Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,272,122	G→T	intergenic (+112542/+148815)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,272,122	0	G	T	100.0%	145.6 / NA	55	intergenic (+112542/+148815)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/26);  total (29/26)

TCACTATTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGTGCAGAGCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACGATAATGGAACAGCAATT  >  1/1271889‑1272342                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             tCACTATTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGattat                                                                                                                                                                                                             >  1:235117/1‑251 (MQ=25)      aTTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCg                                                                                                                                                                                                        >  1:22249/1‑251 (MQ=255)         ttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTc                                                                                                                                                                                                     <  1:843898/251‑1 (MQ=255)                    aGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATa                                                                                                                                                                                           <  1:200100/250‑1 (MQ=255)                      ggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAAc                                                                                                                                                                                                                    >  1:728771/1‑223 (MQ=18)                      ggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGa                                                                                                                                                                                         >  1:864185/1‑250 (MQ=255)                      ggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGa                                                                                                                                                                                         >  1:503538/1‑250 (MQ=255)                       gTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGAc                                                                                                                                                                                        <  1:752815/250‑1 (MQ=255)                       gTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGAc                                                                                                                                                                                        <  1:558549/250‑1 (MQ=255)                       gTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCCGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGAc                                                                                                                                                                                        <  1:429091/250‑1 (MQ=255)                       gTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCCGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGAc                                                                                                                                                                                        <  1:437133/250‑1 (MQ=255)                        tttATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCt                                                                                                                                                                                      <  1:524451/251‑1 (MQ=255)                           atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTc                                                                                                                                                                                                     <  1:634811/233‑1 (MQ=255)                           atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCtttt                                                                                                                                                                                   >  1:27890/1‑251 (MQ=255)                           atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAATTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCt                                                                                                                                                                                      <  1:587128/248‑1 (MQ=255)                                aGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGAc                                                                                                                                                                                        >  1:583539/1‑241 (MQ=255)                                    gATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCa                                                                                                                                                                                >  1:270491/1‑245 (MQ=255)                                          aCGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGAt                                                                                                                                                                    <  1:775909/251‑1 (MQ=255)                                             ccATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAAcc                                                                                                                                                                                                                   <  1:303474/201‑1 (MQ=18)                                             ccATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGAt                                                                                                                                                                    >  1:122950/1‑248 (MQ=255)                                               aTTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGAc                                                                                                                                                                                        >  1:150816/1‑226 (MQ=255)                                                              aCGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAatat                                                                                                                                                <  1:302902/251‑1 (MQ=255)                                                               cGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATA‑TCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTg                                                                                                                                              <  1:571705/251‑1 (MQ=255)                                                                 aaaGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGt                                                                                                                                             <  1:513464/251‑1 (MQ=255)                                                                   aGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGtt                                                                                                                                            >  1:878696/1‑250 (MQ=255)                                                                           ccAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGAt                                                                                                                                                                    >  1:192390/1‑218 (MQ=255)                                                                                  aTGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAg                                                                                                                            >  1:882102/1‑251 (MQ=255)                                                                                    gACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGa                                                                                                                           >  1:839570/1‑250 (MQ=255)                                                                                     aCTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTc                                                                                                                                  >  1:41881/1‑242 (MQ=255)                                                                                     aCTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGAc                                                                                                                          <  1:709458/250‑1 (MQ=255)                                                                                                              aTTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCa                                                                                                >  1:511985/1‑251 (MQ=255)                                                                                                                              gATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACgg                                                                                 >  1:759570/1‑250 (MQ=255)                                                                                                                                                   gACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTttcttc                                                                                                                                                             <  1:414361/153‑1 (MQ=255)                                                                                                                                                    aCCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAAtt                                                            <  1:293506/251‑1 (MQ=255)                                                                                                                                                        gTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAAttt                                                                                        <  1:115329/217‑1 (MQ=255)                                                                                                                                                        gTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTc                                                       >  1:383293/1‑250 (MQ=255)                                                                                                                                                                     ttAACGAAATTGATAGGAGTAAGACATAATCTGCCCCTAAAATTAGTTGTCCAGGTTTT‑‑GGGG‑GCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGt                                        <  1:366385/251‑1 (MQ=255)                                                                                                                                                                      tAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGt                                        <  1:648728/251‑1 (MQ=255)                                                                                                                                                                             aTTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAg                                 >  1:422271/1‑251 (MQ=255)                                                                                                                                                                             aTTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATGTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAg                                 <  1:808625/251‑1 (MQ=255)                                                                                                                                                                               tGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGaa                               >  1:172015/1‑251 (MQ=255)                                                                                                                                                                                 aTAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGaaa                              <  1:196967/250‑1 (MQ=255)                                                                                                                                                                                 aTAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGaaa                              <  1:733597/250‑1 (MQ=255)                                                                                                                                                                                     gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCt                                                                                                                                       <  1:899862/141‑1 (MQ=255)                                                                                                                                                                                     gagTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCACAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGt                           >  1:435839/1‑249 (MQ=255)                                                                                                                                                                                       gTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATg                            <  1:765983/246‑1 (MQ=255)                                                                                                                                                                                           gACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGAc                                                                                                                          >  1:607703/1‑148 (MQ=255)                                                                                                                                                                                           gACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACg                   >  1:359111/1‑251 (MQ=255)                                                                                                                                                                                            aCATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACGa                  >  1:563593/1‑251 (MQ=255)                                                                                                                                                                                               tAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGaaa                              <  1:408206/236‑1 (MQ=255)                                                                                                                                                                                                aaTCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAatat                                                                                                                                                >  1:51085/1‑121 (MQ=255)                                                                                                                                                                                                aaTCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACGATAAt              >  1:420961/1‑251 (MQ=255)                                                                                                                                                                                                           cAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCaa                                                                                                                 >  1:276561/1‑141 (MQ=255)                                                                                                                                                                                                           cAAATTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACGATAATGGAACAGCAAt   >  1:8479/1‑251 (MQ=255)                                                                                                                                                                                                             aaTTAGGTGTCCAGGTTTT‑‑GGGGGGCAGATCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACGATAATGGAACAGCAAtt  >  1:46209/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             TCACTATTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTT‑‑GGGGTGCAGAGCACTTTTAACCGATTATTTTCGTTCCATATTCATAGACCTTTTGCAATAACATAAGATTTTCTTCATTGTTGGAATATTGTTCGGCTAATTCTTGAAGACGTTGTTCAAATTGCAAAGCCTCGGCATCTAATTTTTTACGGCAGTATTTTTGCCATTTAATTTGTTCCGCACGGTTCAATGTACGATAGAAATGTCTGGCACGATAATGGAACAGCAATT  >  1/1271889‑1272342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑