Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,221,847	G→A	intergenic (+62267/+199090)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,221,847	0	G	A	100.0%	115.3 / NA	44	intergenic (+62267/+199090)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/18);  total (26/18)

GAATGGGGGCGTTGCTTCTTGCAGCAGGTATAGCAACAGGTGGTATATTCGGTTCCATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCGTAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAGATTAATGAAATATTGATGTCGCGTTGAACGCCACACACGTCCACGG  >  1/1221600‑1222089                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gAATGGGGGCGTTGCTTCTTGCAGCAGGTATAGCAACAGGTGGTATATTCGGTTCCATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATaa                                                                                                                                                                                                                                                 >  1:106402/1‑251 (MQ=255)                          agGTATAGCAACAGGTGGTATATTCGGTTCCATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAAc                                                                                                                                                                                                                         >  1:492367/1‑250 (MQ=255)                            gTATAGCAACAGGTGGTATATTCGGTTCCATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTa                                                                                                                                                                                                                      >  1:877169/1‑251 (MQ=255)                                aGCAACAGGTGGTATATTCGGTTCCATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGGTGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTAccc                                                                                                                                                                                                                   >  1:179818/1‑250 (MQ=255)                                                        cATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAAc                                                                                                                                                                                                                         >  1:711695/1‑220 (MQ=255)                                                        cATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGcc                                                                                                                                                                                           >  1:303138/1‑250 (MQ=255)                                                                   ctgcAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGtt                                                                                                                                                                                <  1:893920/250‑1 (MQ=255)                                                                   ctgcAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTc                                                                                                                                                                               <  1:263799/251‑1 (MQ=255)                                                                                         ttCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAAccc                                                                                                                                                                                                       >  1:786182/1‑205 (MQ=255)                                                                                              ggATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGctt                                                                                                                                                     >  1:382356/1‑250 (MQ=255)                                                                                                    ttGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGgt                                                                                                                                              >  1:741083/1‑251 (MQ=255)                                                                                                        gAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTc                                                                                                                                                                                                                            >  1:149068/1‑169 (MQ=255)                                                                                                          gTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTggg                                                                                                                                               >  1:562879/1‑244 (MQ=255)                                                                                                             cGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAAt                                                                                                                                                                                               >  1:177187/1‑193 (MQ=255)                                                                                                                   ttGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGcttcttc                                                                                                                               <  1:658728/251‑1 (MQ=255)                                                                                                                           ttGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCga                                                                                                                       <  1:282705/251‑1 (MQ=255)                                                                                                                             ggCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTc                                                                                                                                                                               <  1:341358/193‑1 (MQ=255)                                                                                                                                                 gAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCaa                                                                                                  >  1:873787/1‑250 (MQ=255)                                                                                                                                                        aTAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGAc                                                                                           <  1:42121/250‑1 (MQ=255)                                                                                                                                                                   aTACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGgtt                                                                                                                                             >  1:209909/1‑189 (MQ=255)                                                                                                                                                                     aCGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTg                                                                              >  1:803079/1‑250 (MQ=255)                                                                                                                                                                          aTTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAgg                                                                        <  1:510191/251‑1 (MQ=255)                                                                                                                                                                          aTTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAgg                                                                        >  1:376298/1‑251 (MQ=255)                                                                                                                                                                                 tttCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGc                                                                                                          <  1:369809/210‑1 (MQ=255)                                                                                                                                                                                       aaGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGgt                                                           <  1:375219/251‑1 (MQ=255)                                                                                                                                                                                         gTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTg                                                                                                                                                 >  1:794979/1‑163 (MQ=255)                                                                                                                                                                                         gTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGgtcg                                                         >  1:876225/1‑251 (MQ=255)                                                                                                                                                                                                   aTGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAg                                                <  1:526610/250‑1 (MQ=255)                                                                                                                                                                                                   aTGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAg                                                <  1:643766/250‑1 (MQ=255)                                                                                                                                                                                                         gtgtATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGAc                                                                                                                                                                       <  1:256361/125‑1 (MQ=255)                                                                                                                                                                                                         gtgtATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGgt                                                                                                                                              <  1:517407/150‑1 (MQ=255)                                                                                                                                                                                                           gtATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGAc                                                                                     <  1:636580/205‑1 (MQ=255)                                                                                                                                                                                                           gtATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAGATTAATGaa                                       >  1:143366/1‑251 (MQ=255)                                                                                                                                                                                                           gtATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAGATTAATGaa                                       >  1:844092/1‑251 (MQ=255)                                                                                                                                                                                                               aaaTTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAGATTAATGAAatat                                   <  1:392404/251‑1 (MQ=255)                                                                                                                                                                                                                     gTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACg                                                                                                >  1:595464/1‑184 (MQ=255)                                                                                                                                                                                                                     gTCTTCCATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGAc                                                                                           >  1:87456/1‑189 (MQ=255)                                                                                                                                                                                                                          ccATTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAgg                                                                        >  1:756787/1‑203 (MQ=255)                                                                                                                                                                                                                            aTTTATTATTTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGc                                                                                                          >  1:733168/1‑167 (MQ=255)                                                                                                                                                                                                                                   attTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGc                                                                                                                                                       <  1:490416/115‑1 (MQ=255)                                                                                                                                                                                                                                   attTGAAATTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGgt                                                           <  1:25166/207‑1 (MQ=255)                                                                                                                                                                                                                                        aaaTTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCt                                                                                                                                                                                                                       >  1:318853/1‑46 (MQ=255)                                                                                                                                                                                                                                        aaaTTATAATTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGgt                                                           >  1:171138/1‑202 (MQ=255)                                                                                                                                                                                                                                                aTTTATTCATAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAGATTAATGAAATATTGATGTCGCGTTGAACGCCACACACGTCCACgg  <  1:869686/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GAATGGGGGCGTTGCTTCTTGCAGCAGGTATAGCAACAGGTGGTATATTCGGTTCCATTTATGCTGCTGCAAATGGATATGAAAAGCTTTCTTGGATCATTGCGAGTACGTGTATTGGTTCATTGGCGATCGCTTTTCTAAAAAGAAATAGATAAAAATATGATACGTAATTTTTATTTCTGAAGTAATGGAAGATGTAAGTGTATAAATTTGTCTTCCATTTATTATTTGAAATTATAATTTATTCGTAAAAACCAACCGCACTTTTCCTCAACCTACCCCTGATTCAACCCCAAAAAATCGCCCACTTCATGTTCCAACTGACTCATGGCGATCAAAGCTTCTTGGGTTTGTTTGGCTTCTTCTTCGTCGATGATGCTCATGGCGAAGCCAACGTGGACGAGGACCCATTTGCCAAGGAGCAGTTTGGGGTCGTCGTTGCAGATTAATGAAATATTGATGTCGCGTTGAACGCCACACACGTCCACGG  >  1/1221600‑1222089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑