Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,269,141	C→T	intergenic (+109561/+151796)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,269,141	0	C	T	100.0%	79.0 / NA	31	intergenic (+109561/+151796)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

TAACTAAATTGTTAAGAGATTATTTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAACCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTAT  >  1/1268920‑1269373                                                                                                                                                                                                                              |                                                                                                                                                                                                                                         tAACTAAATTGTTAAGAGATTATTTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTc                                                                                                                                                                                                                                 >  1:9922/1‑231 (MQ=255)     tAAATTGTTAAGAGATTATTTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCatttattt                                                                                                                                                                                                         <  1:449538/251‑1 (MQ=255)        aTTGTTAAGAGATTATTTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCatttat                                                                                                                                                                                                           <  1:648706/246‑1 (MQ=255)           gTTAAGAGATTATTTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATtttattta                                                                                                                                                                                                   >  1:747323/1‑251 (MQ=255)                      attTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCatttattt                                                                                                                                                                                                         >  1:41805/1‑234 (MQ=255)                        ttAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatgt                                                                                                                                                                                      <  1:588803/251‑4 (MQ=255)                        ttAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatgt                                                                                                                                                                                      <  1:773672/251‑4 (MQ=255)                                      aGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatg                                                                                                                                                                                       <  1:5661/236‑3 (MQ=255)                                        gATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCa                                                                                                                                                                                                                >  1:882549/1‑209 (MQ=255)                                        gATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTg                                                                                                                                                                       <  1:296838/250‑1 (MQ=255)                                         aTGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGAc                                                                                                                                                                     <  1:360393/251‑1 (MQ=255)                                           gTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAatat                                                                                                                                                                                          <  1:360519/228‑1 (MQ=255)                                           gTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatg                                                                                                                                                                                       <  1:70602/231‑3 (MQ=255)                                                            cccTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGActtcgttaataaacgctacg                                                                                                                                                  >  1:898298/1‑232 (MQ=255)                                                                ctATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCGCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGActtcgttaataaacgctacggatt                                                                                                                                              <  1:425117/251‑24 (MQ=255)                                                                  aTGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAAtctc                                                                                                                                                                                                                                       >  1:707681/1‑160 (MQ=255)                                                                  aTGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGActtcgttaataaacgctacggattaa                                                                                                                                            >  1:385787/1‑226 (MQ=255)                                                                                    ttATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCa                                                                                                                                                                                                                >  1:878604/1‑165 (MQ=255)                                                                                         aaCCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTg                                                                                                                                                                       >  1:397864/1‑201 (MQ=255)                                                                                           ccTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCaaa                                                                                                                                                                                                                                    >  1:233195/1‑138 (MQ=255)                                                                                           ccTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAatat                                                                                                                                                                                          >  1:526854/1‑180 (MQ=255)                                                                                                           gaAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTaa                                                                                                   >  1:807161/1‑251 (MQ=255)                                                                                                              gTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCa                                                                                                                                                                                                                >  1:713089/1‑139 (MQ=255)                                                                                                               tATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatgt                                                                                                                                                                                      >  1:702760/1‑161 (MQ=255)                                                                                                                             tctAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTgaag                                                                                 <  1:313791/251‑1 (MQ=255)                                                                                                                              ctAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTgaaga                                                                                >  1:352855/1‑251 (MQ=255)                                                                                                                                                     catcatAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCatttattt                                                                                                                                                                                                         >  1:893031/1‑107 (MQ=255)                                                                                                                                                                                                          tCATTTTTAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACg     <  1:684168/250‑1 (MQ=255)                                                                                                                                                                                                             tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatgt                                                                                                                                                                                      >  1:599112/1‑67 (MQ=255)                                                                                                                                                                                                             tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAAtatatg                                                                                                                                                                                       <  1:141486/69‑3 (MQ=255)                                                                                                                                                                                                             tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTaa                                                                 <  1:661888/187‑1 (MQ=255)                                                                                                                                                                                                             tttttAAAGAGAGCAAATCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATATGTTATGCGAATTTAGTGACTTCGTTAATAAACGCTACGGATTAATAAAAACACAGAGTTATTCACAGGTCACTCTTACTGGTTAAACGTGATGGAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAAAAATGTAAGCATAAATTCAAGCACGTAt  >  1:732398/1‑250 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                         TAACTAAATTGTTAAGAGATTATTTAATGGAAACGGAAGGATGTACATAACGATAAAAACCCTCTATGAATATCAGTCGATTTTTATAAACCTAGATTCAGCTAGAGAAGTATTTTCACCTACCTCTAAAAACTCACGAATATCTCATCATCATAAACGCTCAAAAAATCAAATCTTCAAGAAATAAATTTTTTTACCCAGTCATTTTTAAAGAGAGCAAACCTCAAATTCCCAGAAGTGACTTACCATTTATTTTATTTAACATAATATAAGTTATGCGAAATTAGTGACAAAGTAAATGAACACTACGATTTAATGAAAACACAGAGTTATTCACAGGTCACTCTTACTAGTTAAACGTTATGAAAACTGAAGAAATAAAGCCTTGTAACATCAATAAATATTGATTTATCAAGACTTATTTAGAAGTGTAAGCATAAATTCAAGCACGTAT  >  1/1268920‑1269373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑