Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,248,722	G→A	intergenic (+89142/+172215)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,248,722	0	G	A	100.0%	144.4 / NA	55	intergenic (+89142/+172215)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/8);  total (47/8)

CCTAGTGCTTTATACAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGGCGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGTATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGGCTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTATTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAATCCGCTTTGGCAGAAGCCAGT  >  1/1248476‑1248955                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ccTAGTGCTTTATACAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTg                                                                                                                                                                                                                                       >  1:737988/1‑251 (MQ=255)      tGCTTTATACAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTgcag                                                                                                                                                                                                                                    <  1:772419/249‑1 (MQ=255)       gCTTTATACAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCaa                                                                                                                                                                                                                                 >  1:727317/1‑251 (MQ=255)       gCTTTATACAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGGTATCTACATCGGCAAGGACTTGCAGCaa                                                                                                                                                                                                                                 >  1:445219/1‑251 (MQ=255)            ataCAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCtt                                                                                                                                                                                                                            >  1:126934/1‑251 (MQ=255)            ataCAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCtt                                                                                                                                                                                                                            >  1:721070/1‑251 (MQ=255)            ataCAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCtt                                                                                                                                                                                                                            >  1:272062/1‑251 (MQ=255)                     aaTCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTAtt                                                                                                                                                                                                                   >  1:178532/1‑251 (MQ=255)                       tCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGa                                                                                                                                                                                                                 >  1:406841/1‑251 (MQ=255)                         aaaTTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAg                                                                                                                                                                                                               <  1:785036/251‑1 (MQ=255)                               ttcGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGc                                                                                                                                                                                                         <  1:669854/251‑1 (MQ=255)                                         cTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAgg                                                                                                                                                                                               <  1:66295/251‑1 (MQ=255)                                                gCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAg                                                                                                                                                                                                               >  1:492226/1‑228 (MQ=255)                                                 cTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGt                                                                                                                                                                                        >  1:402007/1‑250 (MQ=255)                                                     ggATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTcggcg                                                                                                                                                                                   >  1:66912/1‑251 (MQ=255)                                                           gTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGt                                                                                                                                                                                                              <  1:774988/218‑1 (MQ=255)                                                              ggTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGGCAATg                                                                                                                                                                          >  1:327917/1‑251 (MQ=255)                                                               gTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGt                                                                                                                                                                         >  1:783760/1‑251 (MQ=255)                                                                   tGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAgg                                                                                                                                                                                               <  1:83448/225‑1 (MQ=255)                                                                   tGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGGCGGCGGCGTCAATGTTggg                                                                                                                                                                     >  1:132353/1‑250 (MQ=255)                                                                   tGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGGAAGGCAGCTTTCAGGCGGGCGGCGGGGGCGTCAATGTTggg                                                                                                                                                                     >  1:120291/1‑250 (MQ=255)                                                                     gTAATCCAGTGCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTgcag                                                                                                                                                                                                                                    >  1:471006/1‑186 (MQ=255)                                                                               gCGGTGAGGCGGGCGGTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCCGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTc                                                                                                                                                          >  1:49252/1‑250 (MQ=255)                                                                                              gTGAGGACGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGt                                                                                                                                                                                                              <  1:401820/183‑1 (MQ=255)                                                                                                   gacGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAgg                                                                                                                                      >  1:226484/3‑250 (MQ=255)                                                                                                        tGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATg                                                                                                                                >  1:347028/1‑251 (MQ=255)                                                                                                         gTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGt                                                                                                                               >  1:401574/1‑251 (MQ=255)                                                                                                                              gTTAAATAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGccc                                                                                                           >  1:502796/1‑250 (MQ=255)                                                                                                                                 aaaTAATTTCTCCGCATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTcaacaa                                                                                                                  >  1:462303/1‑240 (MQ=255)                                                                                                                                             cgcaTTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGt                                                                                                                                                                         >  1:316781/4‑173 (MQ=255)                                                                                                                                             cgcaTTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCgg                                                                                           >  1:11560/4‑251 (MQ=255)                                                                                                                                                    cctgcctgCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGt                                                                                     >  1:641742/1‑250 (MQ=255)                                                                                                                                                      tgcctgcTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCCGCTTTCAGGCGGGTGTCGGTGGCGTCTATGTTGGCTTGAATGCGTCCGTTAGTGAACATCGGCAGGCCAATGTCGAGGCCCCACAAATTGACCCAACCCTTTAAATCCGGAATGTCCg                                                                                  >  1:840557/1‑251 (MQ=255)                                                                                                                                                       gcctgcTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGt                                                                                                                               >  1:657373/1‑205 (MQ=255)                                                                                                                                                                      tGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGt                                                                                                                                                                         >  1:147234/1‑148 (MQ=255)                                                                                                                                                                                  gTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTg                                                                             >  1:229047/1‑228 (MQ=255)                                                                                                                                                                                  gTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTc                                                       >  1:174529/1‑250 (MQ=255)                                                                                                                                                                                         gCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCt                                                                                                                                    >  1:772407/1‑166 (MQ=255)                                                                                                                                                                                                         aTTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGccc                                                                                                           >  1:617917/1‑175 (MQ=255)                                                                                                                                                                                                         aTTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAgcg                                 >  1:263495/1‑249 (MQ=255)                                                                                                                                                                                                         aTTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGcgcg                               >  1:584252/1‑251 (MQ=255)                                                                                                                                                                                                              aTGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGtat                          <  1:370634/251‑1 (MQ=255)                                                                                                                                                                                                                   gCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAAACGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAAt                      >  1:115444/1‑250 (MQ=255)                                                                                                                                                                                                                       gtgCCTGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGc                                                                                                                                                                                                         >  1:712845/1‑67 (MQ=255)                                                                                                                                                                                                                            tGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCgtgt                                                                               >  1:824816/1‑184 (MQ=255)                                                                                                                                                                                                                            tGATAGCTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAATCCGCTTTgg             >  1:176992/1‑250 (MQ=255)                                                                                                                                                                                                                                  cTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTc                                                                                    >  1:271539/1‑173 (MQ=255)                                                                                                                                                                                                                                  cTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAATCCGCTTTGGCAGAAg       >  1:160731/1‑250 (MQ=255)                                                                                                                                                                                                                                  cTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAATCCGCTTTGGCAGAAg       >  1:811764/1‑250 (MQ=255)                                                                                                                                                                                                                                  cTGTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTACAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCCAATGTCGAGGCTCAACAAAATGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGGATAAATCCGCTTTGGCAGAAg       >  1:370364/1‑250 (MQ=255)                                                                                                                                                                                                                                    gTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAAt                                                                                                                                                               >  1:450255/1‑96 (MQ=255)                                                                                                                                                                                                                                    gTTATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAAt                      >  1:298790/1‑233 (MQ=255)                                                                                                                                                                                                                                      tATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTc                                                                        >  1:443617/1‑181 (MQ=255)                                                                                                                                                                                                                                      tATCTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTGTTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAATCCGCTTTGGCAGAAGCCAGt  >  1:372997/1‑251 (MQ=255)                                                                                                                                                                                                                                         cTACATCGGCAAGGACTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGtt                                                                                                                                                      >  1:281196/1‑100 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CCTAGTGCTTTATACAAACTAATCAAATTCTTCGCCCCCGCTAAACGGCTTTGGATGAGTTGGTTTTGGTAATCCAGTGCGGTGAGGCGGGCGGTGAGGGCGTTGTCTAAGGTTTTTTCGCCGTAGTTAAATAATTTCTCCGTATTGCCTGCCTGCTTTTGCGCCTGTTGGCAGGCGGTTTGTAGCAATTGGGTTTGGCGATTTAATGCGGCTTGTGCCTGATAGCTGTTATCTACATCGGCAAGGGCTTGCAGCAAGCCTTTGTCGTATTGAAGTAAGGCAGCTTTCAGGCGGGCGTCGGCGGCGTCAATGTTGGCTTGAATGCGTCCGTTAGTGAAAATCGGCAGGCTAATGTCGAGGCTCAACAAATTGCCCCAACCTTTTAAATCGGGAATGTCCGTGTTGATGTCAATGCGCCCTATTTGTCCTAAAAATTGAATCTCAAAGCGCGGGTATAAATCCGCTTTGGCAGAAGCCAGT  >  1/1248476‑1248955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑