Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,258,324	T→C	intergenic (+98744/+162613)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,258,324	0	T	C	100.0%	186.3 / NA	67	intergenic (+98744/+162613)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/27);  total (40/27)

TAAAAGAAGGCTTGACGGTTTTTCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTTCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCACCGACCGCCGACCAAATG  >  1/1258077‑1258552                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     tAAAAGAAGGCTTGACGGTTTTTCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCaa                                                                                                                                                                                                                                   <  1:674172/251‑1 (MQ=255)         ggCTTGACGGTTTTTCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCAt                                                                                                                                                                                                                           >  1:841475/1‑251 (MQ=255)          gCTTGACGGTTTTTCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATg                                                                                                                                                                                                                          >  1:34869/1‑251 (MQ=255)             tGACGGTTTTTCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAg                                                                                                                                                                                                                       <  1:820314/251‑1 (MQ=255)                   tttttCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATAc                                                                                                                                                                                                                 <  1:59989/251‑1 (MQ=255)                   tttttCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATAc                                                                                                                                                                                                                 <  1:288666/251‑1 (MQ=255)                   tttttCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGACGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATAc                                                                                                                                                                                                                 <  1:892787/251‑1 (MQ=255)                            aTCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGaa                                                                                                                                                                                                        >  1:553169/1‑251 (MQ=255)                              cAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATAc                                                                                                                                                                                                                 <  1:175730/240‑1 (MQ=255)                                                                            ataaCGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGaa                                                                                                                                                        <  1:350694/251‑1 (MQ=255)                                                                                      aatCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATg                                                                                                                                              <  1:374024/249‑1 (MQ=255)                                                                                      aTTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATg                                                                                                                                              <  1:717199/251‑1 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATAc                                                                                                                                                                                                                 <  1:330642/181‑1 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGaa                                                                                                                                                        >  1:192712/1‑238 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATg                                                                                                                                              >  1:358371/1‑248 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGt                                                                                                                                           >  1:250824/1‑251 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGt                                                                                                                                           >  1:552479/1‑251 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGt                                                                                                                                           >  1:230825/1‑251 (MQ=255)                                                                                         cTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGt                                                                                                                                           >  1:822203/1‑251 (MQ=255)                                                                                                      cAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAAccc                                                                                                                               <  1:487586/250‑1 (MQ=255)                                                                                                                         gCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAGGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAg                                                                                                            >  1:447935/1‑250 (MQ=255)                                                                                                                          cccGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGt                                                                                                                                                     <  1:863260/208‑1 (MQ=255)                                                                                                                          cccGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGc                                                                                                           >  1:283628/1‑250 (MQ=255)                                                                                                                          cccGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGc                                                                                                           >  1:781984/1‑250 (MQ=255)                                                                                                                                  cacaTCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCgg                                                                                                   <  1:375265/250‑1 (MQ=255)                                                                                                                                    caTCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCgg                                                                                                   >  1:333069/1‑248 (MQ=255)                                                                                                                                    caTCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTa                                                                                                 >  1:649237/1‑250 (MQ=255)                                                                                                                                      tCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAgcgg                                                                                                                                                                                        <  1:28916/161‑1 (MQ=255)                                                                                                                                            tCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAAt                                                                                        <  1:177398/251‑1 (MQ=255)                                                                                                                                            tCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAAt                                                                                        <  1:87067/251‑1 (MQ=255)                                                                                                                                                                    gAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGaa                                                                                                                                                        >  1:722076/1‑163 (MQ=255)                                                                                                                                                                    gAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACg                                                                >  1:891941/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGGGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGa                                                                           <  1:734648/234‑1 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAAccc                                                                                                                               <  1:679094/182‑1 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCgg                                                                                                   >  1:451545/1‑210 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:459885/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:501980/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:80591/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:791359/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:57860/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:164865/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaaa                                                          >  1:83561/1‑251 (MQ=255)                                                                                                                                                                          tttCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCG‑AAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGcc                                                                                                          <  1:212983/202‑1 (MQ=255)                                                                                                                                                                            tCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCaa                                                                                                                                                                                                                                   <  1:646937/80‑1 (MQ=255)                                                                                                                                                                            tCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAAt                                                        <  1:834183/251‑1 (MQ=255)                                                                                                                                                                            tCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTAGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATg                                                                   >  1:428083/1‑240 (MQ=255)                                                                                                                                                                                  cGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCAtgtg                                                  >  1:561987/1‑251 (MQ=255)                                                                                                                                                                                    gTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTa                                                >  1:119959/1‑251 (MQ=255)                                                                                                                                                                                      gACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTAt                                               >  1:420573/1‑250 (MQ=255)                                                                                                                                                                                        ccGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCaa                                            >  1:263484/1‑251 (MQ=255)                                                                                                                                                                                                       gcgcAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCg                                                                                                                     >  1:422965/1‑163 (MQ=255)                                                                                                                                                                                                       gcgcAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTtctc                               <  1:617561/249‑1 (MQ=255)                                                                                                                                                                                                        cgcAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTTCAGCCAATCCGGTACGCCTGAATTGACCATCAGCGGTCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAAt                            <  1:881400/251‑1 (MQ=255)                                                                                                                                                                                                         gcAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGcc                                                                                                          >  1:230727/1‑172 (MQ=255)                                                                                                                                                                                                            gAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTaa                         >  1:336144/1‑250 (MQ=255)                                                                                                                                                                                                                   ttCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGAt                                                                                                                                      <  1:5504/134‑1 (MQ=255)                                                                                                                                                                                                                   ttCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAAt                                                                                        <  1:692143/180‑1 (MQ=255)                                                                                                                                                                                                                   ttCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAAt                                           <  1:712120/225‑1 (MQ=255)                                                                                                                                                                                                                   ttCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCaccg                 >  1:96373/1‑251 (MQ=255)                                                                                                                                                                                                                   ttCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCaccg                 >  1:603180/1‑251 (MQ=255)                                                                                                                                                                                                                   ttCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCaccg                 >  1:247846/1‑251 (MQ=255)                                                                                                                                                                                                                          gTTGCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCgg                                                                                                   >  1:130104/1‑162 (MQ=255)                                                                                                                                                                                                                             gCACACCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAAt                                                                                        <  1:258461/170‑1 (MQ=255)                                                                                                                                                                                                                                cacCTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATg                                                                   >  1:173767/1‑188 (MQ=255)                                                                                                                                                                                                                                  ccTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATTGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGCACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGaaa                                                           >  1:274127/1‑194 (MQ=255)                                                                                                                                                                                                                                  ccTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCACCGACCGCCGACCAAATg  >  1:552629/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccTTATTGGGCGAAAAAGGCTTCCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCACCGACCGCCGACCAAATg  >  1:417884/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     TAAAAGAAGGCTTGACGGTTTTTCGCGATCAGGAATTTTCCTCCGACACCGGCTCACGCCCGGTAAATCGCATTAATAACGTGAAATTCTTACGCACCGTGCAATTCGCCGAGGATGCCGGCCCGATGGCACATCCGATTCGTCCGGAAAAAGTGATTGAAATGAATAATTTCTATACGGTGACCGTGTATGAAAAAGGCGCAGAAGTCATTCGTATGTTGCACACCTTATTGGGCGAAAAAGGCTTTCAAAAAGGCATGAAGTTATACATCGCGGAAAATGACGGCAAAGCGGCGACCTGTGAAGATTTTGTTTCTGCGATGGAACGTGCCAATGAGTTGGATTTAACCCAATTTCGCCGTTGGTACAGCCAATCCGGTACGCCTGAATTGACCATCAGCGATCGTTATGACGAGAAAAATCATGTGTATCAATTGCAGGTTTCTCAATTAACTCCACCGACCGCCGACCAAATG  >  1/1258077‑1258552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑