Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,269,202	A→T	intergenic (+109622/+151735)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,269,202	0	A	T	100.0%	68.3 / NA	26	intergenic (+109622/+151735)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/12);  total (14/12)

GATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAACCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTT  >  1/1268959‑1269434                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         gATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTg                                                                                                                                                                                                                                    <  1:296838/250‑1 (MQ=255)  aTGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGAc                                                                                                                                                                                                                                  <  1:360393/251‑1 (MQ=255)                     cccTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGActtcgttaataaacgctacg                                                                                                                                                                                                               >  1:898298/1‑232 (MQ=255)                         ctATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCGCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGActtcgttaataaacgctacggatt                                                                                                                                                                                                           <  1:425117/251‑24 (MQ=255)                           aTGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGActtcgttaataaacgctacggattaa                                                                                                                                                                                                         >  1:385787/1‑226 (MQ=255)                                                  aaCCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTg                                                                                                                                                                                                                                    >  1:397864/1‑201 (MQ=255)                                                                    gaAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTaa                                                                                                                                                                >  1:807161/1‑251 (MQ=255)                                                                                      tctAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTgaag                                                                                                                                              <  1:313791/251‑1 (MQ=255)                                                                                       ctAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTgaaga                                                                                                                                             >  1:352855/1‑251 (MQ=255)                                                                                                                                                                   tCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACg                                                                  <  1:684168/250‑1 (MQ=255)                                                                                                                                                                      tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                                                                              <  1:661888/187‑1 (MQ=255)                                                                                                                                                                      tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTAt                                                               >  1:732398/1‑250 (MQ=255)                                                                                                                                                                                                            ttACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                        <  1:814500/251‑1 (MQ=255)                                                                                                                                                                                                               ccATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTAt                     >  1:334688/1‑251 (MQ=255)                                                                                                                                                                                                                               aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAATATCAATAAATATTGAtt                                                                                                            >  1:281235/1‑148 (MQ=255)                                                                                                                                                                                                                               aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAatat                                                                                                                 <  1:796235/143‑1 (MQ=255)                                                                                                                                                                                                                               aCATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGaaa     >  1:641107/1‑251 (MQ=255)                                                                                                                                                                                                                                  taataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAGTCAGCAGTTATTACGTGGCTTTAGGGGTATTTGCAGGGGCGGGAAAttt  >  1:358946/1‑251 (MQ=255)                                                                                                                                                                                                                                  taataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATc                                                 >  1:882711/1‑204 (MQ=255)                                                                                                                                                                                                                                   aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                                                                              >  1:659191/1‑126 (MQ=255)                                                                                                                                                                                                                                   aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATcagc                                              <  1:591573/206‑1 (MQ=255)                                                                                                                                                                                                                                   aataTATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGGCTTTAgggg                        >  1:332040/1‑228 (MQ=255)                                                                                                                                                                                                                                    atatatGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                                                                              <  1:294688/125‑1 (MQ=255)                                                                                                                                                                                                                                    atatatGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCt                                                     <  1:413914/198‑1 (MQ=255)                                                                                                                                                                                                                                    atatatGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATc                                                 >  1:873395/1‑202 (MQ=255)                                                                                                                                                                                                                                        atgTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGc                                                                                                                                     <  1:676356/112‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         GATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAACCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTATCGCATTATCTAATCAGCAGTTATTACGTGCCTTTAGGGGTATTTGCAGGGGCGGGAAATTT  >  1/1268959‑1269434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑