Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,220,968 T→G intergenic (+61388/+199969) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,220,9680TG98.6% 196.9 / ‑3.1 73intergenic (+61388/+199969)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (31/41);  minor base . (0/1);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

AATATTTATTGATAAGTTTCGACATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGTTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCAGGGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGACTTGGAGGTTATATATG  >  1/1220723‑1221213
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
aaTATTTATTGATAAGTTTCGACATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGgttttt                                                                                                                                                                                                                                                  >  1:746506/1‑251 (MQ=255)
       attGATAAGTTTCGACATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATg                                                                                                                                                                                                                                           >  1:522842/1‑251 (MQ=255)
               gTTTCGACATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTAc                                                                                                                                                                                                                                               <  1:312722/239‑1 (MQ=255)
                     aCATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAAc                                                                                                                                                                                                                                 <  1:703426/247‑1 (MQ=255)
                     aCATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGAt                                                                                                                                                                                                                              <  1:6898/250‑1 (MQ=255)
                     aCATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATa                                                                                                                                                                                                                             >  1:605063/1‑251 (MQ=255)
                                aTTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCg                                                                                                                                                                                                                  <  1:397648/251‑1 (MQ=255)
                                       aaaTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCacac                                                                                                                                                                                                           <  1:857645/251‑1 (MQ=255)
                                       aaaTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCacac                                                                                                                                                                                                           <  1:357910/251‑1 (MQ=255)
                                       aaaTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTG‑GTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACt                                                                                                                                                                                                          <  1:555550/251‑1 (MQ=255)
                                        aaTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACt                                                                                                                                                                                                          <  1:389581/251‑1 (MQ=255)
                                        aaTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACt                                                                                                                                                                                                          <  1:605989/251‑1 (MQ=255)
                                        aaTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACt                                                                                                                                                                                                          <  1:800663/251‑1 (MQ=255)
                                         aTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAg                                                                                                                                                                                                                    >  1:20657/1‑240 (MQ=255)
                                         aTCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCg                                                                                                                                                                                                                  >  1:327191/1‑242 (MQ=255)
                                               aTGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTg                                                                                                                                                                                                   <  1:57396/251‑1 (MQ=255)
                                               aTGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTg                                                                                                                                                                                                   <  1:344914/251‑1 (MQ=255)
                                                          gACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTa                                                                                                                                                                                                                                                <  1:282422/195‑1 (MQ=255)
                                                           aCCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAatatatat                                                                                                                                                                                        <  1:83809/250‑1 (MQ=255)
                                                               acacAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTa                                                                                                                                                                                   >  1:791692/1‑251 (MQ=255)
                                                                cacaAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCacac                                                                                                                                                                                                           <  1:303789/226‑1 (MQ=255)
                                                                cacaAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGc                                                                                                                                                                                                     >  1:330989/1‑232 (MQ=255)
                                                                cacaAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGc                                                                                                                                                                                                     <  1:130038/232‑1 (MQ=255)
                                                                      aCCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATGAAAACCGTGATTGAGATCATAATGTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCGCACTTGTGCTGAAAatatata                                                                                                                                                                                         <  1:846039/238‑1 (MQ=255)
                                                                                          gATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCacac                                                                                                                                                                                                           <  1:706860/200‑1 (MQ=255)
                                                                                          gATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACt                                                                                                                                                                                                          >  1:140895/1‑201 (MQ=255)
                                                                                              aaTTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTg                                                                                                                                                    >  1:79747/1‑251 (MQ=255)
                                                                                               aTTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGC‑TGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTg                                                                                                                                                                                                   <  1:774586/202‑1 (MQ=255)
                                                                                                       aCCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAatatata                                                                                                                                                                                         <  1:553948/205‑1 (MQ=255)
                                                                                                          gAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTAc                                                                                                                                                                                                                                               <  1:709705/148‑1 (MQ=255)
                                                                                                               gTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTg                                                                                                                                                               <  1:86858/223‑1 (MQ=255)
                                                                                                                  cTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGAt                                                                                                                                                                                                                              <  1:281959/157‑1 (MQ=255)
                                                                                                                  cTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGAt                                                                                                                                                                                                                              <  1:569088/157‑1 (MQ=255)
                                                                                                                                 gTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAAtt                                                                                                                >  1:638546/1‑251 (MQ=255)
                                                                                                                                   cGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAAc                                                                                                                                                         >  1:597484/1‑209 (MQ=255)
                                                                                                                                   cGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTa                                                                                                               >  1:666242/1‑250 (MQ=255)
                                                                                                                                   cGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTAc                                                                                                              <  1:197392/251‑1 (MQ=255)
                                                                                                                                               tttttGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAatatat                                                                                                  <  1:36883/251‑1 (MQ=255)
                                                                                                                                               tttttGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAatatat                                                                                                  <  1:766417/251‑1 (MQ=255)
                                                                                                                                                           gAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATaaaaaa                                                                                        >  1:71253/1‑249 (MQ=255)
                                                                                                                                                             gTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGaaaaa                                                                                                                                                          <  1:614410/182‑1 (MQ=255)
                                                                                                                                                              tttCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGaaa                                                                                                                                                            >  1:160356/1‑179 (MQ=255)
                                                                                                                                                                   ttCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCacac                                                                                                                                                                                                           <  1:728622/127‑1 (MQ=255)
                                                                                                                                                                       aGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAAttt                                                                          >  1:289450/1‑251 (MQ=255)
                                                                                                                                                                         cGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAAttt                                                                          <  1:346012/249‑1 (MQ=255)
                                                                                                                                                                         cGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTGTTTACGATGAATTTTAAACGATAGCGCTCAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAAttt                                                                          <  1:450298/249‑1 (MQ=255)
                                                                                                                                                                           gATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTcagcag                                                                                                                                           <  1:321303/183‑1 (MQ=255)
                                                                                                                                                                                 cTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACg                                                                                                                                                                                                                                <  1:819151/92‑1 (MQ=255)
                                                                                                                                                                                     aTCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGc                                                                                                                                                                                                     >  1:552596/1‑115 (MQ=255)
                                                                                                                                                                                      tCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTg                                                           <  1:41259/251‑1 (MQ=255)
                                                                                                                                                                                            ccGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAAt                                                     >  1:312404/1‑251 (MQ=255)
                                                                                                                                                                                              gTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGTACAAGCGTCCACACTTGTGCTGAAAatatata                                                                                                                                                                                         >  1:549663/1‑118 (MQ=255)
                                                                                                                                                                                                   tGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAatat                                              <  1:865358/251‑1 (MQ=255)
                                                                                                                                                                                                     agaTCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCcac                                                                                                                                                                                                             >  1:773997/1‑91 (MQ=255)
                                                                                                                                                                                                      gaTCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGGCCACACTTGAGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAatatat                                                                                                  >  1:33476/1‑196 (MQ=255)
                                                                                                                                                                                                        tCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGt                                         >  1:225448/1‑251 (MQ=255)
                                                                                                                                                                                                        tCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGt                                         <  1:761663/251‑1 (MQ=255)
                                                                                                                                                                                                                       aTGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAAc                                                                                       >  1:315774/1‑190 (MQ=255)
                                                                                                                                                                                                                        tGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTcagcag                                                                                                                                           >  1:145614/1‑138 (MQ=255)
                                                                                                                                                                                                                        tGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCAgggggggct                                                                                                                                   >  1:688360/1‑144 (MQ=255)
                                                                                                                                                                                                                        tGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAAttt                                                                          >  1:798919/1‑202 (MQ=255)
                                                                                                                                                                                                                        tGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAatat                                              <  1:35251/230‑1 (MQ=255)
                                                                                                                                                                                                                        tGGTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTa                          >  1:393869/1‑250 (MQ=255)
                                                                                                                                                                                                                          gTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAgcgc                                                                                                                                                                                                                         >  1:389779/1‑58 (MQ=255)
                                                                                                                                                                                                                          gTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAg                                                                                                                                                                 >  1:6856/1‑114 (MQ=255)
                                                                                                                                                                                                                          gTACGGCTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAAt                                                                                                                         >  1:294406/1‑153 (MQ=255)
                                                                                                                                                                                                                                cTGGGCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCGCACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGAc                  <  1:848668/250‑1 (MQ=255)
                                                                                                                                                                                                                                   ggCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAat                                                                                                      >  1:594811/1‑163 (MQ=255)
                                                                                                                                                                                                                                   ggCGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGt                                                          >  1:190765/1‑207 (MQ=255)
                                                                                                                                                                                                                                     cGTGGAAAGTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCAgggggggct                                                                                                                                   >  1:281734/1‑131 (MQ=255)
                                                                                                                                                                                                                                             gTGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGACTTGGAgg           <  1:573002/244‑1 (MQ=255)
                                                                                                                                                                                                                                              tGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGACTTGGAGGTTatatat   <  1:639762/251‑1 (MQ=255)
                                                                                                                                                                                                                                              tGCGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGACTTGGAGGTTatatat   <  1:399492/251‑1 (MQ=255)
                                                                                                                                                                                                                                                cGGTGGTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCA‑GGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGACTTGGAGGTTATATatg  <  1:682604/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
AATATTTATTGATAAGTTTCGACATCAGCCCGATTGCCCAAATCAAAATGAGCAAAAAGACCAACACAAAACCCATCCCTGAAAACATCAGATTAATTCCTTCACCGAAAAGTTCTGCCGAAGTCATCAGTCGTTTCCTCATATTTTTGAAATGGGAGTTTCATTCTAGCGGATTCACTCCATCAAAACCGTGATTGAGATCATAATTTACGCCGATGGTACGGCTGGGCGTGGAAAGTGCGGTGTTTTTTTACGATGAATTTTAAACGATAGCGCACAAGCGTCCACACTTGTGCTGAAAATATATATTTGTAGAAATAAAAAAGTACAAGTGAAAAACGCTTGCGTCAGCAGGGGGGGGCTATGGGAAATTTCCTAATTACATAAAAATATATTAATAAAAAACTGTTCTATAATTTTGACTGTACTTTTTGTTCAATGTAATATGATGTATCTCAACGGTTGTACTAAAGACTTGGAGGTTATATATG  >  1/1220723‑1221213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑