Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,267,807	G→A	intergenic (+108227/+153130)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,267,807	0	G	A	100.0%	185.6 / NA	69	intergenic (+108227/+153130)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/43);  total (26/43)

GCTTACCGCATTTTTTTATCGCACGTTTTATTATGAAGCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAGTTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTGCAGTAATGACAGCCAATG  >  1/1267560‑1268031                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 gCTTACCGCATTTTTTTATCGCACGTTTTATTATGAAGCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAAttt                                                                                                                                                                                                                               >  1:303464/1‑251 (MQ=255)          aTTTTTTTATCGCACGTTTTATTATGAAGCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAAt                                                                                                                                                                                                                      <  1:465249/251‑1 (MQ=255)          aTTTTTTTATCGCACGTTTTATTATGAAGCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAAt                                                                                                                                                                                                                      <  1:571268/251‑1 (MQ=255)                          ttttATTATGAAGCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAAtttt                                                                                                                                                                                                      >  1:577762/1‑251 (MQ=255)                                      gCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTg                                                                                                                                                                                           >  1:720431/1‑250 (MQ=255)                                          gTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGtt                                                                                                                                                                                      >  1:3771/1‑251 (MQ=255)                                           tGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGttc                                                                                                                                                                                     <  1:351329/251‑1 (MQ=255)                                            ggAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAACAATTTTTCTTCTTCGTGCTGtt                                                                                                                                                                                      >  1:740942/1‑249 (MQ=255)                                                  ggTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACg                                                                                                                                                                              >  1:462852/1‑251 (MQ=255)                                                   gTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                <  1:613390/248‑1 (MQ=255)                                                   gTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                <  1:373817/248‑1 (MQ=255)                                                   gTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                <  1:15738/248‑1 (MQ=255)                                                        ccaccGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCAt                                                                                                                                                                        <  1:56596/251‑1 (MQ=255)                                                          accGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTg                                                                                                                                                                      <  1:726615/251‑1 (MQ=255)                                                                  aaaCACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGt                                                                                                                                                              <  1:168805/251‑1 (MQ=255)                                                                         tGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGc                                                                                                                                                       <  1:50588/251‑1 (MQ=255)                                                                                   acCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTcc                                                                                                                                             <  1:789780/251‑1 (MQ=255)                                                                                   acCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTcc                                                                                                                                             <  1:676480/251‑1 (MQ=255)                                                                                    ccGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTcc                                                                                                                                             >  1:715874/1‑250 (MQ=255)                                                                                    ccGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTc                                                                                                                                              <  1:257531/249‑1 (MQ=255)                                                                                       cACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                <  1:141624/212‑1 (MQ=255)                                                                                        aCTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTtctc                                                                                                                                        <  1:801525/251‑1 (MQ=255)                                                                                              tGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCaaa                                                                                                                                  <  1:596680/251‑1 (MQ=255)                                                                                                  aaTAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATg                                                                                                                                <  1:12879/249‑1 (MQ=255)                                                                                                      aaaaTTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTac                                                                                                                          <  1:410668/251‑1 (MQ=255)                                                                                                       aaaTTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTtc                                                                                                                                          >  1:471609/1‑234 (MQ=255)                                                                                                             aGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTttattctcagaaagtgcggtgt                                                                                                                                                                                 <  1:265622/189‑16 (MQ=255)                                                                                                                     aaTGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGa                                                                                                           <  1:765217/251‑1 (MQ=255)                                                                                                                     aaTGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGa                                                                                                           <  1:272938/251‑1 (MQ=255)                                                                                                                       tGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGAtt                                                                                                         <  1:876061/251‑1 (MQ=255)                                                                                                                          gaagaagaaagGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTg                                                                                                      >  1:559018/5‑251 (MQ=255)                                                                                                                                    ggCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTtc                                                                                                                                          <  1:689739/205‑1 (MQ=255)                                                                                                                                    ggCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCa                                                                                            >  1:395489/1‑251 (MQ=255)                                                                                                                                      cTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAAt                                                                                          <  1:224036/251‑1 (MQ=255)                                                                                                                                        aaaTCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGagt                                                                                                                                                  >  1:178646/1‑193 (MQ=255)                                                                                                                                        aaaTCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAAttt                                                                                        >  1:460642/1‑251 (MQ=255)                                                                                                                                              aTGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATg                                                                                  <  1:767376/251‑1 (MQ=255)                                                                                                                                              aTGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATg                                                                                  <  1:324304/251‑1 (MQ=255)                                                                                                                                                  gACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                <  1:381400/153‑1 (MQ=255)                                                                                                                                                   aCTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGt                                                                             >  1:765711/1‑251 (MQ=255)                                                                                                                                                      tAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATg                                                                          <  1:800420/251‑1 (MQ=255)                                                                                                                                                        gCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCAt                                                                                                                                                                        >  1:795184/1‑155 (MQ=255)                                                                                                                                                              tatttaAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                >  1:42056/1‑141 (MQ=255)                                                                                                                                                               atttaAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGagtag                                                                                                                                                >  1:85106/1‑172 (MQ=255)                                                                                                                                                               atttaAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCaa                                                                 >  1:62916/1‑251 (MQ=255)                                                                                                                                                                   aaaCCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCAt                                                                                                                                                                        >  1:63176/1‑144 (MQ=255)                                                                                                                                                                   aaaCCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTAc                                                             <  1:83611/251‑1 (MQ=255)                                                                                                                                                                   aaaCCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGAGTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTAc                                                             <  1:426406/251‑1 (MQ=255)                                                                                                                                                                    aaCCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTaa                                                                                                                                                                   <  1:259353/148‑1 (MQ=255)                                                                                                                                                                    aaCCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTac                                                                                                                          >  1:494397/1‑189 (MQ=255)                                                                                                                                                                             aGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTc                                                                                                                                     <  1:564521/169‑1 (MQ=255)                                                                                                                                                                              gTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCaa                                                                                                                                                                                <  1:146687/125‑1 (MQ=255)                                                                                                                                                                                                gcCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGc                                                                                                                                                       >  1:125776/1‑132 (MQ=255)                                                                                                                                                                                                      aTCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAATAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATg                                                                                  <  1:684380/195‑1 (MQ=255)                                                                                                                                                                                                      aTCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAAttt                                                                                        <  1:265647/189‑1 (MQ=255)                                                                                                                                                                                                       tCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTtcatc                         <  1:102757/251‑1 (MQ=255)                                                                                                                                                                                                        cTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTcc                                                                                                                                             <  1:310430/134‑1 (MQ=255)                                                                                                                                                                                                        cTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCaaa                                                                                                                                  <  1:634792/145‑1 (MQ=255)                                                                                                                                                                                                        cTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTtcatca                        >  1:810329/1‑251 (MQ=255)                                                                                                                                                                                                                 caaGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAAttt                                                                                                                                                                                                       <  1:687540/67‑1 (MQ=255)                                                                                                                                                                                                                  aaGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTGCAGTaa              <  1:472796/251‑1 (MQ=255)                                                                                                                                                                                                                    ggAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGgtg                                     >  1:424766/1‑226 (MQ=255)                                                                                                                                                                                                                     gAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCCATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTTCAGTAATGa           >  1:452220/1‑251 (MQ=255)                                                                                                                                                                                                                     gAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGtt                                                                        <  1:630959/190‑1 (MQ=255)                                                                                                                                                                                                                     gAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTGCAGTAATGa           >  1:818486/1‑251 (MQ=255)                                                                                                                                                                                                                            ccTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTtctc                                                                                                                                        >  1:156313/1‑119 (MQ=255)                                                                                                                                                                                                                            ccTTTTCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTTCAGTAATGACAGGCaa    >  1:126791/1‑251 (MQ=255)                                                                                                                                                                                                                              ttttCCATCTTCGCCAAGAAGATTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTATCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTGCAGTAATGACAGCCAATg  <  1:431927/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gaTTCAATTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGc                                                                                                                                                       <  1:547659/82‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 GCTTACCGCATTTTTTTATCGCACGTTTTATTATGAAGCCAGTGGAACAGGTCAACCACCGGCAAAAACACTTGAAAAAAACACCGCACTTTCTGAGAATAAAAATTAAGTTTTGGAATGAGAAAAAGAAAGGCTAAATCAATGTGACTTAGCCTTTTATTTAAACCGGACTAGTTGCTTTCTGCTAAAGCGCCTAAATCTTTATCAACAAGGAATAAACCTTTTCCATCTTCGCCAAGAAGATTCAGTTTATCCAAAATTCCGCTGAATAATTTTTCTTCTTCGTGCTGTTCTTCAACGTACCATTGTAAGAAGTTAAAAGCAGAGTAGTCCTTCTCTTCAAATGTTCTACCAACCAATTCATTGATTTTGGCAGTAATCAATTTTTCATGTTCGTATGTTAATTCAAGTACTTGTTTTAACGAAGCGAATTGGTGTGGCGGTTCATCAATTGCAGTAATGACAGCCAATG  >  1/1267560‑1268031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑