Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,272,598	A→C	intergenic (+113018/+148339)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,272,598	0	A	C	100.0%	152.6 / NA	57	intergenic (+113018/+148339)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/27);  total (30/27)

TCTTGAAAACTAAGATTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGAGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTAAAGGTGCAATTAACGTGGTATTTCCGC  >  1/1272357‑1272843                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      tCTTGAAAACTAAGATTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTgg                                                                                                                                                                                                                                              <  1:283756/251‑1 (MQ=255)          cTAAGATTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGt                                                                                                                                                                                                                                     >  1:126288/1‑251 (MQ=255)             aGATTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGtttt                                                                                                                                                                                                                                  >  1:87628/1‑251 (MQ=255)              gATTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAACCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTa                                                                                                                                                                                                                                 >  1:897161/1‑251 (MQ=255)               aTTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAg                                                                                                                                                                                                                                >  1:646614/1‑251 (MQ=255)               aTTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAg                                                                                                                                                                                                                                >  1:853433/1‑251 (MQ=255)                              tttttCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCg                                                                                                                                                                                                                 <  1:221929/251‑1 (MQ=255)                                     ggTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACAttt                                                                                                                                                                                                          >  1:358512/1‑251 (MQ=255)                                           aaaCTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGat                                                                                                                                                                                                    <  1:637672/251‑1 (MQ=255)                                           aaaCTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGat                                                                                                                                                                                                    <  1:348438/251‑1 (MQ=255)                                                 cGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATatgaat                                                                                                                                                                                              <  1:217765/251‑1 (MQ=255)                                                   tAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATatgaatg                                                                                                                                                                                             <  1:205390/250‑1 (MQ=255)                                                            cATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATg                                                                                                                                                                                   <  1:857010/251‑1 (MQ=255)                                                             atatTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATatgaatg                                                                                                                                                                                             <  1:334102/240‑1 (MQ=255)                                                               atTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATatgaatg                                                                                                                                                                                             <  1:95389/238‑1 (MQ=255)                                                                  gTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTAt                                                                                                                                                                                                        >  1:628871/1‑224 (MQ=255)                                                                    ttttATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGc                                                                                                                                                                           <  1:742202/251‑1 (MQ=255)                                                                    ttttATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGc                                                                                                                                                                           <  1:170118/251‑1 (MQ=255)                                                                         tCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGc                                                                                                                                                                      <  1:819381/251‑1 (MQ=255)                                                                           ggCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATatgaatg                                                                                                                                                                                             <  1:29867/226‑1 (MQ=255)                                                                                           ccGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAg                                                                                                                                                      <  1:559508/249‑1 (MQ=255)                                                                                                ataCAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTAt                                                                                                                                                                                                        >  1:848501/1‑194 (MQ=255)                                                                                                     gTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTg                                                                                                                                          >  1:228431/1‑251 (MQ=255)                                                                                                         ggTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGgtgt                                                                                                                                      >  1:109292/1‑251 (MQ=255)                                                                                                          gTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCg                                                                                                                                                                                                                 <  1:558329/175‑1 (MQ=255)                                                                                                                aCATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGtc                                                                                                                                                   >  1:113110/1‑231 (MQ=255)                                                                                                                     aTCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCa                                                                                                                           >  1:275896/1‑250 (MQ=255)                                                                                                                     aTCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCAc                                                                                                                          <  1:185087/251‑1 (MQ=255)                                                                                                                      tCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACg                                                                                                                         <  1:794422/251‑1 (MQ=255)                                                                                                                                  aaTTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTAc                                                                                                             <  1:460251/251‑1 (MQ=255)                                                                                                                                               gatgaAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTtaat                                                                                                >  1:652760/1‑251 (MQ=255)                                                                                                                                                     aaaTATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGc                                                                                                                                                                           >  1:726640/1‑170 (MQ=255)                                                                                                                                                       atatCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAatcatc                                                                                        <  1:609461/251‑1 (MQ=255)                                                                                                                                                         atCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAgg                                                                                                                                                     >  1:805884/1‑188 (MQ=255)                                                                                                                                                           ccATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCAc                                                                                                                                                                    >  1:74921/1‑171 (MQ=255)                                                                                                                                                           ccATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGt                                                                                                                                                    <  1:677217/187‑1 (MQ=255)                                                                                                                                                                              atCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGc                                                                                                                                                                      <  1:604270/150‑1 (MQ=255)                                                                                                                                                                               tCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAatat                                                                                   >  1:554731/1‑232 (MQ=255)                                                                                                                                                                                       ttCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACgg                                                        <  1:54951/251‑1 (MQ=255)                                                                                                                                                                                                 cGTTGTCGGTTTTGCGGACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATatgaatg                                                                                                                                                                                             >  1:640216/1‑108 (MQ=255)                                                                                                                                                                                                            ttGCGCACATAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGcc                                   <  1:883830/251‑1 (MQ=255)                                                                                                                                                                                                                   caTAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGc                                                                                                                                                                           <  1:318351/108‑1 (MQ=255)                                                                                                                                                                                                                   caTAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTaa                            >  1:664841/1‑251 (MQ=255)                                                                                                                                                                                                                   caTAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTaa                            >  1:234084/1‑251 (MQ=255)                                                                                                                                                                                                                   caTAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTaa                            >  1:645723/1‑251 (MQ=255)                                                                                                                                                                                                                     tAACTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTAAAg                          >  1:587244/1‑251 (MQ=255)                                                                                                                                                                                                                        cTCCTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTgg                                                                                                                                         >  1:36199/1‑137 (MQ=255)                                                                                                                                                                                                                          ccTGATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCa                                                      >  1:674898/1‑218 (MQ=255)                                                                                                                                                                                                                             gATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATaaa                                                                                             >  1:777638/1‑176 (MQ=255)                                                                                                                                                                                                                             gATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAat                                                                                     <  1:241268/184‑1 (MQ=255)                                                                                                                                                                                                                             gATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGcc                                   >  1:845336/1‑234 (MQ=255)                                                                                                                                                                                                                             gATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTAAAGGTGCAAtt                  >  1:206223/1‑251 (MQ=255)                                                                                                                                                                                                                             gATCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTAAAGGTGCAAtt                  >  1:517740/1‑251 (MQ=255)                                                                                                                                                                                                                              aTCAATACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAGTGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTgg                                                                                                                                         >  1:507818/1‑131 (MQ=255)                                                                                                                                                                                                                                  aTACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACgg                                                        >  1:506835/1‑208 (MQ=255)                                                                                                                                                                                                                                  aTACCTAAACGTTCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCt                              <  1:745920/234‑1 (MQ=255)                                                                                                                                                                                                                                             ttCGGCGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTAAAGGTGCAATTAACGTGGTATTTCcgc  <  1:860032/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TCTTGAAAACTAAGATTATGTTTAGCCAATTTTTCGGGTTCCAAACTGCGTAAAATCGCCATATTGTTTTTATCGGCGTCACTAAAAAAGCCGTTATACAGTTCGGTTTCAACATTATCGGAAGCGTCAAATTCACGTTCCTGATGAAAAATATCCATTACTTTTTGGCGAATATCTTTTGCTTCCTGCAAACGTTGTCGGTTTTGCGCACATAACTCCTGATCAATACCTAAACGTTCGGAGTTTTGTGGCAACAAAGTTTTAGCCGGCGCAACAATCGGACATTTATTGATATGAATGAGCTTCAATGGCACAGGCAATGCACCGGCAGCCAATAAGGTCTCTTTTTTGGTGTAAAGATTTTCACGCAACTCCCCTACCGATTTTGTTAATAAATCATCAATATCGGCAGTTAAATCACAGACAATAACGGCATTTTTATTCGTCGGATGCCAGGCTAAAGGTGCAATTAACGTGGTATTTCCGC  >  1/1272357‑1272843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑