Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,716	C→T	intergenic (+43136/+218221)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,716	0	C	T	100.0%	193.0 / NA	71	intergenic (+43136/+218221)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/37);  total (34/37)

TCTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTA  >  1/1202488‑1202961                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gtctccgggtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATc                                                                                                                                                                                                                                                   >  1:317537‑M2/189‑233 (MQ=255)  tctccgggtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAacgg                                                                                                                                                                                                                                <  1:31311‑M2/64‑2 (MQ=255)   ctccgggtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAaacggg                                                                                                                                                                                                                               >  1:443178‑M2/187‑249 (MQ=255)         gtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCt                                                                                                                                                                                                                                                  <  1:95516‑M2/46‑1 (MQ=255)         gtactatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacatttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATtagccaaacgggggacat                                                                                                                                                                                                                         >  1:204564‑M2/181‑243 (MQ=255)           actatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTAt                                                                                                                                                                                                                                                    >  1:628103‑M2/179‑222 (MQ=255)           actatcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAattagccaaacgggggacattt                                                                                                                                                                                                                       <  1:312825‑M2/73‑11 (MQ=255)              atcgatggcgaaacgttacaagccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACtcaattagccaaacgggggacatttaat                                                                                                                                                                                                                    >  1:461564‑M2/176‑238 (MQ=255)                                    ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                               <  1:669835‑M2/97‑1 (MQ=255)                                    ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                               <  1:846658‑M2/97‑1 (MQ=255)                                    ccgtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGc                                                                                                                                                                                                <  1:14698‑M2/96‑1 (MQ=255)                                      gtttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                               >  1:199476‑M2/152‑248 (MQ=255)                                       tttcagatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCgg                                                                                                                                                                                             >  1:564717‑M2/151‑249 (MQ=255)                                            gatgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaa                                                                                                                                                                                      >  1:233769‑M2/146‑251 (MQ=255)                                             atgttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGaaa                                                                                                                                                                                     <  1:861862‑M2/107‑1 (MQ=255)                                                ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                  >  1:707011‑M2/142‑251 (MQ=255)                                                ttcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                  >  1:257774‑M2/142‑251 (MQ=255)                                                 tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTg                                                                                                                                                                                  >  1:223166‑M2/141‑250 (MQ=255)                                                 tcagaatacagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGc                                                                                                                                                                                 <  1:659247‑M2/111‑1 (MQ=255)                                                         cagatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAAtt                                                                                                                                                                                                                                          <  1:755700‑M2/54‑1 (MQ=255)                                                          agatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                        >  1:389129‑M2/132‑251 (MQ=255)                                                           gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAgcc                                                                                                                                                                                                                                      <  1:611436‑M2/58‑2 (MQ=255)                                                           gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                        >  1:344739‑M2/131‑250 (MQ=255)                                                           gatacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAt                                                                                                                                                                        >  1:119187‑M2/131‑250 (MQ=255)                                                            atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGt                                                                                                                                                                          <  1:312015‑M2/118‑1 (MQ=255)                                                            atacaactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTAtcc                                                                                                                                                                      <  1:702748‑M2/122‑2 (MQ=255)                                                               caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGcc                                                                                                                                                                                               >  1:205200‑M2/127‑223 (MQ=255)                                                               caactgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAgtatcctgg                                                                                                                                                                   <  1:445681‑M2/125‑5 (MQ=255)                                                                 actgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCcaaacggggg                                                                                                                                                                                                                             >  1:299954‑M2/125‑187 (MQ=255)                                                                 actgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAaagtatcctggag                                                                                                                                                                 <  1:649342‑M2/127‑7 (MQ=255)                                                                  ctgacacggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACaaagtatcctggaga                                                                                                                                                                >  1:100574‑M2/124‑244 (MQ=255)                                                                       acggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAAcc                                                                                                                                                           <  1:723875‑M2/133‑1 (MQ=255)                                                                        cggtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATctactca                                                                                                                                                                                                                                             <  1:825214‑M2/51‑4 (MQ=255)                                                                          gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTAt                                                                                                                                                                                                                                                    >  1:798201‑M2/116‑159 (MQ=255)                                                                          gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCAtt                                                                                                                                                        >  1:11000‑M2/116‑251 (MQ=255)                                                                          gtaactaaccaaccaccggaaccggcaaaaggcgatatatttgaagataacggcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCt                                                                                                                                                                                                                                                  <  1:386557‑M2/46‑1 (MQ=255)                                                                                 accaaccaccggaaccggcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTc                                                                                                                                                 <  1:556672‑M2/143‑1 (MQ=255)                                                                                                  gcaaaaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAg                                                                                                                                >  1:397013‑M2/92‑251 (MQ=255)                                                                                                      aaggcgatatatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAAcc                                                                                                                            <  1:465062‑M2/164‑1 (MQ=255)                                                                                                               tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                   >  1:657763‑M2/79‑251 (MQ=255)                                                                                                               tatttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGAtt                                                                                                                   >  1:534921‑M2/79‑251 (MQ=255)                                                                                                                atttgaagataacgtcagtacgtttaaagcaggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAata                                                                                                                                                    <  1:895880‑M2/140‑1 (MQ=255)                                                                                                                                               ggtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAt                                                                                   >  1:558750‑M2/47‑251 (MQ=255)                                                                                                                                                gtaaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                    <  1:877090‑M2/204‑1 (MQ=255)                                                                                                                                                 taaagcccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGAtt                                                                                  <  1:55230‑M2/206‑1 (MQ=255)                                                                                                                                                      cccataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGc                                                                             <  1:291545‑M2/211‑1 (MQ=255)                                                                                                                                                         ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                         >  1:508186‑M2/37‑251 (MQ=255)                                                                                                                                                         ataaactcccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGAtg                                                                         >  1:482138‑M2/37‑251 (MQ=255)                                                                                                                                                                cccggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGa                                                                  <  1:288050‑M2/222‑1 (MQ=255)                                                                                                                                                                   ggtcaatgccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGaa                                                                 <  1:605604‑M2/223‑1 (MQ=255)                                                                                                                                                                          gccaatttactgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATc                                                                                                                                                                                                                                                   <  1:624423‑M2/45‑1 (MQ=255)                                                                                                                                                                                  actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGc                                                                                                                                        >  1:374985/10‑163 (MQ=255)                                                                                                                                                                                  actgttggcaaTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATagga                                                <  1:248387‑M2/240‑1 (MQ=255)                                                                                                                                                                                            aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGATTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                      <  1:456171/251‑3 (MQ=255)                                                                                                                                                                                            aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                      <  1:514503‑M2/250‑3 (MQ=255)                                                                                                                                                                                            aTGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGgtataaacc                                      <  1:212741‑M2/250‑3 (MQ=255)                                                                                                                                                                                                 atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGa                                                                                    >  1:157540/1‑200 (MQ=255)                                                                                                                                                                                                 atcatGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaaccatgg                                  >  1:505424/1‑244 (MQ=255)                                                                                                                                                                                                    atGTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaacc                                      <  1:196232/243‑3 (MQ=255)                                                                                                                                                                                                      gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                            >  1:103920/1‑251 (MQ=255)                                                                                                                                                                                                      gTTAAGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATaa                            >  1:468242/1‑251 (MQ=255)                                                                                                                                                                                                          aGCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAct                         >  1:221211/1‑250 (MQ=255)                                                                                                                                                                                                           gCATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTataaac                                       <  1:4890/235‑2 (MQ=255)                                                                                                                                                                                                            cATGATCACCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCtt                      >  1:654303/1‑251 (MQ=255)                                                                                                                                                                                                                   aCCGATTCAACCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGa               <  1:711969/251‑1 (MQ=255)                                                                                                                                                                                                                           aaCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCaa       <  1:393779/251‑1 (MQ=255)                                                                                                                                                                                                                            aCCAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAt      <  1:367161/251‑1 (MQ=255)                                                                                                                                                                                                                             ccAGTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtt     <  1:849504/251‑1 (MQ=255)                                                                                                                                                                                                                                gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAAc                          <  1:201633/227‑1 (MQ=255)                                                                                                                                                                                                                                gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca  >  1:379915/1‑249 (MQ=255)                                                                                                                                                                                                                                gTGCATTATCTACTCAATTAGCCAAACGGGGGACATTTAATCCGACTGATACGGTACAAGCCGGCTCTGAAATTGCACAAAGTATCCTGGAGAAAACCATTAATATTCCGCCTACGCTTTATAAGAACCAGGGGGATTTAGTAGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTATACGAATTGAAACAGAAATAGGAGGTATAAACCATGGCCATAAACTCTTCCGGCGATGTATCAAtttca  >  1:271678/1‑249 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      TCTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGTACACCGAAAAATATCATCATCAACATTAATTCCGGCGCAACCGATGAATTAGGCGGAACCGGTATTCCGGGCTATGTAGATAATCATTTCTGGGAACGTTTCGGTAATGCCATCATGTTAAGCATGATCACCGATTCAACCAGTGCACTATCTACCCAATTAGCAAAACGCGGAACTTTCCAACCAACGGATACGGTACAAGCCGGCTCGGAAATTGCACAAAGTATCTTAGAGAAAACCATTAATATTCCCCCTACGCTTTATAAGAACCAAGGGGATTTAGTGGGGATTTTTGTTGCGCGGGATATTGATTTTGGCGATGTGTACGAATTGAAACAGAAATAGGAGGTATAAATAATGGTCATAAACTCTTCCGGCGATGTATCAATTTTA  >  1/1202488‑1202961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑