Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,204,076	T→C	intergenic (+44496/+216861)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,204,076	0	T	C	100.0%	219.0 / NA	78	intergenic (+44496/+216861)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/41);  total (37/41)

CCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTTCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTCTGATTGAAGCGTTCAAGCGTTACTACCCTACTCTTACGCTGGATGCCATCCTGGGAAATTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAA  >  1/1203834‑1204247                                                                                                                                                                                                                                                   |                                                                                                                                                                             ccGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                                                                                      <  1:245743/251‑1 (MQ=255) ccGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                                                                                      <  1:825594/251‑1 (MQ=255)   gTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCtactac                                                                                                                                                                        <  1:806219/247‑1 (MQ=255)   gTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTa                                                                                                                                                                    >  1:97979/1‑251 (MQ=255)      tattaCAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTActc                                                                                                                                                                 <  1:220782/251‑1 (MQ=255)           caacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACg                                                                                                                                                            >  1:428028/1‑251 (MQ=255)           caacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACg                                                                                                                                                            >  1:9114/1‑251 (MQ=255)            aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:316111/251‑1 (MQ=255)            aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:160197/251‑1 (MQ=255)            aacaaACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:78874/251‑1 (MQ=255)                  cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTActctaa                                                                                                                                                              <  1:119886/242‑3 (MQ=255)                  cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATg                                                                                                                                                     >  1:597030/1‑251 (MQ=255)                  cGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATg                                                                                                                                                     >  1:532029/1‑251 (MQ=255)                        atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTAc                                                                                                                                                                   >  1:286458/1‑231 (MQ=255)                        atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAttt                                                                                                                                               <  1:253896/251‑3 (MQ=255)                        atatTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAttt                                                                                                                                               >  1:751286/1‑249 (MQ=255)                           ttattGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:712633/236‑1 (MQ=255)                           ttattGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttggg                                                                                                                                            >  1:177848/1‑246 (MQ=255)                             attGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           >  1:207709/1‑234 (MQ=255)                             attGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:415719/234‑1 (MQ=255)                                  tCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttggggtaatta                                                                                                                                     <  1:9967/251‑13 (MQ=255)                                     tGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttggggtaattatc                                                                                                                                   >  1:161612/1‑236 (MQ=255)                                      gTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCg                                                                                                                                 >  1:30557/1‑251 (MQ=255)                                        tACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGaa                                                                                                                               <  1:581010/251‑1 (MQ=255)                                          ccAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGaaaa                                                                                                                             >  1:320157/1‑251 (MQ=255)                                             aCAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAAtat                                                                                                                          >  1:361354/1‑249 (MQ=255)                                                gTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAAt                                                                                                                            >  1:782245/1‑246 (MQ=255)                                                gTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATgg                                                                                                                        >  1:642513/1‑250 (MQ=255)                                                          cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctcct                                                                                                             <  1:543871/251‑10 (MQ=255)                                                          cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctc                                                                                                               >  1:318898/1‑242 (MQ=255)                                                          cGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctc                                                                                                               >  1:344569/1‑242 (MQ=255)                                                                acacATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctatccatattag                                                                                                       <  1:869315/251‑16 (MQ=255)                                                                 cacaTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGTATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAGGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagt                                                                                                      <  1:443342/251‑17 (MQ=255)                                                                 cacaTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           <  1:82953/198‑1 (MQ=255)                                                                 cacaTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGc                                                                                                                                                           >  1:900208/1‑198 (MQ=255)                                                                   caTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTGATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttt                                                                                                    >  1:463098/1‑233 (MQ=255)                                                                   caTGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttt                                                                                                    >  1:786281/1‑233 (MQ=255)                                                                        cGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttttgtag                                                                                               <  1:392351/251‑24 (MQ=255)                                                                        cGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagttttgtag                                                                                               <  1:486176/251‑24 (MQ=255)                                                                            aaTCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctcc                                                                                                              <  1:276380/232‑9 (MQ=255)                                                                               cTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattagt                                                                                                      >  1:314391/1‑221 (MQ=255)                                                                                                cTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAAc                                                                      <  1:366699/251‑1 (MQ=255)                                                                                                          ttCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa                                                            <  1:476304/251‑1 (MQ=255)                                                                                                                  aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                                                                                      >  1:592138/1‑138 (MQ=255)                                                                                                                  aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                                                                                      >  1:531420/1‑138 (MQ=255)                                                                                                                  aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGc                                                                                                                       <  1:93268/185‑1 (MQ=255)                                                                                                                  aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaa                                                    >  1:248688/1‑251 (MQ=255)                                                                                                                  aggtaggtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGCGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaa                                                    >  1:679974/1‑251 (MQ=255)                                                                                                                        gtTAATGATGAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttatt                                                                                                         <  1:317060/193‑14 (MQ=255)                                                                                                                            atgatgAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattag                                                                                                       <  1:326821/191‑16 (MQ=255)                                                                                                                            atgatgAAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaa                                                  <  1:785354/243‑2 (MQ=255)                                                                                                                                tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      <  1:504395/251‑14 (MQ=255)                                                                                                                                tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      <  1:665924/251‑14 (MQ=255)                                                                                                                                tgaAAAAAGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      <  1:342106/251‑14 (MQ=255)                                                                                                                                    aaaaGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGTAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACg                                  <  1:275186/251‑1 (MQ=255)                                                                                                                                       aGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCAtttgggg                                                                                                                                           >  1:521336/1‑138 (MQ=255)                                                                                                                                       aGCCAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctcc                                                                                                              <  1:120654/173‑9 (MQ=255)                                                                                                                                          cAATATTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAAt                                                                                                                            <  1:220848/156‑1 (MQ=255)                                                                                                                                            atatTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCagtctctccttattag                                                                                                       >  1:229109/1‑160 (MQ=255)                                                                                                                                            atatTGCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAAttt                          >  1:106068/1‑251 (MQ=255)                                                                                                                                                 gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCaacc                                                         <  1:147112/215‑4 (MQ=255)                                                                                                                                                 gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGcca                      >  1:492742/1‑248 (MQ=255)                                                                                                                                                 gCATTATTCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGcca                      >  1:724521/1‑248 (MQ=255)                                                                                                                                                    ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaaatccctttatggt                                      >  1:322697/1‑218 (MQ=255)                                                                                                                                                    ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaa                  >  1:820065/1‑245 (MQ=255)                                                                                                                                                    ttattCCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACATaaaaaa                                                  <  1:839431/219‑2 (MQ=255)                                                                                                                                                            gTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGGGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag          >  1:438044/1‑237 (MQ=255)                                                                                                                                                            gTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag          >  1:102095/1‑237 (MQ=255)                                                                                                                                                             tttGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaag          <  1:175940/250‑15 (MQ=255)                                                                                                                                                               tGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtac       <  1:377257/251‑18 (MQ=255)                                                                                                                                                                 tCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtacaa     <  1:339015/251‑20 (MQ=255)                                                                                                                                                                    cTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTAt                                                              <  1:797784/191‑1 (MQ=255)                                                                                                                                                                    cTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGccacaaaatcagaagtacaaaaa  >  1:878219/1‑229 (MQ=255)                                                                                                                                                                                cGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaa                                                   <  1:802996/190‑1 (MQ=255)                                                                                                                                                                                    ttttCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTaaaaa                                                   >  1:160864/1‑186 (MQ=255)                                                                                                                                                                                      ttCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTAccc                                                                                                                                                                      <  1:890207/70‑1 (MQ=255)                                                                                                                                                                                          aaGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTGGATGCCATTTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCa                                                            >  1:294301/1‑171 (MQ=255)                                                                                                                                                                                                    ttACTTCTACTGGCAAATAAAATTCGTTTAATCGAAGCATTTAAGCGCTACTACCCTACTCTAACGCTAGATGCCAATTGGGGTAATTATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTAt                                                              <  1:388841/159‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                             CCGTTTATTACAACAAACGATAGATATTATTGTTCATGTTACCAACAGTCATGAACACGGCAGACACATGACGGAAATCTATTTTGATCCGCAAGCTAAACTGAATTCGCTCAAGGTAGGTTAATGATGAAAAAAGCCAATATTGCATTATTTCTGTTTGTCACTTTGCTTCCGACGTATTTTCTAAGTGGGCTTTTACTTCTACTGGCAAATAAAATTCGTCTGATTGAAGCGTTCAAGCGTTACTACCCTACTCTTACGCTGGATGCCATCCTGGGAAATTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAA  >  1/1203834‑1204247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑