Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,203,411	T→C	intergenic (+43831/+217526)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,203,411	0	T	C	100.0%	193.8 / NA	68	intergenic (+43831/+217526)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/30);  total (38/30)

TTATCTGCGGTATTACCGAACGGCGAGCGGACACAGATTATTCTTCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACCGGACACTACTGGAAATTTATCATAAGGGCGATTATGCGGAATTTCTTAAACAAGCGGTTATCTGCGGGAAGAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACC  >  1/1203169‑1203647                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             ttttcggcggttttaccgccgggcgggcgGACACAGATTTTTCTTCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCgg                                                                                                                                                                                                                                      <  1:184635/230‑1 (MQ=255)                 cgcccGGCGAGCGGACACAGATTATTCTCCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGAtt                                                                                                                                                                                                                      <  1:69356/247‑1 (MQ=255)                                      ttattCTTCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCggg                                                                                                                                                                                                 >  1:611625/1‑251 (MQ=255)                                      ttattCTTCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCggg                                                                                                                                                                                                 >  1:473939/1‑251 (MQ=255)                                        attCTTCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGa                                                                                                                                                                                                >  1:677345/1‑250 (MQ=255)                                           ctcccgcccgcCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAAc                                                                                                                                                                                            <  1:603971/248‑1 (MQ=255)                                                     ccAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATg                                                                                                                                                                                  <  1:228276/251‑1 (MQ=255)                                                     ccAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATg                                                                                                                                                                                  >  1:234183/1‑251 (MQ=255)                                                                    ccATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGAt                                                                                                                                                                   >  1:529214/1‑251 (MQ=255)                                                                        cAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCtagt                                                                                                                                                                       >  1:157689/1‑240 (MQ=255)                                                                        cAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATtata                                                                                                                                                               >  1:665791/1‑251 (MQ=255)                                                                          gTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAatat                                                                                                                                                                                                                             <  1:239679/187‑1 (MQ=255)                                                                          gTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATAtt                                                                                                                                                             >  1:235783/1‑251 (MQ=255)                                                                          gTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATAtt                                                                                                                                                             >  1:720757/1‑251 (MQ=255)                                                                           tATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATtata                                                                                                                                                               <  1:472662/248‑1 (MQ=255)                                                                           tATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTc                                                                                                                                                            <  1:148275/251‑1 (MQ=255)                                                                                cGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTg                                                                                                                                                       <  1:508718/251‑1 (MQ=255)                                                                                        aaaCCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACg                                                                                                                                               <  1:213533/251‑1 (MQ=255)                                                                                             gAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtgg                                                                                                                                          >  1:37453/1‑249 (MQ=255)                                                                                             gAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTATGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtgg                                                                                                                                          >  1:423158/1‑249 (MQ=255)                                                                                                 aaGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                      <  1:728520/251‑7 (MQ=255)                                                                                                 aaGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                      <  1:193704/251‑7 (MQ=255)                                                                                                   gTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCa                                                                                                                                    >  1:564395/1‑251 (MQ=255)                                                                                                      tttttttGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCAttg                                                                                                                                 <  1:118863/251‑3 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATg                                                                                                                                                                                  <  1:722982/190‑1 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTc                                                                                                                                                            <  1:381927/212‑1 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggacc                                                                                                                                     >  1:569426/1‑228 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATAcccc                                                                                                                       >  1:222634/1‑246 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCgg                                                                                                                     >  1:333253/1‑251 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCgg                                                                                                                     >  1:667406/1‑251 (MQ=255)                                                                                                                  ggATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCgg                                                                                                                     >  1:72941/1‑251 (MQ=255)                                                                                                                   gATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTatat                                                                                                                                                              >  1:5820/1‑209 (MQ=255)                                                                                                                     ttattaCATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTGTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACg                                                                                                                                               <  1:441469/222‑1 (MQ=255)                                                                                                                         taCATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAattatt                                                                                                              >  1:880128/1‑251 (MQ=255)                                                                                                                               aacaacGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACg                                                                                                        <  1:697527/251‑1 (MQ=255)                                                                                                                                          ttttAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                      <  1:713014/210‑7 (MQ=255)                                                                                                                                            ttAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGaac                                                                                           <  1:697758/251‑2 (MQ=255)                                                                                                                                                      tATCAAGACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACt                                                                                 <  1:82622/251‑1 (MQ=255)                                                                                                                                                            gACGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACAt                                                                                                 >  1:92988/1‑229 (MQ=255)                                                                                                                                                             aCGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTc                                                                            <  1:121596/249‑1 (MQ=255)                                                                                                                                                              cGGAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTAt                                                                         >  1:700861/1‑251 (MQ=255)                                                                                                                                                               ggAAAATCGTTTGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTc                                                                                                                                                                      <  1:876584/157‑1 (MQ=255)                                                                                                                                                                        tttGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAg                                                               >  1:63083/1‑251 (MQ=255)                                                                                                                                                                         ttGTCAGATGATGACTGGACACTACTGGAAATTCATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGc                                                              <  1:620303/251‑1 (MQ=255)                                                                                                                                                                          tGTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                      >  1:241233/1‑172 (MQ=255)                                                                                                                                                                           gTCAGATGATGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCaa                                                            >  1:834854/1‑251 (MQ=255)                                                                                                                                                                               gatgatGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCAttg                                                                                                                                 <  1:796907/178‑3 (MQ=255)                                                                                                                                                                               gatgatGACTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGaac                                                                                           >  1:843645/1‑215 (MQ=255)                                                                                                                                                                                    tgaCTGGACACTACTGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCg                                                                                                                                                                     <  1:560036/137‑1 (MQ=255)                                                                                                                                                                                              ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATc                                                                        <  1:598776/220‑1 (MQ=255)                                                                                                                                                                                              ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCa                                          >  1:467232/1‑250 (MQ=255)                                                                                                                                                                                              ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCa                                          >  1:161532/1‑250 (MQ=255)                                                                                                                                                                                              ctactGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCa                                          >  1:310402/1‑250 (MQ=255)                                                                                                                                                                                                  tGGAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATc                                                                        >  1:392256/1‑216 (MQ=255)                                                                                                                                                                                                   ggAAATTTATCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCg                                                                  <  1:282654/221‑1 (MQ=255)                                                                                                                                                                                                           aTCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCtggggac                                                                                                                                      <  1:567171/145‑7 (MQ=255)                                                                                                                                                                                                           aTCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAg                                                                                              <  1:566925/185‑1 (MQ=255)                                                                                                                                                                                                            tCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGc                                      >  1:437769/1‑240 (MQ=255)                                                                                                                                                                                                            tCATAAGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTg                            >  1:806088/1‑250 (MQ=255)                                                                                                                                                                                                                aaGGGTGATTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACACCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGaa                       >  1:437037/1‑251 (MQ=255)                                                                                                                                                                                                                       aTTATGCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTg                 >  1:420622/1‑250 (MQ=255)                                                                                                                                                                                                                            gCGGAATTCCTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCAc                                                                                  >  1:479340/1‑180 (MQ=255)                                                                                                                                                                                                                                   tccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGt                                                                                                                                                         <  1:253923/100‑1 (MQ=255)                                                                                                                                                                                                                                   tccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa    >  1:830234/3‑251 (MQ=255)                                                                                                                                                                                                                                   tccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa    >  1:637024/3‑251 (MQ=255)                                                                                                                                                                                                                                    ccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa    >  1:153121/2‑250 (MQ=255)                                                                                                                                                                                                                                    ccTTAAACAAGCGGTCATTTGCGGGAAAAATATTGAGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGaaa    >  1:614619/2‑250 (MQ=255)                                                                                                                                                                                                                                     cTTAAACAAGCGGTCATTTGCGGGAAAAATATTGTGATTGCCGGGGAATCCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTAGTCGATTATATTCCGTTGCATGAACGCCTGGGGACCATTGAAGATACCCCGGAATTATTCTTACGCCAACATAAGAACTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAAcc  <  1:682227/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             TTATCTGCGGTATTACCGAACGGCGAGCGGACACAGATTATTCTTCCGCCCGCCAGTGAACGGGAAACCATCAGTATTACGGTGCGAAAACCGAATAAGTCTTTTTTTGACCTGGATTATTACATCAACAACGGCTTTTTTAGTCGTGTTATCAAGACGGAAAATCGTTTGTCAGATGATGACCGGACACTACTGGAAATTTATCATAAGGGCGATTATGCGGAATTTCTTAAACAAGCGGTTATCTGCGGGAAGAATATTGTGATTGCCGGGGAAACCGGCTCCGGGAAAACCACTTTAATGAAAGCCCTGGTCGATTATATTCCGTTGCATGAACGCCTAGGCACCATCGAAGATACTCCGGAATTATTCTTACGCCAACATAAGAATTATTTCCACTTATTCTATCCAAGCGAAGCAAAAAATGGTGATTTAATCACGGCTGCCTCTTTGCTGAAATCTTGCCTACGAATGAAACC  >  1/1203169‑1203647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑