Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,211,734	T→C	intergenic (+52154/+209203)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,211,734	0	T	C	100.0%	177.1 / NA	64	intergenic (+52154/+209203)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/32);  total (32/32)

CGTCTGCATTCGGCGGGCAAATAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTTTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACAT  >  1/1211494‑1211979                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      cGTCTGCATTCGGCGGGCAAATAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCtg                                                                                                                                                                                                                                             <  1:745924/251‑1 (MQ=255) cGTCTGCATTCGGCGGGCAAATAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCtg                                                                                                                                                                                                                                             <  1:211154/251‑1 (MQ=255) cGTCTGCATTCGGCGGGCAAATAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCtg                                                                                                                                                                                                                                             <  1:499827/251‑1 (MQ=255)     tGCATTCGGCGGGCAAATAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGAc                                                                                                                                                                                                                                         <  1:404770/251‑1 (MQ=255)                    aaTAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCa                                                                                                                                                                                                                           >  1:90326/1‑250 (MQ=255)                    aaTAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCa                                                                                                                                                                                                                           >  1:607691/1‑250 (MQ=255)                    aaTAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCa                                                                                                                                                                                                                           >  1:576339/1‑250 (MQ=255)                      tAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGa                                                                                                                                                                                                                        >  1:410124/1‑251 (MQ=255)                              ggTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTgg                                                                                                                                                                                                                 >  1:532612/1‑250 (MQ=255)                               gTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTggc                                                                                                                                                                                                                <  1:702787/250‑1 (MQ=255)                                                          cgACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATaa                                                                                                                                                                                     <  1:867548/250‑1 (MQ=255)                                                          cgACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATaa                                                                                                                                                                                     <  1:431646/250‑1 (MQ=255)                                                            aCATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATaa                                                                                                                                                                                     >  1:39785/1‑248 (MQ=255)                                                            aCATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATaa                                                                                                                                                                                     >  1:229336/1‑248 (MQ=255)                                                            aCATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAggg                                                                                                                                                                                  <  1:586614/251‑1 (MQ=255)                                                            aCATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAggg                                                                                                                                                                                  <  1:890403/251‑1 (MQ=255)                                                                   cGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGgag                                                                                                                                                                           <  1:707696/251‑1 (MQ=255)                                                                     tCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAAc                                                                                                                                                                                                                              <  1:511461/198‑1 (MQ=255)                                                                     tCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGaa                                                                                                                                                                         >  1:145357/1‑251 (MQ=255)                                                                               acaacGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAAccc                                                                                                                                                               <  1:655125/251‑1 (MQ=255)                                                                                   cGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGgag                                                                                                                                                                           >  1:878274/1‑235 (MQ=255)                                                                                     gAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTc                                                                                                                                                          >  1:357752/1‑250 (MQ=255)                                                                                               aaccaacACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCg                                                                                                                                               >  1:614076/1‑251 (MQ=255)                                                                                                accaacACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCg                                                                                                                                               >  1:74694/1‑250 (MQ=255)                                                                                                 ccaacACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGc                                                                                                                                              >  1:431645/1‑250 (MQ=255)                                                                                                           gTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATcca                                                                                                                                   >  1:667008/1‑251 (MQ=255)                                                                                                           gTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATcca                                                                                                                                   >  1:745355/1‑251 (MQ=255)                                                                                                             ccACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATccacc                                                                                                                                 <  1:180522/251‑1 (MQ=255)                                                                                                               aCGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTTGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAg                                                                                                                               <  1:339263/251‑1 (MQ=255)                                                                                                                         cGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAaata                                                                                                                     <  1:369316/251‑1 (MQ=255)                                                                                                                                     gATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGAt                                                                                                                                                                                                                       <  1:682339/141‑1 (MQ=255)                                                                                                                                     gATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATaa                                                                                                                                                                                     >  1:799842/1‑175 (MQ=255)                                                                                                                                     gATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAg                                                                                                                               <  1:388115/229‑1 (MQ=255)                                                                                                                                      aTAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACTCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGaa                                                                                                         >  1:795394/1‑250 (MQ=255)                                                                                                                                      aTAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAAc                                                                                                        <  1:303794/251‑1 (MQ=255)                                                                                                                                          aaaTTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGc                                                                                                                          <  1:789221/229‑1 (MQ=255)                                                                                                                                          aaaTTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGt                                                                                                     >  1:475778/1‑250 (MQ=255)                                                                                                                                               aTCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGAt                                                                                                                                                                                <  1:586420/170‑1 (MQ=255)                                                                                                                                               aTCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCaaa                                                                                                                       <  1:266237/227‑1 (MQ=255)                                                                                                                                                acccaggcTTTGGCCAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGt                                                                                              <  1:578202/244‑1 (MQ=255)                                                                                                                                                              aaaaaTTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAgg                                                                                <  1:405125/251‑1 (MQ=255)                                                                                                                                                                aaattaaattGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAaataa                                                                                                                    >  1:35459/1‑213 (MQ=255)                                                                                                                                                                     aaattGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAggg                                                                                                                                                                                  <  1:677562/146‑1 (MQ=255)                                                                                                                                                                          ggCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGaa                                                                    >  1:386091/1‑251 (MQ=255)                                                                                                                                                                               aaaGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACAAAAGGAACGAAt                                                               <  1:590060/251‑1 (MQ=255)                                                                                                                                                                                aaGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATg                                                              >  1:143515/1‑251 (MQ=255)                                                                                                                                                                                  gcagcaAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATccacc                                                                                                                                 <  1:154552/182‑1 (MQ=255)                                                                                                                                                                                  gcagcaAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAg                                                                                                                               >  1:814158/1‑184 (MQ=255)                                                                                                                                                                                  gcagcaAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATgcg                                                            >  1:201051/1‑251 (MQ=255)                                                                                                                                                                                            cATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGAc                                                                                                                                                                                                                                         >  1:613016/1‑68 (MQ=255)                                                                                                                                                                                                   aTCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCgtg                                           >  1:222371/1‑251 (MQ=255)                                                                                                                                                                                                     ctcCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTa                                         >  1:242282/1‑251 (MQ=255)                                                                                                                                                                                                          gATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTgg                                    >  1:669142/1‑251 (MQ=255)                                                                                                                                                                                                            tatTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTggc                                                                                                                                                                                                                <  1:171255/77‑1 (MQ=255)                                                                                                                                                                                                            tatTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAAccc                                                                                                                                                               >  1:91827/1‑126 (MQ=255)                                                                                                                                                                                                            tatTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCAttt                                                   >  1:173278/1‑234 (MQ=255)                                                                                                                                                                                                                           ttttCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAaat                                                                                                                      <  1:258885/152‑1 (MQ=255)                                                                                                                                                                                                                            tttCCAGCAATTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCaaaa                  <  1:754655/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGt                                                                               >  1:49107/1‑181 (MQ=255)                                                                                                                                                                                                                                     aTTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTgcg                                               <  1:184469/213‑1 (MQ=255)                                                                                                                                                                                                                                     aTTTAAAGCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATg         >  1:728550/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttAAAGCTGTCTTCCGTGCTGTGACCGGAGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGAt       <  1:545452/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGgag                                                                                                                                                                           >  1:267996/1‑82 (MQ=255)                                                                                                                                                                                                                                             cTGTCTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACAt  <  1:90390/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CGTCTGCATTCGGCGGGCAAATAAAAACGGGTTGAATACCGTGTCTTTTCGCGGCGGCGACATAATCGTCTTTCGCCAACAACGGAATATCGGCAACCAACACGGCGTCCACGCCCACGTCGGCGCAACGCTGATAAAAATTATCCAAGCCTTTGGCAAAAATTAAATTGGCGCAAAGCAGCAAACTCATCGGAATCTCCGGATATTTTGACCGCACTTTTTCCAGCAATTTAAAGCTGTTTTCCGTGCTGTGACCGGCGTTTAACGCACGATTGTTGGCGGCTTGAATCACCGGTCCGTCCAATAAGGGATCGGAGAACGGAAAACCCAGTTCCAATGCGTCCGCCTCGTTATCCACCAGGGTGCAAATAATGTCAAAAGAACGGTCGAAAGTCGGGTCGCATAAGGTCACGAAAGGAACGAATGCGCCTTCATTTTGCGCGTGTAATTGGGCAAATGTGGTTTCAAAACGGCTCATGATTACAT  >  1/1211494‑1211979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑