Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,215,148	G→T	intergenic (+55568/+205789)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,215,148	0	G	T	100.0%	36.3 / NA	15	intergenic (+55568/+205789)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/10);  total (5/10)

AAAACTGGGGGTTACAGACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCGGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAACAGCGCTTTGATATTCGCGATATGACATTACCTGCATATATC  >  1/1214903‑1215396                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         aaaaCTGGGGGTTACAGACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaac                                                                                                                                                                                                                                                     <  1:696615/251‑2 (MQ=255)   aaCTGGGGGTTACAGACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaacca                                                                                                                                                                                                                                                   <  1:532549/251‑4 (MQ=255)           gTTACAGACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGTTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaac                                                                                                                                                                                                                                                 <  1:237534/245‑6 (MQ=255)           gTTACAGACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaac                                                                                                                                                                                                                                                     <  1:356418/241‑2 (MQ=255)                 gACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatatta                                                                                                                                                                                                                                     >  1:817213/1‑234 (MQ=255)                 gACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatatta                                                                                                                                                                                                                                     >  1:44615/1‑234 (MQ=255)                 gACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatatta                                                                                                                                                                                                                                     >  1:729429/1‑234 (MQ=255)                 gACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaat                                                                                                                                                                                                                                         <  1:82989/247‑14 (MQ=255)                    tAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatattagaa                                                                                                                                                                                                                                  <  1:79751/251‑21 (MQ=255)                    tAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatattagaa                                                                                                                                                                                                                                  <  1:741437/251‑21 (MQ=255)                    tAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatattagaa                                                                                                                                                                                                                                  <  1:593489/251‑21 (MQ=255)                           gCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaac                                                                                                                                                                                                                                                     <  1:590650/225‑2 (MQ=255)                                                 cTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatatt                                                                                                                                                                                                                                      >  1:663501/1‑202 (MQ=255)                                                       cacaTTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaatattagaa                                                                                                                                                                                                                                  <  1:121354/216‑21 (MQ=255)                                                                                                        cATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCTGTaaccaaccacttaat                                                                                                                                                                                                                                         >  1:508716/1‑147 (MQ=255)                                                                                                                                                                                                                               atctcgcggtcggcaacacgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAACAGCGCTTTGATATTCGCGat                       >  1:596900/19‑251 (MQ=255)                                                                                                                                                                                                                                       gtcggcaacacgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATAc                                                <  1:820994/208‑1 (MQ=255)                                                                                                                                                                                                                                       gtcggcaacacgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAAc                                           >  1:84937/11‑223 (MQ=255)                                                                                                                                                                                                                                         cggcaacacgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAACAGCGCTTTGATATTCGCGATATGACATTa              >  1:305776/9‑250 (MQ=255)                                                                                                                                                                                                                                           gcaacacgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAAc                                           <  1:445417/213‑1 (MQ=255)                                                                                                                                                                                                                                                 cgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTcc                                                                                                                                                                                                      >  1:497607/1‑58 (MQ=255)                                                                                                                                                                                                                                                 cgtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGc                                                               >  1:557833/1‑193 (MQ=255)                                                                                                                                                                                                                                                  gtccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAACAGCGCTTTGATATTCGCGATATGACATTACCTGCatata    >  1:320720/1‑251 (MQ=255)                                                                                                                                                                                                                                                    ccgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAACAGCGCTTTGATATTCGCGATATGACATTACCTGCATATATc  <  1:539473/251‑1 (MQ=255)                                                                                                                                                                                                                                                     cgGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTc                                                                                                        >  1:369503/1‑148 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         AAAACTGGGGGTTACAGACTAAGAACGCCTATGCCAGTAAAAATCGTGCTTCCACACATTCCATTGCAATAGCGGGAAAAAACGATGGGTTTGAGGGTCTATTCATCTACACGGATCGTCGAGGCAAAGAAGCACAAATTCATCGTGATGCACGCAGGGGAAGACAGCGTTATTCCCGCCAGGTTGCCGTACCGGGTACGGGCGGTACATATAACTATTTTCTTTTGCAAGAGGATTGTCCGTCCGGTAATGTTGATTTATGCAAAGGTTACATAAAGCCACTGGCAAGAGTTATTCCTGAGCAAGAAGAGACGGCTAGTCAGGGAGAGTATACCGGTGAGAATCGTGTTGCGCCGAACCCGATGAAATCTCGTAGTCAATCTTGGTTTTTCCGCCCGGGTTATCACTTTTCCGAGCAACATTATCTCGGGGCGGTTATGGAATATACTAAACAGCGCTTTGATATTCGCGATATGACATTACCTGCATATATC  >  1/1214903‑1215396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑