Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	18,266	T→G	intergenic (–/+112868)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	18,266	0	T	G	100.0%	14.2 / NA	7	intergenic (–/+112868)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/3);  total (4/3)

ATAAGGTATAAAAGGGGGTTAAACGAGTTTAACATAACCAAATATCCGCGCTCATTCCATGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTATTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGGAATATATAACTATAAAGAGGTATATTTACATTTTATGCTACAAAAAAGAATTATTTTTATTTGAATATTTTATCGAAGAAGAAATACCCTAATTACGGGCAATAAAAAACCGAACTCATCGAAATGAATTCGGCTTTCTGATTTGATGTTCTACTCTAAAAGTGCGGTTAATTTCTTACTCATAAATCTTGAAGATTTACTT  >  1/18093‑18508                                                                                                                                                                              |                                                                                                                                                                                                                                                   ataAGGTATAAAAGGGGGTTAAACGAGTTTAACATAACCAAATATCCGCGCTCATTCCATGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTAGTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGAAATATATAAc                                                                                                                                                                                                  <  1:273020/224‑1 (MQ=255)      gTATAAAAGGGGGTTAAACGAGTTTAACATAACCAAATATCCGCGCTCATTCCATGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTAGTATAAAAATATCATTAAAgg                                                                                                                                                                                                                                <  1:219940/189‑1 (MQ=255)                 ggTTAAACGAGTTTAACATAACCAAATATCCGCGCTCATTCCATGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTAGTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGAAATATATAACTATAAAGAGGTATATTTACATTTTATGCTACAAAAAAGAatta                                                                                                                                                       >  1:813777/1‑251 (MQ=255)                                      ccAAATATCCGCGCTCATTCCATGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTAGTATaaaaa                                                                                                                                                                                                                                            >  1:519461/1‑145 (MQ=255)                                                           aTGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTAGTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGAAATATATAACTATAAAGAGGTATATTTACATTTTATGCTACAAAAAAGAATTATTTTTATTTGAATATTTTATCGAAGAAGAAATACCCTAATTa                                                                                                             <  1:217793/251‑1 (MQ=255)                                                                                                                                                                      atttattAGTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGAAATATATAACTATAAAGAGGTATATTTACATTTTATGCTACAAAAAAGAATTATTTTTATTTGAATATTTTATCGAAGAAGAAATACCCTAATTACGGGCAATAAAAAACCGAACTCATCGAAATGAATTCGGCTTTCTGATTTGATGTTCTACTCTAAAAGTGCGGTTAATTTCTTACTCATAAATCTTGAAGATTTACtt  >  1:195623/1‑251 (MQ=255)                                                                                                                                                                      atttattAGTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGAAATATATAACTATAAAGAGGTATATTTACATTTTATGCTACAAAAAAGAATTATTTTTATTTGAATATTTTATCGAAGAAGAAATACCCTAATTACGGGCAATAAAAAACCGAACTCATCGAAATGAATTCGGCTTTCTGATTTGATGTTCTACTCTAAAAGTGCGGTTAATTTCTTACTCATAAATCTTGAAGATTTACtt  >  1:792396/1‑251 (MQ=255)                                                                                                                                                                              |                                                                                                                                                                                                                                                   ATAAGGTATAAAAGGGGGTTAAACGAGTTTAACATAACCAAATATCCGCGCTCATTCCATGGTTATTTTACATTTTCTTGTTTTTGATCAATTTTTCCAATTTAATTTTCTATTTTAGTGAAATTTTGTAGCATGAAAATTTCTCCTAAAATGTGTCAACTACCTATTTATTATTATAAAAATATCATTAAAGGGGAAAATTAAATGAAGCAGGAATATATAACTATAAAGAGGTATATTTACATTTTATGCTACAAAAAAGAATTATTTTTATTTGAATATTTTATCGAAGAAGAAATACCCTAATTACGGGCAATAAAAAACCGAACTCATCGAAATGAATTCGGCTTTCTGATTTGATGTTCTACTCTAAAAGTGCGGTTAATTTCTTACTCATAAATCTTGAAGATTTACTT  >  1/18093‑18508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑