (MQ=255)                                                                                                                                                                                               aTTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAgg                             >  1:235393/1‑251 (MQ=255)                                                                                                                                                                                                ttttGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGc                            <  1:877311/251‑1 (MQ=255)                                                                                                                                                                                                ttttGTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGc                            <  1:92520/251‑1 (MQ=255)                                                                                                                                                                                                    gTTCACGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGa                                                                             <  1:111074/198‑1 (MQ=255)                                                                                                                                                                                                         cGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAg                                                                              >  1:861514/1‑192 (MQ=255)                                                                                                                                                                                                         cGTTGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAAc                                                                           <  1:521743/195‑1 (MQ=255)                                                                                                                                                                                                           ttGAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTA‑TTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGCATCGCGCAGata                <  1:555447/251‑1 (MQ=255)                                                                                                                                                                                                             gAATGGTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGAt                                                                                                  <  1:63596/168‑1 (MQ=255)                                                                                                                                                                                                                  gTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACg                                  >  1:572935/1‑227 (MQ=255)                                                                                                                                                                                                                  gTTTTACGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGCATCGCGCAGata                <  1:655343/245‑1 (MQ=255)                                                                                                                                                                                                                        cGCATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGCATCGCGCAGATATTCGTTATCgg     >  1:481219/1‑250 (MQ=255)                                                                                                                                                                                                                          cATAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGCATCGCGCAGATATTCGTTATCGGCaa  >  1:300290/1‑251 (MQ=255)                                                                                                                                                                                                                           aTAATGTTTGCCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAgg                             >  1:368507/1‑223 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 GATATAATTTCGCAATAGCCTCGGAAAAAGTCATACGATTGTCGGTAAATAAATCCTGATAATCCGATTGGTTGCCGGAAAGCGCATCCAACAACATGGCGTGCGTATCACGAATATTCACCACCAAACGCACTGATTTCACCTGACCGAAGGCGATATTTTGCAAGCCTTGATTCAGCATACGAATACGATTTTGTTCACGTTGAATGGTTTTACGCATAATGTTTACCACGCTTTCAGAGCTGATCGCCAGTTTCTGTTCACGACCGGTTAACTCTTCCGTTAAGCGGGAAAGCTCGATTTCCATTTGCTCGATGGCATCAATCGGGTCGTCGGTTTTCAGAATATCCTGACGGATACGTTCGCGTAGATGTTGGTATACCGCAATAAAGAACCGCACTTTATTTTCCGGTTTGCGACTGTCTTCCGATAAACGTAAGGCATCGCGCAGATATTCGTTATCGGCAA  >  1/1274981‑1275448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑