Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,273,713 G→A intergenic (+114133/+147224) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,273,7130GA96.5% 71.5 / ‑5.9 29intergenic (+114133/+147224)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/15);  minor base C (1/0);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTTTTGCCATCATTTTTCTCAAATCTAAGCCAAAATTTTATCTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCGGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATGAGAACGCGATAAACAAGGTTGTTGGGAATGCGCCGCCGCTTTTCGCTACTTGTT  >  1/1273540‑1273933
                                                                                                                                                                             |                                                                                                                                                                                                                            
cGTTTTTTGCCATCATTTTTCTCAAATCTAAGCCAAAATTTTATCTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGcac                                                                                                                                                 <  1:188027/251‑1 (MQ=255)
                   tctcAAATCTAAGCCAAAATTTTATCTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGtt                                                                                                                                                                                                               <  1:377419/170‑1 (MQ=255)
                                      ttttATCTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTcc                                                                                                           <  1:891907/251‑1 (MQ=255)
                                          aTCTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCAttt                                                                                                       <  1:365962/251‑1 (MQ=255)
                                            cTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACtt                                                                                                                                                                                             >  1:636576/1‑163 (MQ=255)
                                            cTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTgg                                                                                                     >  1:178295/1‑251 (MQ=255)
                                             taataaTACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGgt                                                                                                    >  1:401330/1‑251 (MQ=255)
                                              aataatACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTcc                                                                                                           >  1:124662/1‑243 (MQ=255)
                                                                 tGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCaaataaa                                                                                <  1:713406/251‑1 (MQ=255)
                                                                          gCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGctctc                                                                                                                   >  1:552761/1‑207 (MQ=255)
                                                                             aaaTACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCCGAAAAAACCAACGTTGGATTAGAGTCAAAACTTTGATGCGCTTTAATg                                                                                                                                                                              >  1:252738/1‑145 (MQ=255)
                                                                             aaaTACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGc                                                                                                                       <  1:37092/200‑1 (MQ=255)
                                                                               atacaatacCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATg                                                                                                                                                                              <  1:376803/143‑1 (MQ=255)
                                                                               atacaatacCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGc                                                                                                                                                   >  1:238491/1‑170 (MQ=255)
                                                                               atacaatacCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAaata                                                                                                                                       >  1:227842/1‑182 (MQ=255)
                                                                               atacaatacCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACaa                                                                                                                          <  1:888451/195‑1 (MQ=255)
                                                                               atacaatacCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTc                                                                                                            <  1:275701/209‑1 (MQ=255)
                                                                                tacaatacCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAAc                                                                           <  1:84249/241‑1 (MQ=255)
                                                                                         tAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATg                                                        <  1:413799/251‑1 (MQ=255)
                                                                                              aattaattTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATGAGaa                                                    >  1:396958/1‑250 (MQ=255)
                                                                                                       tGGTTATTTTCTCTTTAAAAATTATTGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGt                                                                                                                                                                                                                >  1:730485/1‑85 (MQ=255)
                                                                                                            aTTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGt                                                                                                                                                                             <  1:28651/115‑1 (MQ=255)
                                                                                                            aTTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATGAGAACGCGATAAACAAGgt                                     <  1:647461/251‑1 (MQ=255)
                                                                                                                                    ccGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTc                                                                                                            >  1:346206/1‑156 (MQ=255)
                                                                                                                                             gTGCCTAAACAAATTACACCGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATGAGAACGCGATAAACAAGGTTGTTGGGAATGCGCCGCCGCTTTTCGCTACtt     <  1:180299/250‑1 (MQ=255)
                                                                                                                                                       aaaTTACACTGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCttc                                                                                                                                                                                  >  1:34812/1‑66 (MQ=255)
                                                                                                                                                               ccGATATTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATAtt                                                                                                                                                                >  1:734411/1‑77 (MQ=255)
                                                                                                                                                                  atatTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATGAGAACGCGATAAACAAGGTTGTTGGGAATGCGCCGCCGCTTTTCGCTACTTGtt  >  1:220424/1‑232 (MQ=255)
                                                                                                                                                                    atTCATACCAGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTaa                                                                                            <  1:299529/140‑1 (MQ=255)
                                                                                                                                                                             |                                                                                                                                                                                                                            
CGTTTTTTGCCATCATTTTTCTCAAATCTAAGCCAAAATTTTATCTAATAATACAAACAAAAGATTGCCGTTAAGCTAAATACAATACCTAACCAATTAATTTTGGTTATTTTCTCTTTAAAAATTAATGCGCCGGTGAAGGTGCCTAAACAAATTACACCGATATTCATACCGGCAAAAACCAACGTTGGATTAGAGCCAAAACTTTGATGCGCTTTAATGTAAAACAAAATATTAAAGAAATTCAGCACACCTAAAATAATGCCGCCTACAAAGCTCTCTATATTCCATTTGGTTTGTTTAAACAGCAAATAAATGAACATAATGCAGGCTGCCAATGAGAACGCGATAAACAAGGTTGTTGGGAATGCGCCGCCGCTTTTCGCTACTTGTT  >  1/1273540‑1273933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑