Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	256,265	C→T	intergenic (+122954/+5966)	unknown → / ← unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	256,265	0	C	T	100.0%	223.2 / NA	77	intergenic (+122954/+5966)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/42);  total (35/42)

GTTCAATCCAACCGGATTGTTTGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGCTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGCCATATTCGTCATCATCGGCATC  >  1/256018‑256488                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                gTTCAATCCAACCGGATTGTTTGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTtcg                                                                                                                                                                                                                              >  1:231064/1‑251 (MQ=255) gTTCAATCCAACCGGATTGTTTGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACAAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTtcg                                                                                                                                                                                                                              >  1:425531/1‑251 (MQ=255)          aaCCGGATTGTTTGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAAcc                                                                                                                                                                                                                     <  1:629767/251‑1 (MQ=255)          aaCCGGATTGTTTGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAAcc                                                                                                                                                                                                                     <  1:628694/251‑1 (MQ=255)                      tGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCg                                                                                                                                                                                                         <  1:542730/251‑1 (MQ=255)                                 tCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCgg                                                                                                                                                                                                                          >  1:580942/1‑223 (MQ=255)                                   aTAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAg                                                                                                                                                                                            <  1:545352/251‑1 (MQ=255)                                    tAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAg                                                                                                                                                                                            >  1:763620/1‑250 (MQ=255)                                    tAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGc                                                                                                                                                                                           <  1:614863/251‑1 (MQ=255)                                        gCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACg                                                                                                                                                                                       >  1:273019/1‑251 (MQ=255)                                           cTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCtt                                                                                                                                                                                    >  1:843931/1‑251 (MQ=255)                                           cTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCtt                                                                                                                                                                                    >  1:90516/1‑251 (MQ=255)                                           cTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTAACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCtt                                                                                                                                                                                    >  1:451001/1‑251 (MQ=255)                                             tgtgtgTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTaa                                                                                                                                                                                  >  1:807141/1‑251 (MQ=255)                                             tgtgtgTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTaa                                                                                                                                                                                  >  1:623352/1‑251 (MQ=255)                                               tgtgtTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAg                                                                                                                                                                                 <  1:493559/250‑1 (MQ=255)                                                gtgtTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAggg                                                                                                                                                                               >  1:522407/1‑251 (MQ=255)                                                 tgtTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgc                                                                                                                                                                              <  1:521807/251‑1 (MQ=255)                                                 tgtTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgc                                                                                                                                                                              <  1:618288/251‑1 (MQ=255)                                                        cgTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgcaatagc                                                                                                                                                                        <  1:110076/250‑1 (MQ=255)                                                          tATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAggg                                                                                                                                                                               >  1:662404/1‑241 (MQ=255)                                                            tttttCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATaa                                                                                                                                                                   <  1:462039/251‑1 (MQ=255)                                                               ttCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAAc                                                                                                                                                                <  1:794895/251‑1 (MQ=255)                                                               ttCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAAc                                                                                                                                                                <  1:713005/251‑1 (MQ=255)                                                                     aTTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATc                                                                                                                                                          <  1:686850/251‑1 (MQ=255)                                                                            ccGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAAt                                                                                                                                                   <  1:332694/251‑1 (MQ=255)                                                                            ccGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAAt                                                                                                                                                   <  1:286467/251‑1 (MQ=255)                                                                              gCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAAttt                                                                                                                                                 >  1:511731/1‑251 (MQ=255)                                                                                 cTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgc                                                                                                                                                                              <  1:1275/219‑1 (MQ=255)                                                                                 cTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCAt                                                                                                                                              >  1:104699/1‑251 (MQ=255)                                                                                           tAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTcc                                                                                                                                    <  1:189591/251‑1 (MQ=255)                                                                                                 caTCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAAc                                                                                                                                                                >  1:760255/1‑217 (MQ=255)                                                                                                 caTCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTtc                                                                                                                                       >  1:278446/1‑242 (MQ=255)                                                                                                  aTCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAAc                                                                                                                                                                >  1:519860/1‑216 (MQ=255)                                                                                                  aTCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATaaaa                                                                                                                             >  1:291455/1‑251 (MQ=255)                                                                                                     cAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgc                                                                                                                                                                              >  1:122813/1‑199 (MQ=255)                                                                                                       aTGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCt                                                                                                                                                                                                               <  1:168429/164‑1 (MQ=255)                                                                                                       aTGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTaa                                                                                                                        >  1:251893/1‑251 (MQ=255)                                                                                                          cagcaTTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTaaaa                                                                                                                      <  1:79290/250‑1 (MQ=255)                                                                                                            gcaTTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTcc                                                                                                                                    <  1:398519/234‑1 (MQ=255)                                                                                                            gcaTTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGt                                                                                                                   >  1:148579/1‑251 (MQ=255)                                                                                                            gcaTTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGt                                                                                                                   >  1:224607/1‑251 (MQ=255)                                                                                                                   cTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGgttg                                                                                                            >  1:686590/1‑251 (MQ=255)                                                                                                                      tctcGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGttt                                                                                                         <  1:293971/251‑1 (MQ=255)                                                                                                                       ctcGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGtttt                                                                                                        >  1:321386/1‑251 (MQ=255)                                                                                                                            tAATCGCTTGGTTTAATTTTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACa                                                                                                    <  1:567938/250‑1 (MQ=255)                                                                                                                                 gCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCt                                                                                                                                                                                                               <  1:272576/138‑1 (MQ=255)                                                                                                                                 gCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAg                                                                                                                    >  1:165208/1‑229 (MQ=255)                                                                                                                                      gTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTcc                                                                                                                                    >  1:547040/1‑208 (MQ=255)                                                                                                                                                           aTTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGtc                                                                    <  1:401179/251‑1 (MQ=255)                                                                                                                                                              ttCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGt                                                                                                                                           <  1:530006/177‑1 (MQ=255)                                                                                                                                                              ttCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGtcat                                                                  >  1:658107/1‑250 (MQ=255)                                                                                                                                                                      tCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAgg                                                         <  1:589109/251‑1 (MQ=255)                                                                                                                                                                      tCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAgg                                                         <  1:542172/251‑1 (MQ=255)                                                                                                                                                                       cACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCg                                                                                                                                                                                          <  1:833363/121‑1 (MQ=255)                                                                                                                                                                       cACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGc                                                        <  1:259490/251‑1 (MQ=255)                                                                                                                                                                       cACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGc                                                        <  1:309161/251‑1 (MQ=255)                                                                                                                                                                       cACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGc                                                        <  1:721770/251‑1 (MQ=255)                                                                                                                                                                             aaGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCatcatc                                                  <  1:896734/251‑1 (MQ=255)                                                                                                                                                                                agTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCt                                                 <  1:342890/249‑1 (MQ=255)                                                                                                                                                                                   gACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAggg                                                                                                                                                                               >  1:105155/1‑120 (MQ=255)                                                                                                                                                                                   gACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgc                                                                                                                                                                              <  1:96173/121‑1 (MQ=255)                                                                                                                                                                                   gACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATc                                                                                                                                                          >  1:729510/1‑141 (MQ=255)                                                                                                                                                                                     cTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCaa                                          >  1:752864/1‑251 (MQ=255)                                                                                                                                                                                        ccATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGgc                                                                                                                                                                              >  1:885754/1‑116 (MQ=255)                                                                                                                                                                                        ccATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCaaaaa                                       >  1:224833/1‑251 (MQ=255)                                                                                                                                                                                                tCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCt                                                             <  1:656553/221‑1 (MQ=255)                                                                                                                                                                                                tCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCg                               >  1:85369/1‑251 (MQ=255)                                                                                                                                                                                                     aTCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCAt                           >  1:678717/1‑250 (MQ=255)                                                                                                                                                                                                        cACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGt                              <  1:564012/244‑1 (MQ=255)                                                                                                                                                                                                        cACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGcc                       <  1:291542/251‑1 (MQ=255)                                                                                                                                                                                                              tttCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGCCATATTc                 <  1:788793/251‑1 (MQ=255)                                                                                                                                                                                                               ttCGCGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGCCATATTCg                <  1:324396/251‑1 (MQ=255)                                                                                                                                                                                                                 cGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTg                                                                                                                  <  1:45618/151‑1 (MQ=255)                                                                                                                                                                                                                           gtgtTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGCCATATTCg                <  1:489406/239‑1 (MQ=255)                                                                                                                                                                                                                             gtTAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTtttaacg                                                                                      >  1:328068/1‑167 (MQ=255)                                                                                                                                                                                                                              ttAAAAGAAATAACGTTTTTTTCCGGTTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGCCATATTCGTCATCATCGGCATc  <  1:219922/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                GTTCAATCCAACCGGATTGTTTGCCTTTGTTGTCATAACGCACTTGTGTGTTACGCGTATTTTTCTTAATTTGCACCGCACTTTGTTTTTTAATCACATCCAATGCAGCATTCACTTTCTCGTTAATCGCTTGGTTCAATTCTTTTAGATTTTTATTTTCCTGTTTCACGAAAAGAGTGACTTCCATTACGTCATAATCCACTTCTTTCGTGACTTCAGTGTTAAAAGAAATAACGTTTTTTTCCGGCTCGTCGGTAACCTTAGCTTCGGCGTTGGCAGTTAAAGCGACGCTTAAGGGCAATAGCAATAAAACTAAATCTTTGAATTTCATGGTTTTCTCCTATAAAAATTAAAAAGTGCGGTTGTTTTTACAAATGTTTTTTAACGTTTAACGAACTCATCGTCATCATCTAAGGCATCATCTTCGTCAAAAAATTCACCGTCATCGCCATATTCGTCATCATCGGCATC  >  1/256018‑256488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑