Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,204,181	C→A	intergenic (+44601/+216756)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,204,181	0	C	A	100.0%	143.7 / NA	53	intergenic (+44601/+216756)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/26);  total (27/26)

TTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTTCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTCTGATTGAAGCGTTCAAGCGTTACTACCCTACTCTTACGCTGGATGCCATCCTGGGAAATTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAAATGGGCTTGATGGAAGAAGAAAACGGCATTATCGTCGGCAAGCTCGGCAAAAAACTTTTACGCTTTATCGGTGCGCAATTCGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGAC  >  1/1203939‑1204421                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ttCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa                                                                                                                                                                                                                                          <  1:476304/251‑1 (MQ=255)         aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaa                                                                                                                                                                                                                                  >  1:248688/1‑251 (MQ=255)         aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGCGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaa                                                                                                                                                                                                                                  >  1:679974/1‑251 (MQ=255)                   atgatgAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaa                                                                                                                                                                                                                                <  1:785354/243‑2 (MQ=255)                       tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                                                                                                                                                                                                    <  1:665924/251‑14 (MQ=255)                       tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                                                                                                                                                                                                    <  1:504395/251‑14 (MQ=255)                       tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                                                                                                                                                                                                    <  1:342106/251‑14 (MQ=255)                           aaaaGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGTAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACg                                                                                                                                                                                                                <  1:275186/251‑1 (MQ=255)                                   atatTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAAttt                                                                                                                                                                                                        >  1:106068/1‑251 (MQ=255)                                        gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCaacc                                                                                                                                                                                                                                       <  1:147112/215‑4 (MQ=255)                                        gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGcca                                                                                                                                                                                                    >  1:492742/1‑248 (MQ=255)                                        gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGcca                                                                                                                                                                                                    >  1:724521/1‑248 (MQ=255)                                           ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                                                                                                                                                                                                    >  1:322697/1‑218 (MQ=255)                                           ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaa                                                                                                                                                                                                >  1:820065/1‑245 (MQ=255)                                           ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACATaaaaaa                                                                                                                                                                                                                                <  1:839431/219‑2 (MQ=255)                                                   gTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGGGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag                                                                                                                                                                                        >  1:438044/1‑237 (MQ=255)                                                   gTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag                                                                                                                                                                                        >  1:102095/1‑237 (MQ=255)                                                    tttGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag                                                                                                                                                                                        <  1:175940/250‑15 (MQ=255)                                                      tGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtac                                                                                                                                                                                     <  1:377257/251‑18 (MQ=255)                                                        tCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtacaa                                                                                                                                                                                   <  1:339015/251‑20 (MQ=255)                                                           cTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTAt                                                                                                                                                                                                                                            <  1:797784/191‑1 (MQ=255)                                                           cTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtacaaaaa                                                                                                                                                                                >  1:878219/1‑229 (MQ=255)                                                                       cGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaa                                                                                                                                                                                                                                 <  1:802996/190‑1 (MQ=255)                                                                           ttttCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaa                                                                                                                                                                                                                                 >  1:160864/1‑186 (MQ=255)                                                                                 aaGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa                                                                                                                                                                                                                                          >  1:294301/1‑171 (MQ=255)                                                                                           ttACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTAGATGCCAATTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTAt                                                                                                                                                                                                                                            <  1:388841/159‑1 (MQ=255)                                                                                                                                                                                 ttATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCg                                                          <  1:637904/251‑1 (MQ=255)                                                                                                                                                                                 ttATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCg                                                          <  1:561656/251‑1 (MQ=255)                                                                                                                                                                                         aaaatatggcagtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGa                                                    <  1:30421/229‑1 (MQ=255)                                                                                                                                                                                         aaaatatggcagtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGa                                                    <  1:37004/229‑1 (MQ=255)                                                                                                                                                                                              atggcagtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTa                                              <  1:213856/235‑1 (MQ=255)                                                                                                                                                                                                    gtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCaa                                        >  1:397716/11‑251 (MQ=255)                                                                                                                                                                                                        ctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCg                                                          >  1:665929/7‑229 (MQ=255)                                                                                                                                                                                                          ccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGc                                                           >  1:689161/5‑226 (MQ=255)                                                                                                                                                                                                                  gttttgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATgg                          >  1:89452/24‑251 (MQ=255)                                                                                                                                                                                                                    tttgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGCAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGa                        <  1:640863/230‑1 (MQ=255)                                                                                                                                                                                                                      tgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAAc                      <  1:271261/232‑1 (MQ=255)                                                                                                                                                                                                                      tgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAAc                      <  1:801555/232‑1 (MQ=255)                                                                                                                                                                                                                       gtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGa                        >  1:495319/19‑248 (MQ=255)                                                                                                                                                                                                                          gtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGc                                                           >  1:703339/16‑210 (MQ=255)                                                                                                                                                                                                                            gagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAAt                   >  1:118435/14‑248 (MQ=255)                                                                                                                                                                                                                            gagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCt                 >  1:459109/14‑250 (MQ=255)                                                                                                                                                                                                                              gcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGAt                                                   >  1:806579/12‑214 (MQ=255)                                                                                                                                                                                                                                ttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAGACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACgggg                                                                     <  1:643501/185‑1 (MQ=255)                                                                                                                                                                                                                                ttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTaaa                                                              <  1:783674/192‑1 (MQ=255)                                                                                                                                                                                                                                ttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGt            <  1:185175/242‑1 (MQ=255)                                                                                                                                                                                                                                 tactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAAc                      >  1:830119/9‑240 (MQ=255)                                                                                                                                                                                                                                 tactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGtt           >  1:617744/9‑251 (MQ=255)                                                                                                                                                                                                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATc                  >  1:205277/1‑235 (MQ=255)                                                                                                                                                                                                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGa   >  1:173188/1‑250 (MQ=255)                                                                                                                                                                                                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGa   >  1:874140/1‑250 (MQ=255)                                                                                                                                                                                                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGa   >  1:53841/1‑250 (MQ=255)                                                                                                                                                                                                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGAc  <  1:332126/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 TTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTTCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTCTGATTGAAGCGTTCAAGCGTTACTACCCTACTCTTACGCTGGATGCCATCCTGGGAAATTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAAATGGGCTTGATGGAAGAAGAAAACGGCATTATCGTCGGCAAGCTCGGCAAAAAACTTTTACGCTTTATCGGTGCGCAATTCGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGAC  >  1/1203939‑1204421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑