Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,259,609	A→G	intergenic (+100029/+161328)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,259,609	0	A	G	100.0%	173.3 / NA	64	intergenic (+100029/+161328)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/28);  total (36/28)

TTTGCTTTGCAAGCCAGCCGCCCGGAAGAAAATGTGTTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGTCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAATTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAGGGATTTTCGCCCTTGATGC  >  1/1259366‑1259844                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            tttgctttgcAAGCCAGCCGCCCGGAAGAAAATGTGTTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGttt                                                                                                                                                                                                                                      <  1:793036/251‑1 (MQ=255)     tggtgtaaGCCAGCCGCCCGGAAGAAAATGTGTTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTc                                                                                                                                                                                                                                  <  1:173861/245‑1 (MQ=255)                         gaagaaAATGTGTTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCa                                                                                                                                                                                                              >  1:708715/1‑251 (MQ=255)                             aaaaTGTGTTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAAc                                                                                                                                                                                                           <  1:276815/250‑1 (MQ=255)                                  gtgtTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTtgat                                                                                                                                                                                                     >  1:887760/1‑251 (MQ=255)                                  gtgtTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTtgat                                                                                                                                                                                                     >  1:518287/1‑251 (MQ=255)                                  gtgtTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACAGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTtgat                                                                                                                                                                                                     >  1:312220/1‑251 (MQ=255)                                       gAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGaaa                                                                                                                                                                                                 >  1:805990/1‑250 (MQ=255)                                       gAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGaaa                                                                                                                                                                                                 >  1:78362/1‑250 (MQ=255)                                            aTGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATgcgc                                                                                                                                                                                                                     <  1:770895/225‑1 (MQ=255)                                                    cAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAAc                                                                                                                                                                                    <  1:558822/250‑1 (MQ=255)                                                    cAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAAc                                                                                                                                                                                    <  1:760615/250‑1 (MQ=255)                                                    cAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAAc                                                                                                                                                                                    <  1:278047/250‑1 (MQ=255)                                                    cAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACg                                                                                                                                                                                   >  1:546197/1‑251 (MQ=255)                                                     aGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGt                                                                                                                                                                                  >  1:836732/1‑251 (MQ=255)                                                      gTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACg                                                                                                                                                                                   <  1:210809/249‑1 (MQ=255)                                                      gTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTa                                                                                                                                                                                 >  1:654556/1‑251 (MQ=255)                                                        tAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATc                                                                                                                                                                               <  1:617875/251‑1 (MQ=255)                                                                      aGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGa                                                                                                                                                                 >  1:85904/1‑251 (MQ=255)                                                                          tcaatttcaatAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTa                                                                                                                                                             >  1:674353/1‑251 (MQ=255)                                                                                       tCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACAGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGt                                                                                                                                                >  1:535957/1‑251 (MQ=255)                                                                                           aaTCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACg                                                                                                                                             <  1:384789/250‑1 (MQ=255)                                                                                               gtgtACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAAc                                                                                                                                                                                                           >  1:334231/1‑184 (MQ=255)                                                                                               gtgtACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGaaa                                                                                                                                        >  1:667919/1‑251 (MQ=255)                                                                                                                 gCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAAc                                                                                                                                                                                    <  1:893150/189‑1 (MQ=255)                                                                                                                       tCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAatat                                                                                                                <  1:476600/251‑1 (MQ=255)                                                                                                                       tCGCAGGGCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAatat                                                                                                                <  1:828439/251‑1 (MQ=255)                                                                                                                            gggCAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCaaaa                                                                                                           >  1:38128/1‑251 (MQ=255)                                                                                                                               cAAAATTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAg                                                                                                         <  1:897379/250‑1 (MQ=255)                                                                                                                                 aaaTTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAg                                                                                                                      <  1:792241/235‑1 (MQ=255)                                                                                                                                   aTTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATc                                                                                                                                     <  1:211880/218‑1 (MQ=255)                                                                                                                                   aTTTAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTaa                                                                                                                   >  1:470864/1‑236 (MQ=255)                                                                                                                                    tttAAAAGCCTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGt                                                                                                   >  1:506819/1‑251 (MQ=255)                                                                                                                                             cTTCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGa                                                                                          >  1:659173/1‑251 (MQ=255)                                                                                                                                              ttCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAAc                                                                                                                                                                                    >  1:859373/1‑160 (MQ=255)                                                                                                                                              ttCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAg                                                                                                                      >  1:414760/1‑222 (MQ=255)                                                                                                                                              ttCCATGCCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAatat                                                                                                                >  1:568550/1‑228 (MQ=255)                                                                                                                                                    gccATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGaaaa                                                                                                                                >  1:16932/3‑206 (MQ=255)                                                                                                                                                     ccATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGt                                                                                                                                                                                                         >  1:84929/2‑132 (MQ=255)                                                                                                                                                              gTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTc                                                                          >  1:889719/1‑250 (MQ=255)                                                                                                                                                                       aTCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAAc                                                                <  1:673743/251‑1 (MQ=255)                                                                                                                                                                        tCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGt                                                                                                                     >  1:252197/1‑197 (MQ=255)                                                                                                                                                                         cGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTa                                                              <  1:214356/251‑1 (MQ=255)                                                                                                                                                                                 aaCGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGGGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGgaga                                                      <  1:254103/251‑1 (MQ=255)                                                                                                                                                                                   cGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGgagaga                                                    >  1:508573/1‑251 (MQ=255)                                                                                                                                                                                        atTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAGAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATaaa                                                >  1:441118/1‑250 (MQ=255)                                                                                                                                                                                         ttttGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACg                                              <  1:263022/251‑1 (MQ=255)                                                                                                                                                                                               ttAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAgtatg                                        <  1:360416/251‑1 (MQ=255)                                                                                                                                                                                                 aaaCTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGaa                                                                                                                                         <  1:535880/152‑1 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAAc                                                                                                                                                                                    >  1:869695/1‑104 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGgaga                                                      <  1:59335/230‑1 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGgag                                                       >  1:736347/1‑229 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACg                                              <  1:355390/238‑1 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAg                                            <  1:350947/240‑1 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTAtt                                    >  1:192188/1‑248 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCtt                                 >  1:634943/1‑251 (MQ=255)                                                                                                                                                                                                      gAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCtt                                 >  1:426858/1‑251 (MQ=255)                                                                                                                                                                                                            aaGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGAtt                            >  1:634763/1‑250 (MQ=255)                                                                                                                                                                                                                    cGCAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGATTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAggg                   >  1:450885/1‑251 (MQ=255)                                                                                                                                                                                                                      cAAGTAGCTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAGGGa                  <  1:363289/250‑1 (MQ=255)                                                                                                                                                                                                                             cTTCTCGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAGGGATTTTCGcc          <  1:621580/251‑1 (MQ=255)                                                                                                                                                                                                                                 tcGCTTAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTaa                                                                                                                   <  1:770991/142‑1 (MQ=255)                                                                                                                                                                                                                                      tAATTGAACCGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAGGGATTTTCGCCCTTGATGc  >  1:850736/1‑250 (MQ=255)                                                                                                                                                                                                                                              ccGTTAGTTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAg                     >  1:341154/1‑223 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TTTGCTTTGCAAGCCAGCCGCCCGGAAGAAAATGTGTTGAACAATGTGATGCAGTTAATGGAGCACCCGAGCTTCAATTTCAATAATCCGAATCGTGTACGTTCGTTAGTCGGCACATTCGCAGGGCAAAATTTAAAAGCCTTCCATGTCATTGACGGTTCCGGCTATCGTTTCTTAACGGATATTTTGATTAAACTGAATAAAAGCAACCCGCAAGTAGCTTCTCGCTTAATTGAACCGTTAATTCGTTTTGTCCGTTATGATGCGCAACGCCAAACGTTGATGAAACGCGCCTTAGAACGTATCAGCGAAACGGAAGATTTATCCCGCGATTTGTACGAGAAAATCGAAAAAGCATTACAGTAATATCAAAAAGTGCGGTTAATTTTGACCGCACTTTTTCATTCCACAGTTAACTATCCGGAGAGATAAACGAGTATGTATTCTTTGATTCGTAAAGGGATTTTCGCCCTTGATGC  >  1/1259366‑1259844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑