Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,733	A→C	intergenic (+43153/+218204)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,733	0	A	C	100.0%	165.5 / NA	60	intergenic (+43153/+218204)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/32);  total (28/32)

CTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTT  >  1/1202489‑1202964                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        tctccgggtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAacgg                                                                                                                                                                                                                                   <  1:31311‑M2/64‑2 (MQ=255)  ctccgggtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAaacggg                                                                                                                                                                                                                                  >  1:443178‑M2/187‑249 (MQ=255)        gtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacatttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATtagccaaacgggggacat                                                                                                                                                                                                                            >  1:204564‑M2/181‑243 (MQ=255)          actatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAattagccaaacgggggacattt                                                                                                                                                                                                                          <  1:312825‑M2/73‑11 (MQ=255)             atcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACtcaattagccaaacgggggacatttaat                                                                                                                                                                                                                       >  1:461564‑M2/176‑238 (MQ=255)                                   ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  <  1:846658‑M2/97‑1 (MQ=255)                                   ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  <  1:669835‑M2/97‑1 (MQ=255)                                   ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGc                                                                                                                                                                                                   <  1:14698‑M2/96‑1 (MQ=255)                                     gtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  >  1:199476‑M2/152‑248 (MQ=255)                                      tttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCgg                                                                                                                                                                                                >  1:564717‑M2/151‑249 (MQ=255)                                           gatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaa                                                                                                                                                                                         >  1:233769‑M2/146‑251 (MQ=255)                                            atgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaaa                                                                                                                                                                                        <  1:861862‑M2/107‑1 (MQ=255)                                               ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                     >  1:707011‑M2/142‑251 (MQ=255)                                               ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                     >  1:257774‑M2/142‑251 (MQ=255)                                                tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                     >  1:223166‑M2/141‑250 (MQ=255)                                                tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGc                                                                                                                                                                                    <  1:659247‑M2/111‑1 (MQ=255)                                                         agatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                           >  1:389129‑M2/132‑251 (MQ=255)                                                          gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                           >  1:344739‑M2/131‑250 (MQ=255)                                                          gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                           >  1:119187‑M2/131‑250 (MQ=255)                                                           atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGt                                                                                                                                                                             <  1:312015‑M2/118‑1 (MQ=255)                                                           atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAtcc                                                                                                                                                                         <  1:702748‑M2/122‑2 (MQ=255)                                                              caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                                  >  1:205200‑M2/127‑223 (MQ=255)                                                              caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAgtatcctgg                                                                                                                                                                      <  1:445681‑M2/125‑5 (MQ=255)                                                                actgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCcaaacggggg                                                                                                                                                                                                                                >  1:299954‑M2/125‑187 (MQ=255)                                                                actgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAaagtatcctggag                                                                                                                                                                    <  1:649342‑M2/127‑7 (MQ=255)                                                                 ctgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACaaagtatcctggaga                                                                                                                                                                   >  1:100574‑M2/124‑244 (MQ=255)                                                                      acggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAAcc                                                                                                                                                              <  1:723875‑M2/133‑1 (MQ=255)                                                                         gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCAtt                                                                                                                                                           >  1:11000‑M2/116‑251 (MQ=255)                                                                                accaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTc                                                                                                                                                    <  1:556672‑M2/143‑1 (MQ=255)                                                                                                 gcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAg                                                                                                                                   >  1:397013‑M2/92‑251 (MQ=255)                                                                                                     aaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAAcc                                                                                                                               <  1:465062‑M2/164‑1 (MQ=255)                                                                                                              tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                      >  1:534921‑M2/79‑251 (MQ=255)                                                                                                              tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                      >  1:657763‑M2/79‑251 (MQ=255)                                                                                                               atttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAata                                                                                                                                                       <  1:895880‑M2/140‑1 (MQ=255)                                                                                                                                              ggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAt                                                                                      >  1:558750‑M2/47‑251 (MQ=255)                                                                                                                                               gtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                       <  1:877090‑M2/204‑1 (MQ=255)                                                                                                                                                taaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAtt                                                                                     <  1:55230‑M2/206‑1 (MQ=255)                                                                                                                                                     cccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGc                                                                                <  1:291545‑M2/211‑1 (MQ=255)                                                                                                                                                        ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                            >  1:482138‑M2/37‑251 (MQ=255)                                                                                                                                                        ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                            >  1:508186‑M2/37‑251 (MQ=255)                                                                                                                                                               cccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGa                                                                     <  1:288050‑M2/222‑1 (MQ=255)                                                                                                                                                                  ggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGaa                                                                    <  1:605604‑M2/223‑1 (MQ=255)                                                                                                                                                                                 actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGc                                                                                                                                           >  1:374985/10‑163 (MQ=255)                                                                                                                                                                                 actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATagga                                                   <  1:248387‑M2/240‑1 (MQ=255)                                                                                                                                                                                           aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGATTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                         <  1:456171/251‑3 (MQ=255)                                                                                                                                                                                           aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                         <  1:212741‑M2/250‑3 (MQ=255)                                                                                                                                                                                           aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                         <  1:514503‑M2/250‑3 (MQ=255)                                                                                                                                                                                                atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                       >  1:157540/1‑200 (MQ=255)                                                                                                                                                                                                atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaaccatgg                                     >  1:505424/1‑244 (MQ=255)                                                                                                                                                                                                   atGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                         <  1:196232/243‑3 (MQ=255)                                                                                                                                                                                                     gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                               >  1:103920/1‑251 (MQ=255)                                                                                                                                                                                                     gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                               >  1:468242/1‑251 (MQ=255)                                                                                                                                                                                                         aGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAct                            >  1:221211/1‑250 (MQ=255)                                                                                                                                                                                                          gCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                          <  1:4890/235‑2 (MQ=255)                                                                                                                                                                                                           cATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCtt                         >  1:654303/1‑251 (MQ=255)                                                                                                                                                                                                                  aCCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGa                  <  1:711969/251‑1 (MQ=255)                                                                                                                                                                                                                          aaCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCaa          <  1:393779/251‑1 (MQ=255)                                                                                                                                                                                                                           aCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAt         <  1:367161/251‑1 (MQ=255)                                                                                                                                                                                                                            ccAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtt        <  1:849504/251‑1 (MQ=255)                                                                                                                                                                                                                               gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAc                             <  1:201633/227‑1 (MQ=255)                                                                                                                                                                                                                               gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca     >  1:271678/1‑249 (MQ=255)                                                                                                                                                                                                                               gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca     >  1:379915/1‑249 (MQ=255)                                                                                                                                                                                                                                  cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAAt                                                                   <  1:509628/183‑1 (MQ=255)                                                                                                                                                                                                                                  cattATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAATTTCACtc  <  1:83435/248‑2 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        CTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTACTT  >  1/1202489‑1202964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑