Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,797	T→C	intergenic (+43217/+218140)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,797	0	T	C	100.0%	150.1 / NA	53	intergenic (+43217/+218140)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/25);  total (28/25)

GTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTTCATGCCAATAAATTATTCGGAGAATTGCTCGAAGACGAAGCCATTACAAAAATTGCGATTAATCGCC  >  1/1202558‑1203031                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           acggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAAcc                                                                                                                                                                                                                                 <  1:723875‑M2/133‑1 (MQ=255)    gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCAtt                                                                                                                                                                                                                              >  1:11000‑M2/116‑251 (MQ=255)           accaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTc                                                                                                                                                                                                                       <  1:556672‑M2/143‑1 (MQ=255)                            gcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAg                                                                                                                                                                                                      >  1:397013‑M2/92‑251 (MQ=255)                                aaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAAcc                                                                                                                                                                                                  <  1:465062‑M2/164‑1 (MQ=255)                                         tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                                                                                         >  1:657763‑M2/79‑251 (MQ=255)                                         tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                                                                                         >  1:534921‑M2/79‑251 (MQ=255)                                          atttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAata                                                                                                                                                                                                                          <  1:895880‑M2/140‑1 (MQ=255)                                                                         ggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAt                                                                                                                                                         >  1:558750‑M2/47‑251 (MQ=255)                                                                          gtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                                                                                          <  1:877090‑M2/204‑1 (MQ=255)                                                                           taaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAtt                                                                                                                                                        <  1:55230‑M2/206‑1 (MQ=255)                                                                                cccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGc                                                                                                                                                   <  1:291545‑M2/211‑1 (MQ=255)                                                                                   ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                                                                                               >  1:508186‑M2/37‑251 (MQ=255)                                                                                   ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                                                                                               >  1:482138‑M2/37‑251 (MQ=255)                                                                                          cccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGa                                                                                                                                        <  1:288050‑M2/222‑1 (MQ=255)                                                                                             ggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGaa                                                                                                                                       <  1:605604‑M2/223‑1 (MQ=255)                                                                                                            actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGc                                                                                                                                                                                                              >  1:374985/10‑163 (MQ=255)                                                                                                            actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATagga                                                                                                                      <  1:248387‑M2/240‑1 (MQ=255)                                                                                                                      aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGATTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                                                                                            <  1:456171/251‑3 (MQ=255)                                                                                                                      aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                                                                                            <  1:212741‑M2/250‑3 (MQ=255)                                                                                                                      aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                                                                                            <  1:514503‑M2/250‑3 (MQ=255)                                                                                                                           atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                                                                                          >  1:157540/1‑200 (MQ=255)                                                                                                                           atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaaccatgg                                                                                                        >  1:505424/1‑244 (MQ=255)                                                                                                                              atGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                                                                                            <  1:196232/243‑3 (MQ=255)                                                                                                                                gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                                                                                                  >  1:468242/1‑251 (MQ=255)                                                                                                                                gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                                                                                                  >  1:103920/1‑251 (MQ=255)                                                                                                                                    aGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAct                                                                                               >  1:221211/1‑250 (MQ=255)                                                                                                                                     gCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                                                                                             <  1:4890/235‑2 (MQ=255)                                                                                                                                      cATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCtt                                                                                            >  1:654303/1‑251 (MQ=255)                                                                                                                                             aCCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGa                                                                                     <  1:711969/251‑1 (MQ=255)                                                                                                                                                     aaCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCaa                                                                             <  1:393779/251‑1 (MQ=255)                                                                                                                                                      aCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAt                                                                            <  1:367161/251‑1 (MQ=255)                                                                                                                                                       ccAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtt                                                                           <  1:849504/251‑1 (MQ=255)                                                                                                                                                          gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAc                                                                                                <  1:201633/227‑1 (MQ=255)                                                                                                                                                          gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca                                                                        >  1:379915/1‑249 (MQ=255)                                                                                                                                                          gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca                                                                        >  1:271678/1‑249 (MQ=255)                                                                                                                                                             cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAAt                                                                                                                                      <  1:509628/183‑1 (MQ=255)                                                                                                                                                             cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACtc                                                                     <  1:83435/248‑2 (MQ=255)                                                                                                                                                                                           catttaatccgactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCt                                        >  1:69446/15‑250 (MQ=255)                                                                                                                                                                                           catttaatccgactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCt                                        >  1:138197/15‑250 (MQ=255)                                                                                                                                                                                                     gactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAg                             >  1:491534/5‑251 (MQ=255)                                                                                                                                                                                                     gactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAg                             >  1:350983/5‑251 (MQ=255)                                                                                                                                                                                                     gactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGAGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAg                             >  1:824023/5‑251 (MQ=255)                                                                                                                                                                                                          aTACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCAtt                        >  1:361486/1‑251 (MQ=255)                                                                                                                                                                                                               gTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACtc                                                                     >  1:139382/1‑200 (MQ=255)                                                                                                                                                                                                                 aCAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACagaaa                 <  1:804701/251‑1 (MQ=255)                                                                                                                                                                                                                      ccGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACAGAAATTg              <  1:24497/249‑1 (MQ=255)                                                                                                                                                                                                                         gCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACAGAAATTGCGAtt         >  1:103241/1‑251 (MQ=255)                                                                                                                                                                                                                          ctctgAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                                                                                            >  1:123032/5‑149 (MQ=255)                                                                                                                                                                                                                                aaTTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACAGAAATTGCGATTAATCgtc  <  1:747347/251‑3 (MQ=255)                                                                                                                                                                                                                                  ttGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                                                                                             >  1:517873/1‑141 (MQ=255)                                                                                                                                                                                                                                         aaGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACg                                                                                                                                         >  1:237977/1‑107 (MQ=255)                                                                                                                                                                                                                                         aaGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                                                                                             >  1:338909/1‑134 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           GTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTTCATGCCAATAAATTATTCGGAGAATTGCTCGAAGACGAAGCCATTACAAAAATTGCGATTAATCGCC  >  1/1202558‑1203031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑