Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,254,329	A→G	intergenic (+94749/+166608)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,254,329	0	A	G	100.0%	201.2 / NA	74	intergenic (+94749/+166608)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/23);  total (51/23)

ACATTGATATAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGACGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACCTTCCTGCACCAAAAT  >  1/1254084‑1254574                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aCATTGATATAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGgtg                                                                                                                                                                                                                                                  >  1:742769/1‑251 (MQ=255) aCATTGATATAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTCCGTGGTGTTCACGGCAGCGAGGGACGCCTTATAACCGGCTTGGCGgtg                                                                                                                                                                                                                                                  >  1:679723/1‑251 (MQ=255)      gATATAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGgtaggca                                                                                                                                                                                                                                              >  1:864002/1‑245 (MQ=255)       atatAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTaa                                                                                                                                                                                                                                            >  1:313329/1‑251 (MQ=255)       atatAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTaa                                                                                                                                                                                                                                            >  1:173686/1‑251 (MQ=255)         ataGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAAtt                                                                                                                                                                                                                                          <  1:422766/251‑1 (MQ=255)            ggTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAAtt                                                                                                                                                                                                                                          >  1:890138/1‑248 (MQ=255)            ggTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCg                                                                                                                                                                                                                                        >  1:457269/1‑250 (MQ=255)            ggTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCg                                                                                                                                                                                                                                        >  1:705704/1‑250 (MQ=255)                          cGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGa                                                                                                                                                                                                                         >  1:726322/1‑251 (MQ=255)                               aaaCTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTAt                                                                                                                                                                                                                     >  1:245572/1‑250 (MQ=255)                               aaaCTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTAt                                                                                                                                                                                                                     >  1:540487/1‑250 (MQ=255)                                 aCTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGa                                                                                                                                                                                                                  >  1:450635/1‑251 (MQ=255)                                 aCTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGa                                                                                                                                                                                                                  <  1:885996/251‑1 (MQ=255)                                                aCCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCGTATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTtgtg                                                                                                                                                                                                   <  1:688473/251‑1 (MQ=255)                                                                  gCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAAtt                                                                                                                                                                                 >  1:203309/1‑251 (MQ=255)                                                                   ccTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAAttt                                                                                                                                                                                <  1:280553/251‑1 (MQ=255)                                                                          ccGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACg                                                                                                                                                                         >  1:770820/1‑251 (MQ=255)                                                                                 ttGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTc                                                                                                                                                                                           >  1:159526/1‑226 (MQ=255)                                                                                     tatTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACttgttg                                                                                                                                                              <  1:501025/251‑1 (MQ=255)                                                                                         ccACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGa                                                                                                                                                                                                                  <  1:396229/195‑1 (MQ=255)                                                                                                      tttCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTc                                                                                                                                                                                      <  1:132057/210‑1 (MQ=255)                                                                                                            cgcTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGAt                                                                                                                                        <  1:324967/250‑1 (MQ=255)                                                                                                                ttAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAAtt                                                                                                                                                                                                                                          >  1:12997/1‑148 (MQ=255)                                                                                                                  aaTCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGgcg                                                                                                                                  >  1:716163/1‑250 (MQ=255)                                                                                                                       gCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTc                                                                                                                                                                                           >  1:844449/1‑188 (MQ=255)                                                                                                                        cATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGc                                                                                                                             <  1:306/249‑1 (MQ=255)                                                                                                                        cATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGc                                                                                                                             <  1:463694/249‑1 (MQ=255)                                                                                                                          tatCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACg                                                                                                                         >  1:368006/1‑251 (MQ=255)                                                                                                                            tCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTtgtg                                                                                                                                                                                                   >  1:29834/1‑175 (MQ=255)                                                                                                                            tCGGTATTTTGCGATTGCGCTTGATCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGAATATCGAGGCGCCATTTTTGTGCGTTATTCCATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGTCGCGGGCAACGGa                                                                                                                       >  1:306796/1‑251 (MQ=255)                                                                                                                             cGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGAt                                                                                                                      <  1:594539/251‑1 (MQ=255)                                                                                                                               gTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACttg                                                                                                                                                                 >  1:861902/1‑206 (MQ=255)                                                                                                                               gTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGAt                                                                                                                                        <  1:647545/231‑1 (MQ=255)                                                                                                                               gTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGAttt                                                                                                                    >  1:824631/1‑250 (MQ=255)                                                                                                                                             cgcTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGttt                                                                                                        >  1:651893/1‑249 (MQ=255)                                                                                                                                                 tGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCACTTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTtg                                                                                                  >  1:428504/1‑251 (MQ=255)                                                                                                                                                 tGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTtg                                                                                                  >  1:691411/1‑251 (MQ=255)                                                                                                                                                 tGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTtg                                                                                                  >  1:137852/1‑251 (MQ=255)                                                                                                                                                 tGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAA‑‑‑GCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGAt                                                                                                                                        >  1:251589/1‑210 (MQ=255)                                                                                                                                                  gTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGt                                                                                                                                                                        <  1:885489/180‑1 (MQ=255)                                                                                                                                                   ttCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGAt                                                                                                                                        >  1:268645/1‑211 (MQ=255)                                                                                                                                                   ttCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAggg                                                                                                                                    <  1:53139/215‑1 (MQ=255)                                                                                                                                                   ttCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTtgtg                                                                                                >  1:822345/1‑251 (MQ=255)                                                                                                                                                   ttCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTtgtg                                                                                                >  1:742751/1‑251 (MQ=255)                                                                                                                                                         cTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCtgtg                                                                                          >  1:868928/1‑251 (MQ=255)                                                                                                                                                         cTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCtgtg                                                                                          <  1:876226/251‑1 (MQ=255)                                                                                                                                                           gcgcTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACATTGACTTGTTGTTGGCGGGCATCAATTTCTGATAggg                                                                                                                                    >  1:448640/1‑207 (MQ=255)                                                                                                                                                                    ccTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTtg                                                                                                                                                                                                     <  1:672522/133‑1 (MQ=255)                                                                                                                                                                       gtgCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTg                                                                             >  1:746943/1‑250 (MQ=255)                                                                                                                                                                          cGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTgcgc                                                                          <  1:707010/250‑1 (MQ=255)                                                                                                                                                                                              gCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAg                                                     >  1:194698/1‑251 (MQ=255)                                                                                                                                                                                                tttGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGc                                                    >  1:63245/1‑250 (MQ=255)                                                                                                                                                                                                                gtgtTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGgaaga                                                        >  1:654272/1‑230 (MQ=255)                                                                                                                                                                                                                  gtTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGAt                                    >  1:285086/1‑248 (MQ=255)                                                                                                                                                                                                                  gtTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTg                                  >  1:590856/1‑250 (MQ=255)                                                                                                                                                                                                                  gtTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATGCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTTACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTTTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTg                                  >  1:769190/1‑250 (MQ=255)                                                                                                                                                                                                                         gCAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGAt                                                                                                                                        <  1:151796/141‑1 (MQ=255)                                                                                                                                                                                                                          cAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTAc                          <  1:592276/250‑1 (MQ=255)                                                                                                                                                                                                                          cAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTAc                          <  1:543983/250‑1 (MQ=255)                                                                                                                                                                                                                          cAGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTAc                          <  1:31622/250‑1 (MQ=255)                                                                                                                                                                                                                           aGCGAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTg                                  >  1:396374/1‑241 (MQ=255)                                                                                                                                                                                                                              gAGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCAt                     >  1:308311/1‑251 (MQ=255)                                                                                                                                                                                                                               aGTGACGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCAtt                    >  1:525754/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGCCTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAAt                                                                                                                                                                                        >  1:462090/1‑82 (MQ=255)                                                                                                                                                                                                                                      ccTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTActtt               <  1:337253/249‑4 (MQ=255)                                                                                                                                                                                                                                      ccTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTc              >  1:522782/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTc              >  1:728341/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTc              >  1:602460/1‑250 (MQ=255)                                                                                                                                                                                                                                       cTTATAAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGcggcgg                                                                                    >  1:541347/1‑179 (MQ=255)                                                                                                                                                                                                                                          ataAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCa         >  1:843468/1‑251 (MQ=255)                                                                                                                                                                                                                                          ataAGCGGCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCa         >  1:417264/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gCTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACttgttg                                                                                                                                                              >  1:450581/1‑95 (MQ=255)                                                                                                                                                                                                                                                  cTTGGCGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACTTTCCTGCACCAAAAt  <  1:448065/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ACATTGATATAGGTATCCGTCGGATCGAGCAAACTGACCACTTTGCCACCGGCACCGAGAACATTGCCTACTTCCGCAATTTGATATTCCACCCGTCCGTCTTTCGGCGCTTTAATCAGCATATCGGTATTTTGCGATTGCGCTTGTTCTAACTGCGCTTGCGCCTGTGCGACGGCGGCTTGTGCTTCGGCTTTGGCGGCTTGCGTGGTGTTCACGGCAGCGAGTGACGCCTTATAAGCGGCTTGACGGTGTTCTAATTCGCTGCTTGAAATCAGATTATCGAGGCGCAATTTTTGTGCGTTATTCAATTCCAATTTCGTTACGTTGACTTGTTGTTGGCGGGCATCAATTTCTGATAGGGCGCGGGCAACGGATTCTTCCGCCCGTTTCTTTTGTGCCTGTGCGGCGGAAACCTGCGCTTGCGAAATGCTGGAAGAAAGCCGTGCCAGTGGTTGATTGCGTTTTACTTCATTACCTTCCTGCACCAAAAT  >  1/1254084‑1254574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑