Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,212,069	G→A	intergenic (+52489/+208868)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,212,069	0	G	A	100.0%	206.0 / NA	76	intergenic (+52489/+208868)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/38);  total (38/38)

GTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGGTTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGACCGTCTTCCGTTTGCATCAACGGTG  >  1/1211824‑1212314                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTc                                                                                                                                                                                                                                                    >  1:60842/1‑249 (MQ=255) gTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTc                                                                                                                                                                                                                                                    >  1:480815/1‑249 (MQ=255)  ttCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTcc                                                                                                                                                                                                                                                   <  1:692324/249‑1 (MQ=255)    ccAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGAc                                                                                                                                                                                                                                               <  1:350181/251‑1 (MQ=255)                  tCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAg                                                                                                                                                                                                                                 >  1:218074/1‑251 (MQ=255)                   cGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAgg                                                                                                                                                                                                                                <  1:766609/251‑1 (MQ=255)                     ttATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGAc                                                                                                                                                                                                                                               <  1:770563/234‑1 (MQ=255)                     ttATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGc                                                                                                                                                                                                                               <  1:471166/250‑1 (MQ=255)                       aTCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCCTTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTg                                                                                                                                                                                                                            >  1:698716/1‑251 (MQ=255)                       aTCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCt                                                                                                                                                                                                                              >  1:678425/1‑249 (MQ=255)                       aTCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTg                                                                                                                                                                                                                            >  1:354283/1‑251 (MQ=255)                       aTCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTg                                                                                                                                                                                                                            >  1:551445/1‑251 (MQ=255)                       aTCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTAAAGGccgt                                                                                                                                                                                                                            >  1:269598/1‑248 (MQ=255)                         ccaccaGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTggg                                                                                                                                                                                                                          >  1:335894/1‑251 (MQ=255)                                ggTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAggg                                                                                                                                                                                                                    >  1:567646/1‑250 (MQ=255)                                ggTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAggg                                                                                                                                                                                                                    <  1:135314/250‑1 (MQ=255)                                 gTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCg                                                                                                                                                                                                                  >  1:746605/1‑251 (MQ=255)                                                        gTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGaat                                                                                                                                                                                           >  1:669818/1‑251 (MQ=255)                                                        gTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGaat                                                                                                                                                                                           >  1:105568/1‑251 (MQ=255)                                                        gTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAAGTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGaat                                                                                                                                                                                           >  1:304881/1‑251 (MQ=255)                                                                     tCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCgtgt                                                                                                                                                                              <  1:106420/251‑1 (MQ=255)                                                                       gCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGaataa                                                                                                                                                                                         <  1:210220/238‑1 (MQ=255)                                                                       gCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGaat                                                                                                                                                                                           >  1:716379/1‑236 (MQ=255)                                                                                cACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATg                                                                                                                                                                    >  1:267400/1‑250 (MQ=255)                                                                                        gAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGc                                                                                                                                                                                                                               <  1:349588/183‑1 (MQ=255)                                                                                        gAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGaa                                                                                                                                                             <  1:62997/249‑1 (MQ=255)                                                                                        gAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGaa                                                                                                                                                             <  1:629357/249‑1 (MQ=255)                                                                                        gAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAAcg                                                                                                                                                           >  1:551670/1‑251 (MQ=255)                                                                                             aaTGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTc                                                                                                                                                       <  1:206179/250‑1 (MQ=255)                                                                                                 cgcCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCGTGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAggg                                                                                                                                                  <  1:597772/251‑1 (MQ=255)                                                                                                   ccTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACg                                                                                                                                                                                                  <  1:107274/201‑1 (MQ=255)                                                                                                   ccTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGc                                                                                                                                                 <  1:333670/250‑1 (MQ=255)                                                                                                       cATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAAc                                                                                                                                                            >  1:713163/1‑235 (MQ=255)                                                                                                       cATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCAt                                                                                                                                            >  1:646718/1‑251 (MQ=255)                                                                                                       cATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCAt                                                                                                                                            >  1:438721/1‑251 (MQ=255)                                                                                                        aTTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCAtt                                                                                                                                           <  1:95348/251‑1 (MQ=255)                                                                                                                    tAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATg                                                                                                                                                                                       <  1:880352/195‑1 (MQ=255)                                                                                                                    tAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATa                                                                                                                                 >  1:721655/1‑249 (MQ=255)                                                                                                                    tAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATa                                                                                                                                 >  1:496289/1‑249 (MQ=255)                                                                                                                     aaTTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACtt                                                                                                                              <  1:644154/251‑1 (MQ=255)                                                                                                                        tGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTggg                                                                                                                           <  1:157412/251‑1 (MQ=255)                                                                                                                        tGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTggg                                                                                                                           <  1:400469/251‑1 (MQ=255)                                                                                                                         gggCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAggg                                                                                                                                                  >  1:438816/1‑227 (MQ=255)                                                                                                                          ggCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGt                                                                                                                          <  1:590443/250‑1 (MQ=255)                                                                                                                             aaaTGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTc                                                                                                                      <  1:166752/251‑1 (MQ=255)                                                                                                                              aaTGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTCAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTc                                                                                                                      >  1:84583/1‑250 (MQ=255)                                                                                                                                                  cATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCAc                                                                                                                 >  1:200673/1‑235 (MQ=255)                                                                                                                                                    tGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTggg                                                                                                                                                                                                              <  1:765699/140‑1 (MQ=255)                                                                                                                                                    tGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGcc                                                                                                                                                                                     >  1:387562/1‑165 (MQ=255)                                                                                                                                                      aTTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTACTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTggg                                                                                                                           <  1:19542/221‑1 (MQ=255)                                                                                                                                                       ttACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAgg                                                                                            <  1:807314/251‑1 (MQ=255)                                                                                                                                                            ttCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATa                                                                                              >  1:185203/1‑244 (MQ=255)                                                                                                                                                                               tttATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGa                                                                    <  1:174549/251‑1 (MQ=255)                                                                                                                                                                                         gTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACgg                                                                          <  1:51368/235‑1 (MQ=255)                                                                                                                                                                                          tGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGccc                                                         <  1:563980/251‑1 (MQ=255)                                                                                                                                                                                           gAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGa                                                                                                                                                                                                             >  1:688384/1‑102 (MQ=255)                                                                                                                                                                                           gAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGaa                                                                                                                                                             >  1:698879/1‑150 (MQ=255)                                                                                                                                                                                           gAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGc                                                                                                        <  1:192552/203‑1 (MQ=255)                                                                                                                                                                                           gAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCccg                                                        >  1:498587/1‑251 (MQ=255)                                                                                                                                                                                            aaaaTATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCccg                                                        >  1:437959/1‑250 (MQ=255)                                                                                                                                                                                                 atCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGaa                                                                                                                                                             <  1:716726/144‑1 (MQ=255)                                                                                                                                                                                                 atCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAggg                                                                                                                                                  >  1:107533/1‑155 (MQ=255)                                                                                                                                                                                                 atCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTggg                                                                                                                           <  1:630773/178‑1 (MQ=255)                                                                                                                                                                                                 atCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGaaa                                                  >  1:358647/1‑251 (MQ=255)                                                                                                                                                                                                            cgcgACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGAt                                       <  1:542406/251‑1 (MQ=255)                                                                                                                                                                                                                 ccGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGAttctt                                   >  1:877067/1‑250 (MQ=255)                                                                                                                                                                                                                  cGGACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTcc                                 <  1:662946/251‑1 (MQ=255)                                                                                                                                                                                                                    gACAAATTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCa                                >  1:355731/1‑250 (MQ=255)                                                                                                                                                                                                                         aTTCACCACCAAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGAc                          <  1:747747/251‑1 (MQ=255)                                                                                                                                                                                                                              ccaccaAAATTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAggg                                                                                                                                                  >  1:11500/1‑126 (MQ=255)                                                                                                                                                                                                                                   aaaaTTTGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGACCGTCTTCCGt                <  1:74882/251‑1 (MQ=255)                                                                                                                                                                                                                                        ttGCGCTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGACCGTCTTCCGTTTGCa           <  1:709582/251‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGc                                                                                 <  1:90758/176‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGACCGTCTTCCGTTTGCATCaa       >  1:672376/1‑250 (MQ=255)                                                                                                                                                                                                                                               ttATTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAAt                                                                                               <  1:807197/160‑1 (MQ=255)                                                                                                                                                                                                                                                 aTTTGATTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGACCGCCTTCCGTTTGCATCAACGGTg  >  1:250467/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACATTCCTTTTTCATTTAAAATTTTATCTACGGTGAAAATATCTTTATCGCCGCGACCGGACAAATTCACCACCAAAATTTGCGCTTTATTTGGTTCCTGACGGATCATTTTTAAGGCTTGGGCGAGGGCGTGGGAGCTTTCTAACGCAGGAATAATGCCTTCGTGTTTTGCCAATGCTTGGAACGCGTCAAGGGCTTCATTATCGGTAATACTTGGGTATTCCGCACGACCGATGCTTTGTAAATAGGCGTGTTGCGGCCCCACGGAAGGGAAATCCAAGCCCGCCGAAATGGAATAGGATTCTTCCACTTGACCGTCTTCCGTTTGCATCAACGGTG  >  1/1211824‑1212314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑