Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,269,453	C→T	intergenic (+109873/+151484)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,269,453	0	C	T	100.0%	99.4 / NA	38	intergenic (+109873/+151484)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/15);  total (23/15)

AGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG‑CGCGCAACAAATAAAAATGCCCGAATAACATCGAGCATTATTTACCGGGCTGCAAGCCTTCGCCCTGCA‑TTTTCCGCAAGC‑GGAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGAC  >  1/1269271‑1269679                                                                                                                                                                                       |                                                                                                                                                                                                                                      ggTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCg                                                                                                                                                                   >  1:367120/2‑250 (MQ=255)                                gCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagcttgctcaaaaatagtt                                                                                                                                  >  1:236940/1‑230 (MQ=255)                                 ccTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagcttgcccaaaaatagtt                                                                                                                                  <  1:346280/250‑22 (MQ=255)                                   ttGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGc                                                                                                                                                                               >  1:828074/1‑204 (MQ=255)                                               taaataTTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACtt                                                                                                                    <  1:844796/251‑1 (MQ=255)                                                  atatTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCggg                                                                                                                                                                                >  1:530159/1‑188 (MQ=255)                                                        aTTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTaaaa                                                                                                           <  1:784907/251‑1 (MQ=255)                                                         tttATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                                                          >  1:268366/1‑251 (MQ=255)                                                           tATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGTAAATTTTTATTTCTTGATT‑AAATTTTGGGACGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTATCGGGCTGCAACCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCa                                                                                                        >  1:711642/1‑251 (MQ=255)                                                                 gACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                                                          >  1:426022/1‑243 (MQ=255)                                                                          aaaaatgtAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAg                                                                                         <  1:756241/246‑1 (MQ=255)                                                                           aaaatgtAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGt                                                                                        <  1:739068/247‑1 (MQ=255)                                                                                    gCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGGTTAAATTTAGGTA‑TTCGCAAAAAATAAAAATGCCCGAATAACAt                                                                                                                                                                                                  >  1:785375/1‑136 (MQ=255)                                                                                    gCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACAt                                                                                                                                                                                                  >  1:51778/1‑136 (MQ=255)                                                                                       tAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTc                                                                                             <  1:791023/234‑1 (MQ=255)                                                                                           ttCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGAtttt                                                                        >  1:341661/1‑251 (MQ=255)                                                                                                   cGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                                                       >  1:613501/1‑228 (MQ=255)                                                                                                    gTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                                                       <  1:303813/227‑1 (MQ=255)                                                                                                    gTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCAATTCTCGATTTAATTTATGGA‑CGCGGAAAAAATAAAAATGCCCGAATAACATTCTGCCTGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGGAAAATCACTATATCTg                                                                                               >  1:226758/1‑219 (MQ=255)                                                                                                      aTCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCAatttcgttaaaaaactatcaaac                                                             <  1:297496/251‑20 (MQ=255)                                                                                                         gCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                                                       >  1:531319/1‑222 (MQ=255)                                                                                                              aTCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTatat                                                                                                  >  1:171824/1‑206 (MQ=255)                                                                                                              aTCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAg                                                     >  1:790901/1‑251 (MQ=255)                                                                                                                       gcagTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATg                                            >  1:498703/1‑251 (MQ=255)                                                                                                                          gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                                                          <  1:799159/186‑1 (MQ=255)                                                                                                                          gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCa                                                                                    <  1:400230/208‑1 (MQ=255)                                                                                                                          gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGaaa                                         >  1:41444/1‑251 (MQ=255)                                                                                                                           ttattaCGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGaaa                                         <  1:521567/250‑1 (MQ=255)                                                                                                                              ttaCGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAAcc                                     <  1:875811/251‑1 (MQ=255)                                                                                                                                     gcTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACAt                                                                                                                                                                                                  <  1:791278/86‑1 (MQ=255)                                                                                                                                     gcTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTTAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCa                              >  1:484113/2‑251 (MQ=255)                                                                                                                                     gcTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACCAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCa                              >  1:575145/2‑251 (MQ=255)                                                                                                                                      cTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttg                                                                                                                                                     >  1:233728/1‑128 (MQ=255)                                                                                                                                        ttAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTc                                                                                             >  1:723725/1‑185 (MQ=255)                                                                                                                                               tATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagc                                                                                                                                                  >  1:720040/1‑119 (MQ=255)                                                                                                                                                  ttGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGttattt                 >  1:689263/1‑251 (MQ=255)                                                                                                                                                        gggCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTtattttattt            <  1:712035/250‑1 (MQ=255)                                                                                                                                                                 aTTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGac  <  1:850041/251‑1 (MQ=255)                                                                                                                                                                                       |                                                                                                                                                                                                                                      AGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG‑CGCGCAACAAATAAAAATGCCCGAATAACATCGAGCATTATTTACCGGGCTGCAAGCCTTCGCCCTGCA‑TTTTCCGCAAGC‑GGAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACCTGTTTCACCTTCCGTTATTTTATTTTCAGCCAGAC  >  1/1269271‑1269679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑