Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,757	G→T	intergenic (+43177/+218180)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,757	0	G	T	100.0%	158.5 / NA	58	intergenic (+43177/+218180)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/31);  total (27/31)

TCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTT  >  1/1202523‑1202964                                                                                                                                                                                                                                           |                                                                                                                                                                                                                ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  <  1:846658‑M2/97‑1 (MQ=255) ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  <  1:669835‑M2/97‑1 (MQ=255) ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGc                                                                                                                                                                                                   <  1:14698‑M2/96‑1 (MQ=255)   gtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  >  1:199476‑M2/152‑248 (MQ=255)    tttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCgg                                                                                                                                                                                                >  1:564717‑M2/151‑249 (MQ=255)         gatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaa                                                                                                                                                                                         >  1:233769‑M2/146‑251 (MQ=255)          atgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaaa                                                                                                                                                                                        <  1:861862‑M2/107‑1 (MQ=255)             ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                     >  1:707011‑M2/142‑251 (MQ=255)             ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                     >  1:257774‑M2/142‑251 (MQ=255)              tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                     >  1:223166‑M2/141‑250 (MQ=255)              tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGc                                                                                                                                                                                    <  1:659247‑M2/111‑1 (MQ=255)                       agatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                           >  1:389129‑M2/132‑251 (MQ=255)                        gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                           >  1:119187‑M2/131‑250 (MQ=255)                        gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                           >  1:344739‑M2/131‑250 (MQ=255)                         atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGt                                                                                                                                                                             <  1:312015‑M2/118‑1 (MQ=255)                         atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAtcc                                                                                                                                                                         <  1:702748‑M2/122‑2 (MQ=255)                            caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  >  1:205200‑M2/127‑223 (MQ=255)                            caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAgtatcctgg                                                                                                                                                                      <  1:445681‑M2/125‑5 (MQ=255)                              actgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAaagtatcctggag                                                                                                                                                                    <  1:649342‑M2/127‑7 (MQ=255)                               ctgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACaaagtatcctggaga                                                                                                                                                                   >  1:100574‑M2/124‑244 (MQ=255)                                    acggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAAcc                                                                                                                                                              <  1:723875‑M2/133‑1 (MQ=255)                                       gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCAtt                                                                                                                                                           >  1:11000‑M2/116‑251 (MQ=255)                                              accaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTc                                                                                                                                                    <  1:556672‑M2/143‑1 (MQ=255)                                                               gcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAg                                                                                                                                   >  1:397013‑M2/92‑251 (MQ=255)                                                                   aaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAAcc                                                                                                                               <  1:465062‑M2/164‑1 (MQ=255)                                                                            tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                      >  1:657763‑M2/79‑251 (MQ=255)                                                                            tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                      >  1:534921‑M2/79‑251 (MQ=255)                                                                             atttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAata                                                                                                                                                       <  1:895880‑M2/140‑1 (MQ=255)                                                                                                            ggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAt                                                                                      >  1:558750‑M2/47‑251 (MQ=255)                                                                                                             gtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                       <  1:877090‑M2/204‑1 (MQ=255)                                                                                                              taaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAtt                                                                                     <  1:55230‑M2/206‑1 (MQ=255)                                                                                                                   cccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGc                                                                                <  1:291545‑M2/211‑1 (MQ=255)                                                                                                                      ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                            >  1:482138‑M2/37‑251 (MQ=255)                                                                                                                      ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                            >  1:508186‑M2/37‑251 (MQ=255)                                                                                                                             cccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGa                                                                     <  1:288050‑M2/222‑1 (MQ=255)                                                                                                                                ggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGaa                                                                    <  1:605604‑M2/223‑1 (MQ=255)                                                                                                                                               actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGc                                                                                                                                           >  1:374985/10‑163 (MQ=255)                                                                                                                                               actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATagga                                                   <  1:248387‑M2/240‑1 (MQ=255)                                                                                                                                                         aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGATTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                         <  1:456171/251‑3 (MQ=255)                                                                                                                                                         aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                         <  1:212741‑M2/250‑3 (MQ=255)                                                                                                                                                         aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                         <  1:514503‑M2/250‑3 (MQ=255)                                                                                                                                                              atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                       >  1:157540/1‑200 (MQ=255)                                                                                                                                                              atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaaccatgg                                     >  1:505424/1‑244 (MQ=255)                                                                                                                                                                 atGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                         <  1:196232/243‑3 (MQ=255)                                                                                                                                                                   gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                               >  1:103920/1‑251 (MQ=255)                                                                                                                                                                   gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                               >  1:468242/1‑251 (MQ=255)                                                                                                                                                                       aGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAct                            >  1:221211/1‑250 (MQ=255)                                                                                                                                                                        gCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                          <  1:4890/235‑2 (MQ=255)                                                                                                                                                                         cATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCtt                         >  1:654303/1‑251 (MQ=255)                                                                                                                                                                                aCCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGa                  <  1:711969/251‑1 (MQ=255)                                                                                                                                                                                        aaCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCaa          <  1:393779/251‑1 (MQ=255)                                                                                                                                                                                         aCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAt         <  1:367161/251‑1 (MQ=255)                                                                                                                                                                                          ccAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtt        <  1:849504/251‑1 (MQ=255)                                                                                                                                                                                             gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAc                             <  1:201633/227‑1 (MQ=255)                                                                                                                                                                                             gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca     >  1:271678/1‑249 (MQ=255)                                                                                                                                                                                             gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca     >  1:379915/1‑249 (MQ=255)                                                                                                                                                                                                cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAAt                                                                   <  1:509628/183‑1 (MQ=255)                                                                                                                                                                                                cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACtc  <  1:83435/248‑2 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                TCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTT  >  1/1202523‑1202964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑