Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,250,574	G→A	intergenic (+90994/+170363)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,250,574	0	G	A	100.0%	187.6 / NA	69	intergenic (+90994/+170363)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/29);  total (40/29)

TGGAGCGTACGGGCATCACCAAAATATGTTCCAGCGTGCCGCGCTCCCGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCGGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTGGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGCGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGTTCGGCGGAATCAGGCTTTGTTG  >  1/1250368‑1250818                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     tGGAGCGTACGGGCATCACCAAAATATGTTCCAGCGTGCCGCGCTCCCGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCg                                                                                                                                                                                                          <  1:496330/251‑1 (MQ=255)  ggAGCGTACGGGCATCACCAAAATATGTTCCAGCGTGCCGCGCTCCCGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCgg                                                                                                                                                                                                         >  1:866219/1‑251 (MQ=255)     gCGTACGGGCATCACCAAAATATGTTCCAGCGTGCCGCGCTCCCGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGaa                                                                                                                                                                                                       >  1:686870/1‑250 (MQ=255)                          atGTTCCAGCGTGCCGCGCTCCCGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTg                                                                                                                                                                                 >  1:368723/1‑251 (MQ=255)                                              cccGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAAc                                                                                                                                                             <  1:891899/251‑1 (MQ=255)                                              cccGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAAc                                                                                                                                                             <  1:412280/251‑1 (MQ=255)                                                        aaTGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGATATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCg                                                                                                                                                   >  1:688652/1‑251 (MQ=255)                                                                         ccaGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGt                                                                                                                                  >  1:760814/1‑251 (MQ=255)                                                                         ccaGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGt                                                                                                                                  >  1:604460/1‑251 (MQ=255)                                                                            gCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACa                                                                                                                               >  1:128838/1‑251 (MQ=255)                                                                                   ggTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGtt                                                                                                                        >  1:837937/1‑251 (MQ=255)                                                                                   ggTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGtt                                                                                                                        >  1:468899/1‑251 (MQ=255)                                                                                    gTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGtt                                                                                                                        >  1:855194/1‑250 (MQ=255)                                                                                    gTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGtt                                                                                                                        >  1:280815/1‑250 (MQ=255)                                                                                        ataaATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATaa                                                                                                                   <  1:560/251‑1 (MQ=255)                                                                                            aTTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGc                                                                                                                                                                                                           <  1:780674/159‑1 (MQ=255)                                                                                               cAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACg                                                                                                                          >  1:651870/1‑237 (MQ=255)                                                                                                       ccACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACg                                                                                                                          <  1:383334/229‑1 (MQ=255)                                                                                                            aTGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAAt                                                                                               <  1:268483/251‑1 (MQ=255)                                                                                                            aTGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAAt                                                                                               >  1:764495/1‑251 (MQ=255)                                                                                                            aTGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAAt                                                                                               <  1:472676/251‑1 (MQ=255)                                                                                                             tGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATaa                                                                                                                   <  1:129695/230‑1 (MQ=255)                                                                                                             tGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATg                                                                                              >  1:112702/1‑251 (MQ=255)                                                                                                             tGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATg                                                                                              >  1:800254/1‑251 (MQ=255)                                                                                                              gTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGa                                                                                             >  1:187706/1‑251 (MQ=255)                                                                                                              gTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGa                                                                                             >  1:629203/1‑251 (MQ=255)                                                                                                                        cccATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGc                                                                                                                                           >  1:792106/1‑195 (MQ=255)                                                                                                                        cccATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATAtt                                                                                    >  1:315666/1‑250 (MQ=255)                                                                                                                        cccATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATAtt                                                                                    >  1:498288/1‑250 (MQ=255)                                                                                                                        cccATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATtc                                                                                   <  1:650771/251‑1 (MQ=255)                                                                                                                           aTAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTcc                                                                                <  1:32574/251‑1 (MQ=255)                                                                                                                             aaaCCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCtg                                                                              <  1:726217/251‑1 (MQ=255)                                                                                                                                ccAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTgtgt                                                                           <  1:41641/251‑1 (MQ=255)                                                                                                                                ccAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTgtgt                                                                           <  1:786421/251‑1 (MQ=255)                                                                                                                                ccAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTgtgt                                                                           <  1:591876/251‑1 (MQ=255)                                                                                                                                   gTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCa                                                                        >  1:104594/1‑251 (MQ=255)                                                                                                                                     tACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATc                                                                      <  1:819625/251‑1 (MQ=255)                                                                                                                                          gAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGtat                                                                                       <  1:770648/229‑1 (MQ=255)                                                                                                                                                    ggTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCtg                                                        <  1:808992/250‑1 (MQ=255)                                                                                                                                                        ataAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTatat                                                                                     >  1:666591/1‑217 (MQ=255)                                                                                                                                                             gCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGa                                              >  1:84154/1‑251 (MQ=255)                                                                                                                                                                caTTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATCGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAAtt                                           >  1:145630/1‑251 (MQ=255)                                                                                                                                                                  ttAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTg                                          >  1:737075/1‑250 (MQ=255)                                                                                                                                                                  ttAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGCGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGt                                         >  1:157977/1‑251 (MQ=255)                                                                                                                                                                         tggGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCAc                                   <  1:38761/250‑1 (MQ=255)                                                                                                                                                                         tggGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCAc                                   <  1:7486/250‑1 (MQ=255)                                                                                                                                                                          gggTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAAt                                                                                               <  1:289409/189‑1 (MQ=255)                                                                                                                                                                           ggTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTcc                                                                                >  1:585140/1‑203 (MQ=255)                                                                                                                                                                           ggTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAgg                                                                   <  1:379171/216‑1 (MQ=255)                                                                                                                                                                           ggTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCAt                                                <  1:150973/235‑1 (MQ=255)                                                                                                                                                                           ggTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACttt                                >  1:317951/1‑251 (MQ=255)                                                                                                                                                                            gTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAAt                                                                                                                                                                                                                                         >  1:564400/1‑49 (MQ=255)                                                                                                                                                                            gTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGaa                                             >  1:133334/1‑237 (MQ=255)                                                                                                                                                                            gTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCAc                                   <  1:456730/247‑1 (MQ=255)                                                                                                                                                                               gAATGAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCAc                                   >  1:687576/1‑244 (MQ=255)                                                                                                                                                                                   gAGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTgtgt                                                                           >  1:326392/1‑200 (MQ=255)                                                                                                                                                                                    aGTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGtt                       >  1:844904/1‑251 (MQ=255)                                                                                                                                                                                     gTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAAc                                                                                                                                                             <  1:760517/116‑1 (MQ=255)                                                                                                                                                                                     gTATGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCAc                                                                                                                           >  1:462951/1‑150 (MQ=255)                                                                                                                                                                                        tGCCGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGa                           <  1:239688/243‑1 (MQ=255)                                                                                                                                                                                          ccGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTaaaa                                                                                                >  1:374735/1‑172 (MQ=255)                                                                                                                                                                                          ccGTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACt                                  >  1:811215/1‑234 (MQ=255)                                                                                                                                                                                            gTTATTGCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGTTCGGCGGaa               >  1:131833/1‑251 (MQ=255)                                                                                                                                                                                                  gCTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGTTCGGCGGAATCAgg          >  1:168582/1‑250 (MQ=255)                                                                                                                                                                                                   cTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATg                                                                                              <  1:565764/165‑1 (MQ=255)                                                                                                                                                                                                   cTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATAtt                                                                                    <  1:525723/175‑1 (MQ=255)                                                                                                                                                                                                   cTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGa                           >  1:771660/1‑232 (MQ=255)                                                                                                                                                                                                   cTTTGAAGGTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGTTCGGCGGAATCAGGc         <  1:673450/250‑1 (MQ=255)                                                                                                                                                                                                           gTGCAGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTCGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGTGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGTTCGGCGGAATCAGGCTttgttg  >  1:670326/1‑249 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     TGGAGCGTACGGGCATCACCAAAATATGTTCCAGCGTGCCGCGCTCCCGTTCCCGAATGACAGCGGCGCCCACCAGCAACATGGTTAATAAATTCAAATAGCCCACAATGTTCATGCCGCCCATAAACCAGTTACCGGAAAAATTCGGGTTATAAAGCACATTAATGGTGGGTTGAATGAGTATGCCGTTATTGCTTTGAAGGTGCGGAAAATCGTTAATTTCACCGTGAAAAATTTGTGAAATATAAGCGGAACCGATGGCGGCTTGCGTCATTGCCGTGGCGTCGGATAATAACTGAATCAGCGGGTTACGCCCCGCAAGTACATCACGTTCATAATTCGGCGGAATATCTAAAATGAACGTATATTCTCCTGTGTCCATCAGGCGATCCGCCTGTTGTGCATGAATTGTATGCACTTTACGGAAGTTCGGCGGAATCAGGCTTTGTTG  >  1/1250368‑1250818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑