Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,269,403	C→G	intergenic (+109823/+151534)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,269,403	0	C	G	100.0%	93.9 / NA	36	intergenic (+109823/+151534)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (21/15);  total (21/15)

TTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG‑CGCGCAACAAATAAAAATGCCCGAATAACATCGAGCATTATTTACCGGGCTGCAAGCCTTCGCCCTGCA‑TTTTCCGCAAGC‑GGAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACC  >  1/1269162‑1269644                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     ttACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                                                                                                                                                                                                                                             <  1:814500/251‑1 (MQ=255)    ccATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTAt                                                                                                                                                                                                                                          >  1:334688/1‑251 (MQ=255)                    aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGaaa                                                                                                                                                                                                                          >  1:641107/1‑251 (MQ=255)                       taataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAGTCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAAttt                                                                                                                                                                                                                       >  1:358946/1‑251 (MQ=255)                        aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                                                                                                                                                                                                                                             >  1:332040/1‑228 (MQ=255)                                                                                       gTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                                                                                                                                                                                                                                             <  1:520655/165‑1 (MQ=255)                                                                                          aTTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTaaatttaggta                                                                                                                                                                                               <  1:749381/208‑9 (MQ=255)                                                                                                              ggTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCg                                                                                                                                >  1:367120/2‑250 (MQ=255)                                                                                                                                             gCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagcttgctcaaaaatagtt                                                                                               >  1:236940/1‑230 (MQ=255)                                                                                                                                              ccTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACA‑tttttgagcttgcccaaaaatagtt                                                                                               <  1:346280/250‑22 (MQ=255)                                                                                                                                                ttGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGc                                                                                                                                            >  1:828074/1‑204 (MQ=255)                                                                                                                                                            taaataTTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACtt                                                                                 <  1:844796/251‑1 (MQ=255)                                                                                                                                                               atatTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCggg                                                                                                                                             >  1:530159/1‑188 (MQ=255)                                                                                                                                                                     aTTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTaaaa                                                                        <  1:784907/251‑1 (MQ=255)                                                                                                                                                                      tttATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                       >  1:268366/1‑251 (MQ=255)                                                                                                                                                                        tATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGTAAATTTTTATTTCTTGATT‑AAATTTTGGGACGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTATCGGGCTGCAACCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCa                                                                     >  1:711642/1‑251 (MQ=255)                                                                                                                                                                              gACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                       >  1:426022/1‑243 (MQ=255)                                                                                                                                                                                       aaaaatgtAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAg                                                      <  1:756241/246‑1 (MQ=255)                                                                                                                                                                                        aaaatgtAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGt                                                     <  1:739068/247‑1 (MQ=255)                                                                                                                                                                                                 gCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGGTTAAATTTAGGTA‑TTCGCAAAAAATAAAAATGCCCGAATAACAt                                                                                                                                                               >  1:785375/1‑136 (MQ=255)                                                                                                                                                                                                 gCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACAt                                                                                                                                                               >  1:51778/1‑136 (MQ=255)                                                                                                                                                                                                    tAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTc                                                          <  1:791023/234‑1 (MQ=255)                                                                                                                                                                                                        ttCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGAtttt                                     >  1:341661/1‑251 (MQ=255)                                                                                                                                                                                                                cGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                    >  1:613501/1‑228 (MQ=255)                                                                                                                                                                                                                 gTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                    <  1:303813/227‑1 (MQ=255)                                                                                                                                                                                                                 gTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCAATTCTCGATTTAATTTATGGA‑CGCGGAAAAAATAAAAATGCCCGAATAACATTCTGCCTGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGGAAAATCACTATATCTg                                                            >  1:226758/1‑219 (MQ=255)                                                                                                                                                                                                                   aTCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCAatttcgttaaaaaactatcaaac                          <  1:297496/251‑20 (MQ=255)                                                                                                                                                                                                                      gCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTa                                                    >  1:531319/1‑222 (MQ=255)                                                                                                                                                                                                                           aTCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTatat                                                               >  1:171824/1‑206 (MQ=255)                                                                                                                                                                                                                           aTCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAg                  >  1:790901/1‑251 (MQ=255)                                                                                                                                                                                                                                    gcagTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATg         >  1:498703/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAAt                                                                       <  1:799159/186‑1 (MQ=255)                                                                                                                                                                                                                                       gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCa                                                 <  1:400230/208‑1 (MQ=255)                                                                                                                                                                                                                                       gTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGaaa      >  1:41444/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttattaCGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGaaa      <  1:521567/250‑1 (MQ=255)                                                                                                                                                                                                                                           ttaCGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAATTTAGGTA‑CGCGCAAAAAATAAAAATGCCCGAATAACATCGAGCATGGTTTACCGGGCTGCAAGCCTTCGCCCTACATTTTTGAGCTTGCTCAAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAAcc  <  1:875811/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     TTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG‑CGCGCAACAAATAAAAATGCCCGAATAACATCGAGCATTATTTACCGGGCTGCAAGCCTTCGCCCTGCA‑TTTTCCGCAAGC‑GGAAAATAGTTAGACAAGTTTCACTTGGAGTAAAATCACTATATCTGTCTTAGTATCATTTTTGGATTTTCCCGTTAAAAAACCTTTAGGAAGAAATGAAAAACC  >  1/1269162‑1269644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑