Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,231,844	G→A	intergenic (+72264/+189093)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,231,844	0	G	A	100.0%	187.7 / NA	69	intergenic (+72264/+189093)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/29);  total (40/29)

TGTCTATGAAAAATAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTGCAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCAAAAATTAACGAAGCCAAACAAG  >  1/1231598‑1232088                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      tGTCTATGAAAAATAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAgg                                                                                                                                                                                                                                                   <  1:650515/251‑1 (MQ=255)        gAAAAATAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCAttttt                                                                                                                                                                                                                                            >  1:54016/1‑251 (MQ=255)         aaaaaTAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCAtttttt                                                                                                                                                                                                                                           >  1:247664/1‑251 (MQ=255)         aaaaaTAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGAGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGCATCCTTTTCTACTCACGAATAATTTGCGTAAAACGAGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCAtttttt                                                                                                                                                                                                                                           >  1:372235/1‑251 (MQ=255)          aaaaTAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCAttttttt                                                                                                                                                                                                                                          >  1:589363/1‑251 (MQ=255)           aaaTAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTa                                                                                                                                                                                                                                         >  1:789652/1‑251 (MQ=255)                gCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAgg                                                                                                                                                                                                                                                   >  1:726129/1‑236 (MQ=255)                   tAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAAt                                                                                                                                                                                                                                  >  1:370322/1‑250 (MQ=255)                       aGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGaa                                                                                                                                                                                                                              >  1:573328/1‑250 (MQ=255)                         aTTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACAt                                                                                                                                                                                                                           >  1:792888/1‑251 (MQ=255)                         aTTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACAt                                                                                                                                                                                                                           >  1:58366/1‑251 (MQ=255)                              gCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACag                                                                                                                                                                                                                      >  1:587724/1‑251 (MQ=255)                               cACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACaga                                                                                                                                                                                                                     <  1:336508/251‑1 (MQ=255)                                 cTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAgg                                                                                                                                                                                                                   >  1:186725/1‑251 (MQ=255)                                 cTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAgg                                                                                                                                                                                                                   >  1:66279/1‑251 (MQ=255)                                     aaTTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGAc                                                                                                                                                                                                                        <  1:783889/242‑1 (MQ=255)                                         tCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatatat                                                                                                                                                                                                           <  1:495392/251‑1 (MQ=255)                                           cTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATCTgc                                                                                                                                                                                                         <  1:462635/251‑1 (MQ=255)                                           cTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATgc                                                                                                                                                                                                         <  1:315527/251‑1 (MQ=255)                                           cTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATgc                                                                                                                                                                                                         <  1:771457/251‑1 (MQ=255)                                                ggACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAg                                                                                                                                                                                                                                                    >  1:662979/1‑203 (MQ=255)                                                ggACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatat                                                                                                                                                                                                             <  1:216074/242‑1 (MQ=255)                                                ggACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCAt                                                                                                                                                                                                    >  1:615186/1‑251 (MQ=255)                                                 gACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCa                                                                                                                                                                                                                                                 <  1:297307/205‑1 (MQ=255)                                                  aCAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatata                                                                                                                                                                                                            <  1:270518/241‑1 (MQ=255)                                                   cAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatata                                                                                                                                                                                                            <  1:694711/240‑1 (MQ=255)                                                   cAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCtt                                                                                                                                                                                                 >  1:637981/1‑251 (MQ=255)                                                      aaaGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatata                                                                                                                                                                                                            >  1:543495/1‑237 (MQ=255)                                                                cacaAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatata                                                                                                                                                                                                            >  1:369345/1‑227 (MQ=255)                                                                  caAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATgc                                                                                                                                                                                                         >  1:316133/1‑228 (MQ=255)                                                                  caAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATgc                                                                                                                                                                                                         <  1:363261/228‑1 (MQ=255)                                                                  caAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGgag                                                                                                                                                                                  >  1:482771/1‑251 (MQ=255)                                                                  caAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGgag                                                                                                                                                                                  <  1:333915/251‑1 (MQ=255)                                                                               tttttGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAg                                                                                                                                                                                             >  1:79790/1‑227 (MQ=255)                                                                                       cGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGAc                                                                                                                                                             <  1:18137/251‑1 (MQ=255)                                                                                           ggCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTa                                                                                                                                                         >  1:791388/1‑251 (MQ=255)                                                                                             cTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTAtt                                                                                                                                                       >  1:141308/1‑251 (MQ=255)                                                                                                 tCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGaaa                                                                                                                                                   <  1:558583/251‑1 (MQ=255)                                                                                                  ccAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGaaaa                                                                                                                                                  <  1:53535/251‑1 (MQ=255)                                                                                                           cGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTaa                                                                                                                                                                                               >  1:557321/1‑197 (MQ=255)                                                                                                                 gggTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTggcgg                                                                                                                                    >  1:374211/1‑250 (MQ=255)                                                                                                                  ggTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAg                                                                                                                                                               <  1:88954/222‑1 (MQ=255)                                                                                                                       agaaACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCAttttttt                                                                                                                                                                                                                                          <  1:178189/142‑1 (MQ=255)                                                                                                                       agaaACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatata                                                                                                                                                                                                            >  1:200196/1‑172 (MQ=255)                                                                                                                       agaaACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATgc                                                                                                                                                                                                         >  1:770627/1‑175 (MQ=255)                                                                                                                       agaaACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGaaa                                                                                                                                                                               >  1:679101/1‑201 (MQ=255)                                                                                                                       agaaACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTtga                                                                                                                             >  1:349602/1‑251 (MQ=255)                                                                                                                                             aaaTAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑tttt                                                                                                      <  1:832954/251‑1 (MQ=255)                                                                                                                                                                   ttATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTAccc                                                                                <  1:144470/251‑1 (MQ=255)                                                                                                                                                                      tCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGAc                                                                                                                                                             >  1:809423/1‑172 (MQ=255)                                                                                                                                                                      tCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAAt                                                                             >  1:289657/1‑251 (MQ=255)                                                                                                                                                                         gTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCAttttttt                                                                                                                                                                                                                                          <  1:774718/92‑1 (MQ=255)                                                                                                                                                                         gTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGatatata                                                                                                                                                                                                            <  1:727349/122‑1 (MQ=255)                                                                                                                                                                         gTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTaa                                                                          >  1:584780/1‑251 (MQ=255)                                                                                                                                                                           aTCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGAc                                                                                                                                                                                                                        >  1:736031/1‑108 (MQ=255)                                                                                                                                                                              cTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGAt                                                                                                                                                                                                                                      <  1:232728/91‑1 (MQ=255)                                                                                                                                                                                  tCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAAc                                                                 <  1:202493/251‑1 (MQ=255)                                                                                                                                                                                       tCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGc                                                            <  1:628910/251‑1 (MQ=255)                                                                                                                                                                                          cGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAg                                                                                                                                                               <  1:340156/150‑1 (MQ=255)                                                                                                                                                                                              taatTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAg                                                                                                                                                               <  1:222594/146‑1 (MQ=255)                                                                                                                                                                                                         aaaCGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAagag                                          <  1:200448/251‑1 (MQ=255)                                                                                                                                                                                                                     ttGCGTGATTTTCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATacag                                                                                                                                                                                                       >  1:73240/1‑83 (MQ=255)                                                                                                                                                                                                                                tCTAAGCAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATATTTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCaaaaa                    <  1:136832/251‑1 (MQ=255)                                                                                                                                                                                                                                      cAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCaaaaa                    >  1:304064/1‑244 (MQ=255)                                                                                                                                                                                                                                      cAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCAAAAATTAACGa             >  1:39676/1‑251 (MQ=255)                                                                                                                                                                                                                                      cAGTTAATATTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCAAAAATTAACGa             >  1:469405/1‑251 (MQ=255)                                                                                                                                                                                                                                            atatTATCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGc                                                                                                                                           >  1:590556/1‑120 (MQ=255)                                                                                                                                                                                                                                                tatCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCAAAAATTAACGAAGCCAAACaa   >  1:767468/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tCATTACAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCAAAAATTAACGAAGCCAAACAAg  >  1:818119/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TGTCTATGAAAAATAGCTTAATAGATTCAGCACTAAAATTTCCTGCAGGACAAAAAGTGCGGTCACAAAAAACAACGTTTTTTGAACGTTGGCTTTTCCAACCGCCCGTTAGGGGTGAAGAAACGAGAGAGCGAGTTTGTAAATAATTTTTTCGGGCGTTTTTTATCGGTATCCTTTTCTACTCACGAATAATTTGCGTAAAACGTGATAAATTGCGTGATTTTCTAAGCAGTTAATATTATCATTGCAGGCATTTTTTTAGATAAATCGAACATGACAGAGGATATATATGCAGCATCTTAAAGATCTTGTGGAGAAAGCAAGAAATGCTATAGACAGTATTGAAAATAAAAGCCTGGCGGCACTTGATGAAATTCGGGTGGAATA‑TTTTGGTAAAAAAGGGCATTTTACCCAATTAATGCAAGAACTACGCAATGTGGCGGCGGAAGAGCGTCCGGCGGTGGGTGCAAAAATTAACGAAGCCAAACAAG  >  1/1231598‑1232088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑