Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,262,669	G→A	intergenic (+103089/+158268)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,262,669	0	G	A	100.0%	179.8 / NA	66	intergenic (+103089/+158268)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/23);  total (43/23)

GTGGTAGGCTTTATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAGCCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATGCGTACCGACTCCACCAA  >  1/1262448‑1262905                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             gTGGTAGGCTTTATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTg                                                                                                                                                                                                                  >  1:766059/1‑250 (MQ=255)  tGGTAGGCTTTATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCg                                                                                                                                                                                                                >  1:60737/1‑251 (MQ=255)        gCTTTATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGATGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGAt                                                                                                                                                                                                          >  1:184291/1‑251 (MQ=255)        gCTTTATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGAt                                                                                                                                                                                                          >  1:411294/1‑251 (MQ=255)          tttATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGAttt                                                                                                                                                                                                        >  1:219532/1‑251 (MQ=255)             aTGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGAtttttt                                                                                                                                                                                                     >  1:112528/1‑251 (MQ=255)                gTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAg                                                                                                                                                                                                  >  1:897062/1‑251 (MQ=255)                    cACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTa                                                                                                                                                                                                    <  1:611728/245‑1 (MQ=255)                           aCTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGa                                                                                                                                                                                         >  1:672932/1‑249 (MQ=255)                                ggAAAAAAGCGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTgg                                                                                                                                                                                                            <  1:335965/225‑1 (MQ=255)                                  aaaaaaGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGAt                                                                                                                                                                                >  1:327439/1‑251 (MQ=255)                                                  gTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAAc                                                                                                                                                                 >  1:214147/1‑250 (MQ=255)                                                  gTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAAc                                                                                                                                                                 >  1:837138/1‑250 (MQ=255)                                                  gTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAAc                                                                                                                                                                 >  1:343237/1‑250 (MQ=255)                                                  gTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAAc                                                                                                                                                                 >  1:197025/1‑250 (MQ=255)                                                          gCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAGAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGa                                                                                                                                                         >  1:435811/1‑250 (MQ=255)                                                          gCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTAGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGa                                                                                                                                                         >  1:353418/1‑250 (MQ=255)                                                             ggTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAg                                                                                                                                                     >  1:721573/1‑251 (MQ=255)                                                              gTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGc                                                                                                                                                            >  1:721608/1‑243 (MQ=255)                                                              gTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGt                                                                                                                                                    >  1:725066/1‑251 (MQ=255)                                                                  tgtgCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTcc                                                                                                                                                 <  1:196798/250‑1 (MQ=255)                                                                         tCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAag                                                                                                                                         <  1:385479/251‑1 (MQ=255)                                                                                      aGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAgcgc                                                                                                                                    <  1:277025/243‑1 (MQ=255)                                                                                      aGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTttatt                                                                                                                            >  1:182293/1‑251 (MQ=255)                                                                                        ttAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTttatt                                                                                                                            <  1:867127/249‑1 (MQ=255)                                                                                         tAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTAc                                                                                                                          >  1:230624/1‑250 (MQ=255)                                                                                           aTCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTc                                                                                                                       <  1:569755/251‑1 (MQ=255)                                                                                                              aaaTTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAagag                                                                                                                                       <  1:768199/216‑1 (MQ=255)                                                                                                              aaaTTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGAGGTTCAATTTAAAGGTAAGAAATTCGAACCGAACAATGc                                                                                                                                                                                                                                   >  1:810729/1‑124 (MQ=255)                                                                                                                                    ggAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCGAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGgtg                                                                              >  1:443897/1‑251 (MQ=255)                                                                                                                                     gAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTacaac                                                                                                            >  1:600325/1‑220 (MQ=255)                                                                                                                                         atTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTAtg                                                                                                                                                                                    <  1:646420/144‑1 (MQ=255)                                                                                                                                                 gTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCat                                                                 >  1:467360/1‑251 (MQ=255)                                                                                                                                                       gTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGtt                                                           >  1:379448/1‑251 (MQ=255)                                                                                                                                                         gCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGc                                                                                     >  1:199912/1‑223 (MQ=255)                                                                                                                                                           tCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGgcg                                                       <  1:199623/251‑1 (MQ=255)                                                                                                                                                            cACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGc                                                                                     <  1:615935/220‑1 (MQ=255)                                                                                                                                                            cACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGgcg                                                       >  1:203580/1‑250 (MQ=255)                                                                                                                                                            cACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGgcg                                                       >  1:891678/1‑250 (MQ=255)                                                                                                                                                            cACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAA‑CAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCa                                                     >  1:697664/1‑251 (MQ=255)                                                                                                                                                              ccAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAGAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCaa                                                    >  1:274609/1‑251 (MQ=255)                                                                                                                                                              ccAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTAtgt                                                                                                                                                                                   <  1:406832/124‑1 (MQ=255)                                                                                                                                                              ccAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGgtg                                                                              <  1:34581/225‑1 (MQ=255)                                                                                                                                                              ccAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCaa                                                    >  1:465439/1‑251 (MQ=255)                                                                                                                                                                 aTACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTacaac                                                                                                            >  1:555332/1‑192 (MQ=255)                                                                                                                                                                     acCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGt                                             >  1:467270/1‑251 (MQ=255)                                                                                                                                                                      ccGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTa                                            <  1:72561/251‑1 (MQ=255)                                                                                                                                                                         aaaGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATg                                          <  1:46953/250‑1 (MQ=255)                                                                                                                                                                                   aaaTTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTAc                               <  1:701561/251‑1 (MQ=255)                                                                                                                                                                                     aTTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACAt                             <  1:872560/251‑1 (MQ=255)                                                                                                                                                                                     aTTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACAt                             <  1:218198/251‑1 (MQ=255)                                                                                                                                                                                      ttCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATc                            <  1:121586/251‑1 (MQ=255)                                                                                                                                                                                        cGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCa                           >  1:639982/1‑250 (MQ=255)                                                                                                                                                                                          cTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACt                         <  1:589139/250‑1 (MQ=255)                                                                                                                                                                                            tGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTtat                      >  1:191140/1‑251 (MQ=255)                                                                                                                                                                                              ggtgtgGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCCAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCCCTTatat                    >  1:871106/3‑251 (MQ=255)                                                                                                                                                                                              gATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTacaac                                                                                                            >  1:349738/1‑163 (MQ=255)                                                                                                                                                                                              gATGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTatat                    >  1:871133/1‑251 (MQ=255)                                                                                                                                                                                               aTGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAGCCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATg                   <  1:782778/251‑1 (MQ=255)                                                                                                                                                                                               aTGTGGTTCAATTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATg                   >  1:321265/1‑251 (MQ=255)                                                                                                                                                                                                         aTTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTttatt                                                                                                                            <  1:31179/136‑1 (MQ=255)                                                                                                                                                                                                         aTTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTa                                            >  1:847776/1‑216 (MQ=255)                                                                                                                                                                                                         aTTTAAAGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATGCGTACCGa           >  1:665236/1‑249 (MQ=255)                                                                                                                                                                                                              aaGGTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATGCGTACCGACTccacc    <  1:147243/251‑1 (MQ=255)                                                                                                                                                                                                                ggTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTAt                                           >  1:66148/1‑210 (MQ=255)                                                                                                                                                                                                                ggTAAGAAATTCGAACCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATGCGTACCGACTCCACCaa  >  1:261776/1‑251 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             GTGGTAGGCTTTATGGTCTCACCGTTACTTTGGAAAAAAGTGGCGCGCGGTTTATCTGCCGGTCGTGTGCAATCGGTGGCGGTGAAGTTAATCGTTGAGCGTGAACGTGAAATTAAGGCTTTCGTGCCACAGGAATATTGGGAAGTCTCCGTGCTCACCAATACACCGAAAGGCGATGAAATTCGCTTGGATGTGGTTCAATTTAAAGGTAAGAAATTCGAGCCGAAAAATGCCGAGCAGGCGCAAAGTGCGGTGGATTTTTTAAGTAAATCCGACTATGTGATTTCCGATTTGGAAACCAAGCCGACCAGTTCCCGCCCAAGAGCGCCGTTTATTACCTCAACCTTACAACAAACCGCTAGCACCCGTTTAGGCTTCGGTGTGAAAAAAACCATGATGTTGGCGCAACGTTTGTATGAAGCCGGTTACATCACTTATATGCGTACCGACTCCACCAA  >  1/1262448‑1262905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑