Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,205,483	A→G	intergenic (+45903/+215454)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,205,483	0	A	G	100.0%	217.6 / NA	78	intergenic (+45903/+215454)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/43);  total (35/43)

TATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCCCCCGACAATCAGGAGGACGCGGAAGAATATTCAAAAGTGCTGGGCAATCGTACTGTAGATAAATTAAGCCATAGTCGCGGAAAAACGTCCAGTCACAGTATTACCGACCATGGGCGACCGTTGCTTTTACCACAGGAATTTAGACTAATCGGAGAATTTAAAGAAGTGGTGATGTTAAACAACAGTAAACCGATACTGTGTAATAAGGCGGTGTACTACAACGATCCTTATGCCGACCA  >  1/1205240‑1205584                                                                                                                                                                                                                                                    |                                                                                                      tatttatCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGat                                                                                                <  1:185774/251‑2 (MQ=255)      atcaatcaCAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATc                                                                                           <  1:881551/251‑1 (MQ=255)            cacaAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATc                                                                                           >  1:492632/1‑245 (MQ=255)                 gggCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTaa                                                                                >  1:40228/1‑251 (MQ=255)                              ccGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATg                                                                   <  1:264383/251‑1 (MQ=255)                                gATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGtt                                                                 >  1:412564/1‑251 (MQ=255)                                 aTAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGtt                                                                 <  1:415687/250‑1 (MQ=255)                                     gCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            >  1:381475/1‑249 (MQ=255)                                     gCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            >  1:32122/1‑249 (MQ=255)                                     gCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            >  1:752253/1‑249 (MQ=255)                                     gCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            >  1:625294/1‑249 (MQ=255)                                     gCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            >  1:688951/1‑249 (MQ=255)                                     gCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            >  1:186147/1‑249 (MQ=255)                                       cTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAaataac                                                          <  1:578807/251‑1 (MQ=255)                                       cTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAaataac                                                          <  1:309491/251‑1 (MQ=255)                                       cTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAaataac                                                          <  1:341106/251‑1 (MQ=255)                                                 cGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGa                                                >  1:314932/1‑251 (MQ=255)                                                            gTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTCCTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaat                                                             >  1:434323/1‑226 (MQ=255)                                                             tGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAGCCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTTCTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatgtaat                                     <  1:424435/250‑11 (MQ=255)                                                             tGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCATAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatgtaat                                     <  1:486228/250‑11 (MQ=255)                                                             tGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatgtaat                                     <  1:371289/250‑11 (MQ=255)                                                             tGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatgtaat                                     <  1:309138/250‑11 (MQ=255)                                                                 aaaGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGTTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:451822/251‑1 (MQ=255)                                                                 aaaGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:569444/251‑1 (MQ=255)                                                                 aaaGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:48082/251‑1 (MQ=255)                                                                 aaaGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:286263/251‑1 (MQ=255)                                                                 aaaGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:575570/251‑1 (MQ=255)                                                                   aGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATc                                                                                           >  1:178008/1‑190 (MQ=255)                                                                       aTCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATg                                                                   >  1:91508/1‑210 (MQ=255)                                                                       aTCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:894013/245‑1 (MQ=255)                                                                        tCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAaag                                                                              <  1:826163/198‑1 (MQ=255)                                                                           tGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGtattac                      <  1:371636/251‑1 (MQ=255)                                                                            ggATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACa                     >  1:155298/1‑251 (MQ=255)                                                                            ggATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACa                     >  1:369443/1‑251 (MQ=255)                                                                            ggATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACa                     >  1:197858/1‑251 (MQ=255)                                                                            ggATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACa                     >  1:624122/1‑251 (MQ=255)                                                                              ataataTCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:103576/250‑1 (MQ=255)                                                                              ataataTCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:749766/250‑1 (MQ=255)                                                                              ataataTCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:718894/250‑1 (MQ=255)                                                                              ataataTCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:37047/250‑1 (MQ=255)                                                                              ataataTCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAAc                   <  1:530609/251‑1 (MQ=255)                                                                              ataataTCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAAc                   <  1:848076/251‑1 (MQ=255)                                                                                 atatCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaat                                                             >  1:568855/1‑205 (MQ=255)                                                                                   atCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATcc              <  1:769815/251‑1 (MQ=255)                                                                                     cGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAgc                                                       <  1:254296/208‑2 (MQ=255)                                                                                          gTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAAc                                                   >  1:7716/1‑207 (MQ=255)                                                                                               aTTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTAttttataga  >  1:420535/1‑243 (MQ=255)                                                                                               aTTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGCAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGtattac                      >  1:287737/1‑231 (MQ=255)                                                                                                  cTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaata                                                            <  1:327104/190‑3 (MQ=255)                                                                                                  cTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:858786/230‑1 (MQ=255)                                                                                                    atatGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGat                                                                                                >  1:677598/1‑151 (MQ=255)                                                                                                    atatGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatgt                                        <  1:894996/208‑8 (MQ=255)                                                                                                                 aaccAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:684656/212‑1 (MQ=255)                                                                                                                   ccAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGtattac                      >  1:79394/2‑211 (MQ=255)                                                                                                                              cagaagaaTATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    >  1:464836/3‑202 (MQ=255)                                                                                                                                gaagaaTATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaat                                                             >  1:36872/1‑158 (MQ=255)                                                                                                                                     atatTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGat                                                                                                >  1:228695/1‑118 (MQ=255)                                                                                                                                     atatTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCACAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                >  1:514593/1‑183 (MQ=255)                                                                                                                                       atTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatg                                         <  1:500270/172‑7 (MQ=255)                                                                                                                                       atTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTAttttat     >  1:659792/1‑203 (MQ=255)                                                                                                                                            aaaGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatg                                         >  1:595757/1‑161 (MQ=255)                                                                                                                                               gTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTaaat                                                             >  1:805268/1‑143 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAgtgg                                                                        <  1:205329/111‑1 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAgtgg                                                                        <  1:825948/111‑1 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                >  1:308318/5‑155 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:491100/151‑1 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGtattac                      <  1:795909/161‑1 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    >  1:790102/5‑167 (MQ=255)                                                                                                                                                                 gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTAttttatag   >  1:200735/5‑177 (MQ=255)                                                                                                                                                                   aaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatgt                                        >  1:378397/3‑138 (MQ=255)                                                                                                                                                                   aaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAtta                                             <  1:23303/138‑3 (MQ=255)                                                                                                                                                                            aaGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGc                                <  1:734341/144‑1 (MQ=255)                                                                                                                                                                                 aTAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    >  1:185440/1‑151 (MQ=255)                                                                                                                                                                                        aggtaaaaCATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATg                                                                   <  1:796441/93‑1 (MQ=255)                                                                                                                                                                                        aggtaaaaCATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGtaataa                                   <  1:806651/125‑1 (MQ=255)                                                                                                                                                                                        aggtaaaaCATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTAtttta      <  1:114465/154‑5 (MQ=255)                                                                                                                                                                                                            gTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACaa                    <  1:760081/124‑1 (MQ=255)                                                                                                                                                                                                                                               caGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGAttatatg                                         <  1:395785/68‑7 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                      TATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCCCCCGACAATCAGGAGGACGCGGAAGAATATTCAAAAGTGCTGGGCAATCGTACTGTAGATAAATTAAGCCATAGTCGCGGAAAAACGTCCAGTCACAGTATTACCGACCATGGGCGACCGTTGCTTTTACCACAGGAATTTAGACTAATCGGAGAATTTAAAGAAGTGGTGATGTTAAACAACAGTAAACCGATACTGTGTAATAAGGCGGTGTACTACAACGATCCTTATGCCGACCA  >  1/1205240‑1205584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑