Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,269,271	A→G	intergenic (+109691/+151666)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,269,271	0	A	G	100.0%	74.8 / NA	27	intergenic (+109691/+151666)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

GAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAACCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG  >  1/1269026‑1269458                                                                                                                                                                                                                                                      |                                                                                                                                                                                            gaAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTaa                                                                                                                                                                                        >  1:807161/1‑251 (MQ=255)                   tctAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTgaag                                                                                                                                                                      <  1:313791/251‑1 (MQ=255)                    ctAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTgaaga                                                                                                                                                                     >  1:352855/1‑251 (MQ=255)                                                                                                tCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACg                                                                                          <  1:684168/250‑1 (MQ=255)                                                                                                   tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                                                                                                      <  1:661888/187‑1 (MQ=255)                                                                                                   tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTAt                                                                                       >  1:732398/1‑250 (MQ=255)                                                                                                                                         ttACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                                                <  1:814500/251‑1 (MQ=255)                                                                                                                                            ccATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTAt                                             >  1:334688/1‑251 (MQ=255)                                                                                                                                                            aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAATATCAATAAATATTGAtt                                                                                                                                    >  1:281235/1‑148 (MQ=255)                                                                                                                                                            aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAatat                                                                                                                                         <  1:796235/143‑1 (MQ=255)                                                                                                                                                            aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGaaa                             >  1:641107/1‑251 (MQ=255)                                                                                                                                                               taataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAGTCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAAttt                          >  1:358946/1‑251 (MQ=255)                                                                                                                                                               taataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATc                                                                         >  1:882711/1‑204 (MQ=255)                                                                                                                                                                aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                                                                                                      >  1:659191/1‑126 (MQ=255)                                                                                                                                                                aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATcagc                                                                      <  1:591573/206‑1 (MQ=255)                                                                                                                                                                aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                                                >  1:332040/1‑228 (MQ=255)                                                                                                                                                                 atatatGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                                                                                                      <  1:294688/125‑1 (MQ=255)                                                                                                                                                                 atatatGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCt                                                                             <  1:413914/198‑1 (MQ=255)                                                                                                                                                                 atatatGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATc                                                                         >  1:873395/1‑202 (MQ=255)                                                                                                                                                                     atgTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGc                                                                                                                                                             <  1:676356/112‑1 (MQ=255)                                                                                                                                                                                                      cgctacggattAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACAt                                                                                                                                                   <  1:502915/82‑1 (MQ=255)                                                                                                                                                                                                                 aataaaaaCACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAAc                                                                                                                                                     >  1:792150/5‑78 (MQ=255)                                                                                                                                                                                                                        acacAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCAt                                                                                  <  1:182044/138‑1 (MQ=255)                                                                                                                                                                                                                             gagTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATca                                                                        >  1:562196/1‑143 (MQ=255)                                                                                                                                                                                                                               gTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGAc                                                                                                                           >  1:324039/1‑90 (MQ=255)                                                                                                                                                                                                                               gTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                                                <  1:520655/165‑1 (MQ=255)                                                                                                                                                                                                                                  aTTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTaaatttaggta  <  1:749381/208‑9 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                            GAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAACCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTTTCATTTCTCGATTAAAATCATCAG  >  1/1269026‑1269458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑