Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,267,275 C→G intergenic (+107695/+153662) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,267,2750CG96.8% 80.7 / ‑3.7 31intergenic (+107695/+153662)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (13/17);  minor base T (1/0);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

AACTCGGATGTTGCATATTCATAATGGCATCGAACCCCACCAAATCGCCCGGTAAATGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTCATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACATAAGTGAGGATATATGGCTGCTGAAAAAC  >  1/1267047‑1267513
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              
aaCTCGGATGTTGCATATTCATAATGGCATCGAACCCCACCAAATCGCCCGGTAAATGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACAt                                                                                                                                                                                                                          <  1:4953/251‑1 (MQ=255)
         gTTGCATATTCATAATGGCATCGAACCCCACCAAATCGCCCGGTAAATGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTa                                                                                                                                                                                                                 >  1:113197/1‑251 (MQ=255)
                        tGGCATCGAACCCCACCAAATCGCCCGGTAAATGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTgg                                                                                                                                                                                                  >  1:32738/1‑251 (MQ=255)
                                                   gTAAATGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCaattattacgcaaatgcccggtaccg                                                                                                                                                                       >  1:217632/1‑228 (MQ=255)
                                                     aaaTGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAAc                                                                                                                                                                     <  1:52833/251‑1 (MQ=255)
                                                                                                                              cGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAAt                                                                                                                                    >  1:20651/1‑211 (MQ=255)
                                                                                                                                                            tAAAAAGTACTTGGGATTACTATATAGGTTTTTGACGTTCAATAATATTATCTAACTGATCAAATTCGTATTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATACTAAAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACGCGACGTCCTAATTTTATTTCTGTCGCTAAAATTTTCATTTATGCCACAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGAACTAGCTCAATAGTAc                                                              <  1:293302/251‑1 (MQ=255)
                                                                                                                                                               aaaGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCAc                                                           <  1:374334/251‑1 (MQ=255)
                                                                                                                                                                     cTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTc                                                     <  1:251297/251‑1 (MQ=255)
                                                                                                                                                                               cTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGc                                                                                                                                                                                                             <  1:791157/89‑1 (MQ=255)
                                                                                                                                                                               cTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATc                                                                            <  1:901159/218‑1 (MQ=255)
                                                                                                                                                                                 gTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTaaga                                         >  1:146046/1‑251 (MQ=255)
                                                                                                                                                                                      ggTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGAt                                                                                                                                                                          >  1:854007/1‑117 (MQ=255)
                                                                                                                                                                                           tttACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACa                               <  1:18233/251‑1 (MQ=255)
                                                                                                                                                                                              aCGTTCAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTAc                                                              <  1:182106/217‑1 (MQ=255)
                                                                                                                                                                                                   cAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCAc                                                           <  1:719143/215‑1 (MQ=255)
                                                                                                                                                                                                   cAATAATATTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATATATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACt                                                          <  1:199040/216‑1 (MQ=255)
                                                                                                                                                                                                     ataataTTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAg                                                                 >  1:277919/1‑207 (MQ=255)
                                                                                                                                                                                                        atatTATCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAg                                                                                                            >  1:772809/1‑161 (MQ=255)
                                                                                                                                                                                                          attatCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACATAAGTg                         >  1:511618/1‑242 (MQ=255)
                                                                                                                                                                                                           ttatCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGa                                                                                                                                                                           <  1:599661/95‑1 (MQ=255)
                                                                                                                                                                                                           ttatCTAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAg                                                                                                            >  1:666493/1‑158 (MQ=255)
                                                                                                                                                                                                                tAACTGATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTa                                                         >  1:496473/1‑204 (MQ=255)
                                                                                                                                                                                                                     gATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTAc                                                              <  1:688226/194‑1 (MQ=255)
                                                                                                                                                                                                                     gATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTa                                                         >  1:616773/1‑199 (MQ=255)
                                                                                                                                                                                                                     gATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTaaga                                         <  1:356633/215‑1 (MQ=255)
                                                                                                                                                                                                                     gATCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACAt                              <  1:165178/226‑1 (MQ=255)
                                                                                                                                                                                                                      aTCTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACATAAGTGAGGATATATGgc             <  1:130730/242‑1 (MQ=255)
                                                                                                                                                                                                                       tCTAATTCGTGTTTATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAg                                                                 >  1:879155/1‑189 (MQ=255)
                                                                                                                                                                                                                        cTAATTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACATAAGTGAGGATATATGGCTGCTGAAAAAc  <  1:253194/251‑1 (MQ=255)
                                                                                                                                                                                                                          aaTTCGTGTTGATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACATAAGTGAGGATATATGGCTGCTGaaaaa   >  1:860914/1‑248 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              
AACTCGGATGTTGCATATTCATAATGGCATCGAACCCCACCAAATCGCCCGGTAAATGAAAAGAGGTAATTTGTTCTTCGCCTGTTTCGCTAATGGTATAAGATTTGATTGTGCCGGAACGGATAGCGTAGAGTGAGGTCAGCTCATCGCCCGCTTTAAAAAGTACTTGGGATTTCTGTATAGGTTTTTTACGTTCAATAATATTATCTAACTGATCTAATTCGTGTTCATTCAAAGTAAAAGGAATACATAGCTGGCTAATGCTACAATTTTGGCAATGAATGGCACACCCCCCGGATTGAACTCGACGTCCTAATTTTGTTTCTGTCGCTAAAATTTTCATTTATGCCCCAAATTACAGAAAAATTGAAAAAAAATCGTGTAAAATTGATCTAGCTCAATAGTACCACTAATTCATTAATTTAAGAAGTATAAACATAAGTGAGGATATATGGCTGCTGAAAAAC  >  1/1267047‑1267513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑