Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,204,091	T→A	intergenic (+44511/+216846)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,204,091	0	T	A	100.0%	187.9 / NA	68	intergenic (+44511/+216846)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (33/35);  total (33/35)

CAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTTCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTCTGATTGAAGCGTTCAAGCGTTACTACCCTACTCTTACGCTGGATGCCATCCTGGGAAATTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAA  >  1/1203844‑1204247                                                                                                                                                                                                                                                        |                                                                                                                                                              caacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACg                                                                                                                                                            >  1:9114/1‑251 (MQ=255) caacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACg                                                                                                                                                            >  1:428028/1‑251 (MQ=255)  aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:78874/251‑1 (MQ=255)  aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:160197/251‑1 (MQ=255)  aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:316111/251‑1 (MQ=255)        cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATg                                                                                                                                                     >  1:597030/1‑251 (MQ=255)        cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATg                                                                                                                                                     >  1:532029/1‑251 (MQ=255)              atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAttt                                                                                                                                               >  1:751286/1‑249 (MQ=255)              atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAttt                                                                                                                                               <  1:253896/251‑3 (MQ=255)                 ttattGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:712633/236‑1 (MQ=255)                 ttattGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttggg                                                                                                                                            >  1:177848/1‑246 (MQ=255)                   attGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           >  1:207709/1‑234 (MQ=255)                   attGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:415719/234‑1 (MQ=255)                        tCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttggggtaatta                                                                                                                                     <  1:9967/251‑13 (MQ=255)                           tGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttggggtaattatc                                                                                                                                   >  1:161612/1‑236 (MQ=255)                            gTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCg                                                                                                                                 >  1:30557/1‑251 (MQ=255)                              tACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGaa                                                                                                                               <  1:581010/251‑1 (MQ=255)                                ccAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGaaaa                                                                                                                             >  1:320157/1‑251 (MQ=255)                                   aCAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAAtat                                                                                                                          >  1:361354/1‑249 (MQ=255)                                      gTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAAt                                                                                                                            >  1:782245/1‑246 (MQ=255)                                      gTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATgg                                                                                                                        >  1:642513/1‑250 (MQ=255)                                                cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctcct                                                                                                             <  1:543871/251‑10 (MQ=255)                                                cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctc                                                                                                               >  1:318898/1‑242 (MQ=255)                                                cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctc                                                                                                               >  1:344569/1‑242 (MQ=255)                                                      acacATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctatccatattag                                                                                                       <  1:869315/251‑16 (MQ=255)                                                       cacaTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGTATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAGGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagt                                                                                                      <  1:443342/251‑17 (MQ=255)                                                       cacaTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           >  1:900208/1‑198 (MQ=255)                                                       cacaTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:82953/198‑1 (MQ=255)                                                         caTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTGATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttt                                                                                                    >  1:463098/1‑233 (MQ=255)                                                         caTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttt                                                                                                    >  1:786281/1‑233 (MQ=255)                                                              cGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttttgtag                                                                                               <  1:486176/251‑24 (MQ=255)                                                              cGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttttgtag                                                                                               <  1:392351/251‑24 (MQ=255)                                                                  aaTCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctcc                                                                                                              <  1:276380/232‑9 (MQ=255)                                                                     cTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagt                                                                                                      >  1:314391/1‑221 (MQ=255)                                                                                      cTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAAc                                                                      <  1:366699/251‑1 (MQ=255)                                                                                                ttCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa                                                            <  1:476304/251‑1 (MQ=255)                                                                                                        aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGc                                                                                                                       <  1:93268/185‑1 (MQ=255)                                                                                                        aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaa                                                    >  1:248688/1‑251 (MQ=255)                                                                                                        aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGCGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaa                                                    >  1:679974/1‑251 (MQ=255)                                                                                                              gtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttatt                                                                                                         <  1:317060/193‑14 (MQ=255)                                                                                                                  atgatgAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattag                                                                                                       <  1:326821/191‑16 (MQ=255)                                                                                                                  atgatgAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaa                                                  <  1:785354/243‑2 (MQ=255)                                                                                                                      tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      <  1:665924/251‑14 (MQ=255)                                                                                                                      tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      <  1:504395/251‑14 (MQ=255)                                                                                                                      tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      <  1:342106/251‑14 (MQ=255)                                                                                                                          aaaaGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGTAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACg                                  <  1:275186/251‑1 (MQ=255)                                                                                                                             aGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttgggg                                                                                                                                           >  1:521336/1‑138 (MQ=255)                                                                                                                             aGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctcc                                                                                                              <  1:120654/173‑9 (MQ=255)                                                                                                                                cAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAAt                                                                                                                            <  1:220848/156‑1 (MQ=255)                                                                                                                                  atatTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattag                                                                                                       >  1:229109/1‑160 (MQ=255)                                                                                                                                  atatTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAAttt                          >  1:106068/1‑251 (MQ=255)                                                                                                                                       gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCaacc                                                         <  1:147112/215‑4 (MQ=255)                                                                                                                                       gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGcca                      >  1:724521/1‑248 (MQ=255)                                                                                                                                       gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGcca                      >  1:492742/1‑248 (MQ=255)                                                                                                                                          ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      >  1:322697/1‑218 (MQ=255)                                                                                                                                          ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaa                  >  1:820065/1‑245 (MQ=255)                                                                                                                                          ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACATaaaaaa                                                  <  1:839431/219‑2 (MQ=255)                                                                                                                                                  gTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGGGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag          >  1:438044/1‑237 (MQ=255)                                                                                                                                                  gTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag          >  1:102095/1‑237 (MQ=255)                                                                                                                                                   tttGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag          <  1:175940/250‑15 (MQ=255)                                                                                                                                                     tGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtac       <  1:377257/251‑18 (MQ=255)                                                                                                                                                       tCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtacaa     <  1:339015/251‑20 (MQ=255)                                                                                                                                                          cTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTAt                                                              <  1:797784/191‑1 (MQ=255)                                                                                                                                                          cTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtacaaaaa  >  1:878219/1‑229 (MQ=255)                                                                                                                                                                      cGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaa                                                   <  1:802996/190‑1 (MQ=255)                                                                                                                                                                          ttttCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaa                                                   >  1:160864/1‑186 (MQ=255)                                                                                                                                                                                aaGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa                                                            >  1:294301/1‑171 (MQ=255)                                                                                                                                                                                          ttACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTAGATGCCAATTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTAt                                                              <  1:388841/159‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                              CAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTTCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTCTGATTGAAGCGTTCAAGCGTTACTACCCTACTCTTACGCTGGATGCCATCCTGGGAAATTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAA  >  1/1203844‑1204247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑