Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,204,994	C→T	intergenic (+45414/+215943)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,204,994	0	C	T	100.0%	201.1 / NA	74	intergenic (+45414/+215943)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/40);  total (34/40)

CAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACACGCTACCTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGT  >  1/1204764‑1205219                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  cAAGAAGACGGTTCGATAGAACATATCGTTTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAACAGTATCGATTCAACCTTTATCAGTCCGCTAACCATTTTTCAAAATGAAATTGTTGAATTTGCGACTTCTGCCCGTGATTTTGTTTTACGCGCTTTACGCAAGGAAAAAATcc                                                                                                                                                                                                                >  1:608590/1‑249 (MQ=255) cAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCa                                                                                                                                                                                                                >  1:374650/1‑250 (MQ=255)                aTAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACAt                                                                                                                                                                                                <  1:792956/251‑1 (MQ=255)                    aaCATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCg                                                                                                                                                                                            >  1:593985/1‑251 (MQ=255)                                    acaacaTGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATcc                                                                                                                                                                            <  1:62452/251‑1 (MQ=255)                                        caTGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGGGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTgg                                                                                                                                                                         >  1:703888/1‑250 (MQ=255)                                         aTGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATc                                                                                                                                                                             >  1:258321/1‑245 (MQ=255)                                                cTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATc                                                                                                                                                                             >  1:767467/1‑238 (MQ=255)                                                  tATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGt                                                                                                                                                               >  1:617929/1‑250 (MQ=255)                                                              ccGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGttt                                                                                                                                                  <  1:221261/251‑1 (MQ=255)                                                                 gCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:9428/243‑1 (MQ=255)                                                                   aTTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAg                                                                                                                                             <  1:211715/251‑1 (MQ=255)                                                                    ttGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATcc                                                                                                                                                                            <  1:594534/219‑1 (MQ=255)                                                                      gCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTTTTGAATATGCGACTTCTGCCAGGGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCAt                                                                                                                                          >  1:898984/1‑251 (MQ=255)                                                                      gCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCAt                                                                                                                                          >  1:556728/1‑251 (MQ=255)                                                                      gCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCAt                                                                                                                                          >  1:516079/1‑251 (MQ=255)                                                                        tCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGAAAAAAATCACGATTTATTTc                                                                                                                                                                                                     >  1:439153/1‑190 (MQ=255)                                                                         cACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAg                                                                                                                                             >  1:891959/1‑245 (MQ=255)                                                                         cACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGt                                                                                                                                       <  1:778923/251‑1 (MQ=255)                                                                           ccAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTa                                                                                                                                     >  1:881987/1‑251 (MQ=255)                                                                              gACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCaa                                                                                                                                                                 <  1:61321/220‑1 (MQ=255)                                                                              gACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTg                                                                                                                                  >  1:581931/1‑251 (MQ=255)                                                                                ctactGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:820847/251‑1 (MQ=255)                                                                                ctactGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:275739/251‑1 (MQ=255)                                                                                  actGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAg                                                                                                                                             <  1:376767/236‑1 (MQ=255)                                                                                     gCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCcaacaa                                                                                                                           >  1:107861/1‑251 (MQ=255)                                                                                      ccGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAat                                                                                                                          >  1:186430/1‑251 (MQ=255)                                                                                         aTAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAatat                                                                                                                        <  1:332869/250‑1 (MQ=255)                                                                                                cAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:761522/235‑1 (MQ=255)                                                                                                      ttatttaATGGCGTGTGAATCCGGTAAGACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCgg                                                                                                          >  1:100805/1‑251 (MQ=255)                                                                                                      ttatttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:145161/206‑1 (MQ=255)                                                                                                        atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGcc                                                                                                                                <  1:66185/227‑1 (MQ=255)                                                                                                        atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGa                                                                                                                     >  1:747595/1‑238 (MQ=255)                                                                                                        atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGaa                                                                                                        >  1:413599/1‑251 (MQ=255)                                                                                                           taATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAaacc                                                                                                     <  1:267617/251‑1 (MQ=255)                                                                                                                    gtgAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACa                                                                                            >  1:249560/1‑251 (MQ=255)                                                                                                                          ccGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTaaaa                                                                                      <  1:397684/251‑1 (MQ=255)                                                                                                                          ccGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTaaaa                                                                                      <  1:2814/251‑1 (MQ=255)                                                                                                                           cGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATcc                                                                                                                                                                            >  1:411356/1‑164 (MQ=255)                                                                                                                              tAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAatat                                                                                   <  1:517120/250‑1 (MQ=255)                                                                                                                                  aCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtt                                                                              <  1:453448/251‑1 (MQ=255)                                                                                                                                  aCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtt                                                                              >  1:459126/1‑251 (MQ=255)                                                                                                                                       aaGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAAt                                                                                                                                                                                                                  <  1:549789/114‑1 (MQ=255)                                                                                                                                       aaGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGc                                                                                                                                                                        <  1:832096/156‑1 (MQ=255)                                                                                                                                            gTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATg                                                                                                                                               >  1:700238/1‑176 (MQ=255)                                                                                                                                             tATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtactac                                                                      <  1:542122/248‑1 (MQ=255)                                                                                                                                               tCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                 <  1:238581/251‑1 (MQ=255)                                                                                                                                                cGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATgg                                                                >  1:596482/1‑251 (MQ=255)                                                                                                                                                 gATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATc                                                                                                                                                                             <  1:298752/141‑1 (MQ=255)                                                                                                                                                 gATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:74762/163‑1 (MQ=255)                                                                                                                                                  aTTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGc                                                                                                                                                                                          >  1:856483/1‑127 (MQ=255)                                                                                                                                                      aaCCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtact                                                                        <  1:252071/237‑1 (MQ=255)                                                                                                                                                       aCCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATc                                                                                                                                                       <  1:601834/157‑1 (MQ=255)                                                                                                                                                       aCCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATg                                                                                                                                               <  1:564016/165‑1 (MQ=255)                                                                                                                                                       aCCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAatat                                                                                                                        >  1:708687/1‑188 (MQ=255)                                                                                                                                                       aCCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGtt                                                         >  1:195089/1‑251 (MQ=255)                                                                                                                                                       aCCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGt                                                          >  1:48118/1‑250 (MQ=255)                                                                                                                                                         cTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACAt                                                                                           >  1:164068/1‑215 (MQ=255)                                                                                                                                                           ttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAat                                                                                     <  1:386040/219‑1 (MQ=255)                                                                                                                                                                   ccGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATg                                                             <  1:465579/235‑1 (MQ=255)                                                                                                                                                                        aaCCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGt                                        <  1:570178/251‑1 (MQ=255)                                                                                                                                                                          ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                      <  1:518562/251‑1 (MQ=255)                                                                                                                                                                          ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                      >  1:285079/1‑251 (MQ=255)                                                                                                                                                                             tttttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGc                                   <  1:509584/251‑1 (MQ=255)                                                                                                                                                                               tttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                 <  1:632859/251‑1 (MQ=255)                                                                                                                                                                                  cAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTAt                              <  1:608420/251‑1 (MQ=255)                                                                                                                                                                                       tGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATa                         >  1:786649/1‑251 (MQ=255)                                                                                                                                                                                              gttgaatatgcgacttctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                 <  1:598106/221‑1 (MQ=255)                                                                                                                                                                                                  aatatgcgacttctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAgg               <  1:135605/239‑1 (MQ=255)                                                                                                                                                                                                   atatgcgacttctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtact                                                                        <  1:63564/182‑1 (MQ=255)                                                                                                                                                                                                   atatgcgacttctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                 >  1:240801/11‑199 (MQ=255)                                                                                                                                                                                                          acttctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGc      <  1:48175/248‑1 (MQ=255)                                                                                                                                                                                                             tctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCgg   >  1:844166/1‑251 (MQ=255)                                                                                                                                                                                                              ctGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGt  >  1:752925/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  CAAGAAGACGGTTCGATAGAACATATCGATTTTGCACAACATGCGCGCTTATGCGTTCAATCCGGCATTGCTCACCAGACTACTGCCGATAAACTCAATATTTATTTAATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACACGCTACCTCTGCCAGTGATTTTGATTTACGCGCCTTGCGCCGGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGT  >  1/1204764‑1205219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑