Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,778	G→T	intergenic (+43198/+218159)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,778	0	G	T	100.0%	166.2 / NA	62	intergenic (+43198/+218159)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/30);  total (32/30)

GCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTTCATGCCAATAAATTATTCGGAGAATTGCTCGAAGACGAAGCCATTACAAAAATTGCGATT  >  1/1202531‑1203024                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaa                                                                                                                                                                                                                                                     >  1:233769‑M2/146‑251 (MQ=255)  atgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaaa                                                                                                                                                                                                                                                    <  1:861862‑M2/107‑1 (MQ=255)     ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                                                                                 >  1:257774‑M2/142‑251 (MQ=255)     ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                                                                                 >  1:707011‑M2/142‑251 (MQ=255)      tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                                                                                 >  1:223166‑M2/141‑250 (MQ=255)      tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGc                                                                                                                                                                                                                                                <  1:659247‑M2/111‑1 (MQ=255)               agatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                                                                                       >  1:389129‑M2/132‑251 (MQ=255)                gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                                                                                       >  1:344739‑M2/131‑250 (MQ=255)                gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                                                                                       >  1:119187‑M2/131‑250 (MQ=255)                 atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGt                                                                                                                                                                                                                                         <  1:312015‑M2/118‑1 (MQ=255)                 atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAtcc                                                                                                                                                                                                                                     <  1:702748‑M2/122‑2 (MQ=255)                    caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAgtatcctgg                                                                                                                                                                                                                                  <  1:445681‑M2/125‑5 (MQ=255)                      actgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAaagtatcctggag                                                                                                                                                                                                                                <  1:649342‑M2/127‑7 (MQ=255)                       ctgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACaaagtatcctggaga                                                                                                                                                                                                                               >  1:100574‑M2/124‑244 (MQ=255)                            acggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAAcc                                                                                                                                                                                                                          <  1:723875‑M2/133‑1 (MQ=255)                               gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCAtt                                                                                                                                                                                                                       >  1:11000‑M2/116‑251 (MQ=255)                                      accaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTc                                                                                                                                                                                                                <  1:556672‑M2/143‑1 (MQ=255)                                                       gcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAg                                                                                                                                                                                               >  1:397013‑M2/92‑251 (MQ=255)                                                           aaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAAcc                                                                                                                                                                                           <  1:465062‑M2/164‑1 (MQ=255)                                                                    tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                                                                                  >  1:534921‑M2/79‑251 (MQ=255)                                                                    tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                                                                                  >  1:657763‑M2/79‑251 (MQ=255)                                                                     atttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAata                                                                                                                                                                                                                   <  1:895880‑M2/140‑1 (MQ=255)                                                                                                    ggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAt                                                                                                                                                  >  1:558750‑M2/47‑251 (MQ=255)                                                                                                     gtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                                                                                   <  1:877090‑M2/204‑1 (MQ=255)                                                                                                      taaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAtt                                                                                                                                                 <  1:55230‑M2/206‑1 (MQ=255)                                                                                                           cccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGc                                                                                                                                            <  1:291545‑M2/211‑1 (MQ=255)                                                                                                              ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                                                                                        >  1:508186‑M2/37‑251 (MQ=255)                                                                                                              ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                                                                                        >  1:482138‑M2/37‑251 (MQ=255)                                                                                                                     cccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGa                                                                                                                                 <  1:288050‑M2/222‑1 (MQ=255)                                                                                                                        ggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGaa                                                                                                                                <  1:605604‑M2/223‑1 (MQ=255)                                                                                                                                       actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGc                                                                                                                                                                                                       >  1:374985/10‑163 (MQ=255)                                                                                                                                       actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATagga                                                                                                               <  1:248387‑M2/240‑1 (MQ=255)                                                                                                                                                 aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGATTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                                                                                     <  1:456171/251‑3 (MQ=255)                                                                                                                                                 aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                                                                                     <  1:514503‑M2/250‑3 (MQ=255)                                                                                                                                                 aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                                                                                     <  1:212741‑M2/250‑3 (MQ=255)                                                                                                                                                      atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                                                                                   >  1:157540/1‑200 (MQ=255)                                                                                                                                                      atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaaccatgg                                                                                                 >  1:505424/1‑244 (MQ=255)                                                                                                                                                         atGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                                                                                     <  1:196232/243‑3 (MQ=255)                                                                                                                                                           gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                                                                                           >  1:468242/1‑251 (MQ=255)                                                                                                                                                           gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                                                                                           >  1:103920/1‑251 (MQ=255)                                                                                                                                                               aGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAct                                                                                        >  1:221211/1‑250 (MQ=255)                                                                                                                                                                gCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                                                                                      <  1:4890/235‑2 (MQ=255)                                                                                                                                                                 cATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCtt                                                                                     >  1:654303/1‑251 (MQ=255)                                                                                                                                                                        aCCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGa                                                                              <  1:711969/251‑1 (MQ=255)                                                                                                                                                                                aaCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCaa                                                                      <  1:393779/251‑1 (MQ=255)                                                                                                                                                                                 aCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAt                                                                     <  1:367161/251‑1 (MQ=255)                                                                                                                                                                                  ccAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtt                                                                    <  1:849504/251‑1 (MQ=255)                                                                                                                                                                                     gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAc                                                                                         <  1:201633/227‑1 (MQ=255)                                                                                                                                                                                     gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca                                                                 >  1:271678/1‑249 (MQ=255)                                                                                                                                                                                     gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca                                                                 >  1:379915/1‑249 (MQ=255)                                                                                                                                                                                        cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAAt                                                                                                                               <  1:509628/183‑1 (MQ=255)                                                                                                                                                                                        cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACtc                                                              <  1:83435/248‑2 (MQ=255)                                                                                                                                                                                                                      catttaatccgactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCt                                 >  1:69446/15‑250 (MQ=255)                                                                                                                                                                                                                      catttaatccgactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCt                                 >  1:138197/15‑250 (MQ=255)                                                                                                                                                                                                                                gactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAg                      >  1:350983/5‑251 (MQ=255)                                                                                                                                                                                                                                gactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAg                      >  1:491534/5‑251 (MQ=255)                                                                                                                                                                                                                                gactgATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGAGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAg                      >  1:824023/5‑251 (MQ=255)                                                                                                                                                                                                                                     aTACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCAtt                 >  1:361486/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACtc                                                              >  1:139382/1‑200 (MQ=255)                                                                                                                                                                                                                                            aCAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACagaaa          <  1:804701/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACAGAAATTg       <  1:24497/249‑1 (MQ=255)                                                                                                                                                                                                                                                    gCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACTCCATGCCAATAAATTATTCGGAAAATTGCTCGAAGGCGAAGCCATTACAGAAATTGCGAtt  >  1:103241/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTTCATGCCAATAAATTATTCGGAGAATTGCTCGAAGACGAAGCCATTACAAAAATTGCGATT  >  1/1202531‑1203024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑