Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	248,818	A→G	intergenic (+115507/+13413)	unknown → / ← unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	248,818	0	A	G	100.0%	88.8 / NA	32	intergenic (+115507/+13413)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

CCGGATAAGTTATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGATATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGAAAAACTTCGGCGTGAGTCCCGGCACGATTCATGTGCGGGTGGAAAAAATGCGTCAATCCGGTGTAATTGAA  >  1/248571‑249037                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                            ccGGATAAGTTATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGgtat                                                                                                                                                                                                                          >  1:652524/1‑251 (MQ=255)       aaGTTATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTAttt                                                                                                                                                                                                                     >  1:191309/1‑250 (MQ=255)       aaGTTATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTa                                                                                                                                                                                                                    >  1:195436/1‑251 (MQ=255)        aGTTATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAg                                                                                                                                                                                                                   >  1:512983/1‑251 (MQ=255)           tATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATg                                                                                                                                                                                                                <  1:192824/251‑1 (MQ=255)           tATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGGGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATg                                                                                                                                                                                                                <  1:440574/251‑1 (MQ=255)               ttGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGAt                                                                                                                                                                                                                 >  1:174697/1‑246 (MQ=255)                   aTACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATaa                                                                                                                                                                                                        <  1:104654/251‑1 (MQ=255)                    tACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAAt                                                                                                                                                                                                       <  1:536310/251‑1 (MQ=255)                    tACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAAt                                                                                                                                                                                                       <  1:30156/251‑1 (MQ=255)                      ccATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAAttt                                                                                                                                                                                                     >  1:377334/1‑251 (MQ=255)                         ttACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAAt                                                                                                                                                                                                       <  1:130734/246‑1 (MQ=255)                                    gCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGGGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATg                                                                                                                                                                                                                <  1:431933/226‑1 (MQ=255)                                           aTAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAata                                                                                                                                                                                <  1:726014/251‑1 (MQ=255)                                               gTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTAttt                                                                                                                                                                                                                     <  1:898062/210‑1 (MQ=255)                                                tCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAAt                                                                                                                                                                                                       <  1:719923/223‑1 (MQ=255)                                                tCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATAttttt                                                                                                                                                                           >  1:554380/1‑251 (MQ=255)                                                        cTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAgg                                                                                                                                                                   <  1:2142/251‑1 (MQ=255)                                                               aatGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTCTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATAc                                                                                                                                                            <  1:424881/251‑1 (MQ=255)                                                                atGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATAc                                                                                                                                                            >  1:303754/1‑250 (MQ=255)                                                                 tGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTa                                                                                                                                                          <  1:594152/251‑1 (MQ=255)                                                                    cAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGc                                                                                                                                                       <  1:763344/251‑1 (MQ=255)                                                                             cAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATg                                                                                                                                                                                                                >  1:487798/1‑185 (MQ=255)                                                                                                                                                     tCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGaaaa                                                                      >  1:727592/1‑251 (MQ=255)                                                                                                                                                                cTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATAc                                                                                                                                                            <  1:157785/154‑1 (MQ=255)                                                                                                                                                                               aaGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAatat                                                                                                                                                                                                          <  1:503742/93‑1 (MQ=255)                                                                                                                                                                                       gcgcTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTc                                                                                                                                                                                                 >  1:267857/1‑94 (MQ=255)                                                                                                                                                                                                              aaCTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATAc                                                                                                                                                            <  1:376039/108‑1 (MQ=255)                                                                                                                                                                                                               aCTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAAt                                                                                                                                                                                                       >  1:639179/1‑64 (MQ=255)                                                                                                                                                                                                                       cAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGAAAAACTTCGGCGTGAGTCCCGGCACGATTCATGTGCGGGTGGAAAAAATGCGTCAATCCGGTGTAATTg    <  1:369324/251‑1 (MQ=255)                                                                                                                                                                                                                       cAATATAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGAAAAACTTCGGCGTGAGTCCCGGCACGATTCATGTGCGGGTGGAAAAAATGCGTCAATCCGGTGTAATTg    >  1:806271/1‑251 (MQ=255)                                                                                                                                                                                                                         atatAATTCGACAAAAACAAATATAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGAAAAACTTCGGCGTGAGTCCCGGCACGATTCATGTGCGGGTGGAAAAAATGCGTCAATCCGGTGTAATTGaa  >  1:343568/1‑251 (MQ=255)                                                                                                                                                                                                                                             atatAAAACTGGTATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGAAAAACTTCGGCGTGAGTCCCGGCACGATTCATGTGCGGGTGGAAAAAATGCGTCAATCCGGTGTAATTg    <  1:280695/229‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                            CCGGATAAGTTATCTTGCATACCATTACCCACTTGGCTTAAGATAGGTCCCTGAACTTCAATAATGCCAAGTTGTTCAATAAGATTTTGTGTGAATGTGTTTTTCACAAAGCTGATTTCTTGTTGCTGTAAAATAAATGTCTTTTTCATCGTTATACCCCTATTTTTTCATTTAAAGTAACGGCGCTGATTTTGCATATTCATCAAACTTAATGCAATATAATTCGACAAAAACAAATATAAAACTGATATTATTTAGATGAAATATAATTTATTCTTTATTTTATTAAAATATTTTTAAACAAGGAGTATACTATGCACAATATTGATAATTTAGATCAGCAAATATTGCGCGTGTTAACGCGGGATGCCCGCACGCCTTATGCGGAAATGGCGAAAAACTTCGGCGTGAGTCCCGGCACGATTCATGTGCGGGTGGAAAAAATGCGTCAATCCGGTGTAATTGAA  >  1/248571‑249037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑