Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,325	G→C	intergenic (+42745/+218612)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,325	0	G	C	100.0%	209.8 / NA	73	intergenic (+42745/+218612)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (41/32);  total (41/32)

ACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGCGGGTAAAGCGCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAAAGGCACTTTTATTCAGTGTTCCTTACGCACAAAACTTGTTTCCACCGTGGCCGGCAATCTCGGCTGCGTTGTGGCAAACGATGTATATAGTGCAAACGGCACGGTACTACTGATTGAAAAAGGCTCTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGT  >  1/1202082‑1202568                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ccaACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                                                                                              <  1:565268/250‑1 (MQ=255) acaACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                                                                                              <  1:726828/251‑1 (MQ=255)          gccTACCGAAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCTTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                                                                                              <  1:46284/241‑1 (MQ=255)              aCCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATg                                                                                                                                                                                                                                  >  1:875313/1‑250 (MQ=255)                        cTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTa                                                                                                                                                                                                                          >  1:850764/1‑248 (MQ=255)                             gTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGTAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTgg                                                                                                                                                                                                                   >  1:93970/1‑250 (MQ=255)                                 aaaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAt                                                                                                                                                                                                               <  1:436239‑M1/250‑2 (MQ=255)                                  aaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtg                                                                                                                                                                                                              >  1:572534‑M1/1‑248 (MQ=255)                                    aTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTACCGATGCCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATCCAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGCCAGTAGGTTTAAAGCAGGTAAAGCCCATAAACCCCCGGTCAATTCCACTTTACTGTTGGCattgcca                                                                                                                                                                                                           >  1:711814/1‑245 (MQ=255)                                     tCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAAttt                                                                                                                                                                                                                           >  1:60336/1‑234 (MQ=255)                                           aacGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgt                                                                                                                                                                                                     <  1:824851‑M1/249‑12 (MQ=255)                                               aCAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATACCGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgt                                                                                                                                                                                                     >  1:438377‑M1/1‑235 (MQ=255)                                                   agGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagca                                                                                                                                                                                               <  1:735888‑M1/248‑18 (MQ=255)                                                    ggCGTTAGATATGCCATCCGCCCCACGTTTAATCAATGGATTGTCTCCGGGTACTATCGATGGCGAACCGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCGGTCCGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTAc                                                                                                                                                                                                                         >  1:427208/1‑221 (MQ=255)                                                        ttAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcac                                                                                                                                                                                        <  1:53515‑M1/250‑25 (MQ=255)                                                        ttAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagca                                                                                                                                                                                               >  1:340545‑M1/1‑226 (MQ=255)                                                           gATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgat                                                                                                                                                                                    >  1:762157‑M1/1‑223 (MQ=255)                                                           gATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgat                                                                                                                                                                                    >  1:465065‑M1/1‑223 (MQ=255)                                                                       gCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgca                                                                                                                                                                        >  1:421122‑M1/1‑211 (MQ=255)                                                                             cGTTTAATCAAGGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                                                                                                <  1:851247‑M1/221‑17 (MQ=255)                                                                             cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTg                                                                                                                                                                                                                       >  1:118425/1‑198 (MQ=255)                                                                             cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaacc                                                                                                                                                                              >  1:221399‑M1/1‑205 (MQ=255)                                                                             cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                                                                                                >  1:226926‑M1/1‑205 (MQ=255)                                                                                              gTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatc                                                                                                                                                                   >  1:317537‑M1/1‑188 (MQ=255)                                                                                               tctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgg                                                                                                                                                <  1:31311‑M1/251‑65 (MQ=255)                                                                                                ctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacggg                                                                                                                                               >  1:443178‑M1/1‑186 (MQ=255)                                                                                                ctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatg                                                                                                                                                                                                      >  1:119367‑M1/1‑186 (MQ=255)                                                                                                      gTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatct                                                                                                                                                                  <  1:95516‑M1/226‑47 (MQ=255)                                                                                                      gTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACATTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacat                                                                                                                                         >  1:204564‑M1/1‑180 (MQ=255)                                                                                                        aCTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacattt                                                                                                                                       <  1:312825‑M1/251‑74 (MQ=255)                                                                                                        aCTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattat                                                                                                                                                                    >  1:628103‑M1/1‑178 (MQ=255)                                                                                                           aTCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaat                                                                                                                                    >  1:461564‑M1/1‑175 (MQ=255)                                                                                                                                 ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                                                               <  1:846658‑M1/250‑98 (MQ=255)                                                                                                                                 ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                                                               <  1:669835‑M1/250‑98 (MQ=255)                                                                                                                                 ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagc                                                                                                                <  1:14698‑M1/249‑97 (MQ=255)                                                                                                                                   gTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                                                               >  1:199476‑M1/1‑151 (MQ=255)                                                                                                                                    tttCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccgg                                                                                                             >  1:564717‑M1/1‑150 (MQ=255)                                                                                                                                         gatGTTCAGAATACAGATACAACTGACACGGTAACTAACCTACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                                                                                                <  1:84462‑M1/160‑17 (MQ=255)                                                                                                                                         gatGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaa                                                                                                      >  1:233769‑M1/2‑145 (MQ=255)                                                                                                                                          aTGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaa                                                                                                     <  1:861862‑M1/251‑108 (MQ=255)                                                                                                                                             ttCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                                                                                                  >  1:707011‑M1/1‑141 (MQ=255)                                                                                                                                             ttCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                                                                                                  >  1:257774‑M1/1‑141 (MQ=255)                                                                                                                                              tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgc                                                                                                 <  1:659247‑M1/251‑112 (MQ=255)                                                                                                                                              tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                                                                                                  >  1:223166‑M1/1‑140 (MQ=255)                                                                                                                                                      cAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaatt                                                                                                                                                          <  1:755700‑M1/186‑55 (MQ=255)                                                                                                                                                       aGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                                                                                        >  1:389129‑M1/1‑131 (MQ=255)                                                                                                                                                        gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                                                                                        >  1:344739‑M1/1‑130 (MQ=255)                                                                                                                                                        gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                                                                                        >  1:119187‑M1/1‑130 (MQ=255)                                                                                                                                                        gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagcc                                                                                                                                                      <  1:611436‑M1/188‑59 (MQ=255)                                                                                                                                                         aTACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcc                                                                                      <  1:702748‑M1/251‑123 (MQ=255)                                                                                                                                                         aTACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagt                                                                                          <  1:312015‑M1/247‑119 (MQ=255)                                                                                                                                                            cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctgg                                                                                   <  1:445681‑M1/251‑126 (MQ=255)                                                                                                                                                            cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                                                               >  1:205200‑M1/1‑126 (MQ=255)                                                                                                                                                            cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgtta                                                                                                                                                                                                   <  1:80894‑M1/139‑14 (MQ=255)                                                                                                                                                              aCTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggag                                                                                 <  1:649342‑M1/251‑128 (MQ=255)                                                                                                                                                              aCTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacggggg                                                                                                                                             >  1:299954‑M1/1‑124 (MQ=255)                                                                                                                                                               cTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggaga                                                                                >  1:100574‑M1/1‑123 (MQ=255)                                                                                                                                                                    acGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaacc                                                                           <  1:723875‑M1/251‑134 (MQ=255)                                                                                                                                                                     cGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactca                                                                                                                                                             <  1:825214‑M1/168‑52 (MQ=255)                                                                                                                                                                       gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccatt                                                                        >  1:11000‑M1/1‑115 (MQ=255)                                                                                                                                                                       gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattat                                                                                                                                                                    >  1:798201‑M1/1‑115 (MQ=255)                                                                                                                                                                       gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatg                                                                                                                                                                                                      >  1:505888‑M1/1‑115 (MQ=255)                                                                                                                                                                       gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGGCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatct                                                                                                                                                                  <  1:386557‑M1/161‑47 (MQ=255)                                                                                                                                                                              accaaCCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattc                                                                 <  1:556672‑M1/249‑144 (MQ=255)                                                                                                                                                                                               gCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataag                                                >  1:397013‑M1/1‑91 (MQ=255)                                                                                                                                                                                                   aaGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaacc                                            <  1:465062‑M1/251‑165 (MQ=255)                                                                                                                                                                                                    aGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGTTCAATGCCAATTTACTGTTGGCAAtgccatca                                                                                                                                                                                                        >  1:45897‑M1/1‑86 (MQ=255)                                                                                                                                                                                                            tatttgaAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaaccagggggatt                                   >  1:657763‑M1/3‑78 (MQ=255)                                                                                                                                                                                                            tatttgaAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaaccagggggatt                                   >  1:534921‑M1/3‑78 (MQ=255)                                                                                                                                                                                                             atttgAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaata                                                                    <  1:895880‑M1/216‑141 (MQ=255)                                                                                                                                                                                                                                            ggTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaaccagggggatttagtagggatttttgttgcgcgggatattgat   >  1:558750‑M1/1‑46 (MQ=255)                                                                                                                                                                                                                                             gTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaaccagggggatttagtagggatttttgttgcgcgggatattga    <  1:877090‑M1/249‑205 (MQ=255)                                                                                                                                                                                                                                              tAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaaccagggggatttagtagggatttttgttgcgcgggatattgatt  <  1:55230‑M1/250‑207 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGCGGGTAAAGCGCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAAAGGCACTTTTATTCAGTGTTCCTTACGCACAAAACTTGTTTCCACCGTGGCCGGCAATCTCGGCTGCGTTGTGGCAAACGATGTATATAGTGCAAACGGCACGGTACTACTGATTGAAAAAGGCTCTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAGCAAGGCGAAGAACGTTTATTTGTAGTCTGGAGTGAAATCCGT  >  1/1202082‑1202568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑