Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,254,650 G→A intergenic (+95070/+166287) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,254,6500GA98.7% 204.2 / NA 79intergenic (+95070/+166287)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (41/37);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACCTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATGCCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAGGAGAG  >  1/1254406‑1254892
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
cGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttc                                                                                                                                                                                                                                              <  1:463910/251‑1 (MQ=255)
    gACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGTAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttcagtt                                                                                                                                                                                                                                          >  1:112555/1‑251 (MQ=255)
    gACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttcagtt                                                                                                                                                                                                                                          >  1:661309/1‑251 (MQ=255)
    gACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttcagtt                                                                                                                                                                                                                                          >  1:40427/1‑251 (MQ=255)
    gACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTATACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttcagtt                                                                                                                                                                                                                                          >  1:652558/1‑251 (MQ=255)
     aCTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttcagttc                                                                                                                                                                                                                                         <  1:560991/251‑1 (MQ=255)
         gttgttgGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttcagttca                                                                                                                                                                                                                                        >  1:567793/1‑248 (MQ=255)
             ttgGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGcc                                                                                                                                                                                                                                  <  1:365610/250‑1 (MQ=255)
                                     ggCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATgg                                                                                                                                                                                                         >  1:573440/1‑251 (MQ=255)
                                                             ccGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGAtaataa                                                                                                                                                                                 <  1:610702/251‑1 (MQ=255)
                                                             ccGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGAtaataa                                                                                                                                                                                 >  1:620126/1‑251 (MQ=255)
                                                                tttCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGa                                                                                                                                                                              >  1:237017/1‑251 (MQ=255)
                                                                tttCTTTTGTGCCTGCGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGa                                                                                                                                                                              >  1:32316/1‑251 (MQ=255)
                                                                   cTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGaaa                                                                                                                                                                            >  1:43110/1‑250 (MQ=255)
                                                                   cTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAAc                                                                                                                                                                           <  1:22785/251‑1 (MQ=255)
                                                                        gtgCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttc                                                                                                                                                                                                                                              <  1:767207/179‑1 (MQ=255)
                                                                           ccTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGAtaataa                                                                                                                                                                                 <  1:365812/237‑1 (MQ=255)
                                                                              gtgCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGaaa                                                                                                                                                                >  1:82845/1‑251 (MQ=255)
                                                                               tgCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAat                                                                                                                                                               <  1:646707/251‑1 (MQ=255)
                                                                                   gcggAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCgttc                                                                                                                                                                                                                                              <  1:339351/168‑1 (MQ=255)
                                                                                   gcggAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAAc                                                                                                                                                                                                   <  1:612061/211‑1 (MQ=255)
                                                                                        aaCCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCtt                                                                                                                                                      >  1:814626/1‑251 (MQ=255)
                                                                                                             ggAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAAcc                                                                                                                                                                                                  <  1:744872/186‑1 (MQ=255)
                                                                                                              gaagaaAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACTCGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGcc                                                                                                                                                                                                                                  <  1:797683/153‑1 (MQ=255)
                                                                                                                  aaaGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTCCTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAAcgc                                                                                                                            <  1:564856/251‑1 (MQ=255)
                                                                                                                          gCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTa                                                                                                                                     >  1:3087/1‑234 (MQ=255)
                                                                                                                               tGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCg                                                                                                                  <  1:256207/248‑1 (MQ=255)
                                                                                                                                ggTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGata                                                                                                                                                                                     >  1:139499/1‑180 (MQ=255)
                                                                                                                                 gTTGATTGCGTTTTGCTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGTTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATGCCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATg                                                                                                              <  1:669512/250‑1 (MQ=255)
                                                                                                                                       tGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATag                                                                                                       <  1:175796/251‑1 (MQ=255)
                                                                                                                                       tGCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATag                                                                                                       <  1:729389/251‑1 (MQ=255)
                                                                                                                                        gCGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATag                                                                                                       <  1:159844/250‑1 (MQ=255)
                                                                                                                                         cGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATagag                                                                                                     <  1:103309/251‑1 (MQ=255)
                                                                                                                                         cGTTTTACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATagag                                                                                                     <  1:559666/251‑1 (MQ=255)
                                                                                                                                            tttACTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATc                                                                                                  <  1:75865/251‑1 (MQ=255)
                                                                                                                                                cTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGt                                                                                              >  1:226584/1‑251 (MQ=255)
                                                                                                                                                cTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGt                                                                                              >  1:146489/1‑251 (MQ=255)
                                                                                                                                                cTTCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGt                                                                                              >  1:774035/1‑251 (MQ=255)
                                                                                                                                                  tCATTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTaa                                                                                            <  1:7945/251‑1 (MQ=255)
                                                                                                                                                    aTTACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTaaaa                                                                                          >  1:302839/1‑251 (MQ=255)
                                                                                                                                                     ttACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCAtc                                                                                                                                                 <  1:90373/195‑1 (MQ=255)
                                                                                                                                                     ttACTTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCg                                                                                                                  >  1:763034/1‑226 (MQ=255)
                                                                                                                                                      tactttCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAAc                                                                                        <  1:47124/247‑1 (MQ=255)
                                                                                                                                                          ttCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATag                                                                                                       <  1:311353/232‑1 (MQ=255)
                                                                                                                                                                         ttcttcTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAgg                                                                      >  1:279823/1‑250 (MQ=255)
                                                                                                                                                                           cttctACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGaaa                                                                                                                                                                <  1:266070/158‑1 (MQ=255)
                                                                                                                                                                           cttctACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCaa                                                                   >  1:442929/1‑251 (MQ=255)
                                                                                                                                                                              ctACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGt                                                                >  1:275615/1‑251 (MQ=255)
                                                                                                                                                                                acacGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGttt                                                              >  1:660598/1‑251 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATg                                                                                                                                                                               >  1:720115/1‑129 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAatat                                                                                                                                                             <  1:507866/147‑1 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATcc                                                                                                                                        >  1:852809/1‑168 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATag                                                                                                       >  1:63168/1‑201 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTa                                                     >  1:97502/1‑251 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTa                                                     >  1:699337/1‑251 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTa                                                     >  1:419047/1‑251 (MQ=255)
                                                                                                                                                                                         ggATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTa                                                     >  1:601603/1‑251 (MQ=255)
                                                                                                                                                                                          gATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGAtaataa                                                                                                                                                                                 <  1:133013/126‑1 (MQ=255)
                                                                                                                                                                                          gATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAgaga                                                                                                    >  1:853544/1‑203 (MQ=255)
                                                                                                                                                                                                gggTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTa                                               >  1:504311/1‑250 (MQ=255)
                                                                                                                                                                                                   tGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACaa                                            <  1:417837/250‑1 (MQ=255)
                                                                                                                                                                                                                  gTTTTAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATttgtt                            >  1:52221/1‑251 (MQ=255)
                                                                                                                                                                                                                   ttttAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAacc                                                                               >  1:773461/1‑199 (MQ=255)
                                                                                                                                                                                                                   ttttAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAac                                                                                <  1:519596/198‑1 (MQ=255)
                                                                                                                                                                                                                   ttttAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATttgttg                           >  1:297775/1‑251 (MQ=255)
                                                                                                                                                                                                                   ttttAATTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATttgttg                           >  1:164395/1‑251 (MQ=255)
                                                                                                                                                                                                                        aTTCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTaa                      <  1:79429/251‑1 (MQ=255)
                                                                                                                                                                                                                         ttCCAAACGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATag                                                                                                       <  1:131349/169‑1 (MQ=255)
                                                                                                                                                                                                                                cGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATc                                                                                                                                         <  1:275494/128‑1 (MQ=255)
                                                                                                                                                                                                                                cGTCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTaa              >  1:268660/1‑251 (MQ=255)
                                                                                                                                                                                                                                  tCCGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAAt            >  1:456870/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ccGTTAACTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAAtt           >  1:488972/1‑251 (MQ=255)
                                                                                                                                                                                                                                        aaCTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAgg      <  1:840852/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAgg      <  1:52699/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCTGCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAgg      <  1:554078/251‑1 (MQ=255)
                                                                                                                                                                                                                                            gCCGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAGgagag  >  1:78333/1‑251 (MQ=255)
                                                                                                                                                                                                                                             ccGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAAc                                                                                                                                                                           <  1:456026/81‑1 (MQ=255)
                                                                                                                                                                                                                                             ccGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCAtc                                                                                                                                                 >  1:151621/1‑107 (MQ=255)
                                                                                                                                                                                                                                             ccGCAATACCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAGgag    <  1:433318/248‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
CGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACCTTCCTGCACCAAAATTTCTTCTACACGGCCGGGATAAAGGGTGGCAATGTCCAGCCGTTTTAATTCCAAACGTCCGTTAACTGCCGCAATGCCGTTCAGTTCACTGGCCTGTAATTGCCTGTTTTTCCAAATGGCGAAACCGACGGCACCGATAATAATGAAACCGAACACGAAATATTTTGCTTTCATCTCTGTATCCTTACTTTAACGCGGTTTATGCGAATGATTATAGAGATCGGGTAAAAACCACTCAACCACCAAGAGGCAACGTTTTGTTGCTTAACCCTACAAAAAGGCGTAATTTGTTGCTTAATTCGGTAAATTTAAGGAGAG  >  1/1254406‑1254892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑