Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,286	G→A	intergenic (+42706/+218651)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,286	0	G	A	100.0%	201.1 / NA	75	intergenic (+42706/+218651)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/34);  total (41/34)

ACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAGCCACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGCGGGTAAAGCGCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAAAGGCACTTTTATTCAGTGTTCCTTACGCACAAAACTTGTTTCCACCGTGGCCGGCAATCTCGGCTGCGTTGTGGCAAACGATGTATATAGTGCAAACGGCACGGTACTACTGATTGAAAAAGGCTCTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAG  >  1/1202043‑1202526                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 cccaccGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAg                                                                                                                                                                                                                                           <  1:186330/250‑1 (MQ=255)                       aTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc                                                                                                                                                                                                                     <  1:399249/251‑1 (MQ=255)                       aTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc                                                                                                                                                                                                                     <  1:762/251‑1 (MQ=255)                                        ccaACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                                                    <  1:565268/250‑1 (MQ=255)                                        acaACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                                                    <  1:726828/251‑1 (MQ=255)                                         caACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc                                                                                                                                                                                                                     >  1:642938/1‑233 (MQ=255)                                                 gccTACCGAAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCTTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                                                    <  1:46284/241‑1 (MQ=255)                                                    tACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGAt                                                                                                                                                                                                                                         >  1:826641/1‑202 (MQ=255)                                                     aCCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATg                                                                                                                                                                                        >  1:875313/1‑250 (MQ=255)                                                               cTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTa                                                                                                                                                                                >  1:850764/1‑248 (MQ=255)                                                                    gTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGTAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTgg                                                                                                                                                                         >  1:93970/1‑250 (MQ=255)                                                                        aaaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAt                                                                                                                                                                     <  1:436239‑M1/250‑2 (MQ=255)                                                                         aaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAg                                                                                                                                                                                                                                           <  1:398173/179‑1 (MQ=255)                                                                         aaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtg                                                                                                                                                                    >  1:572534‑M1/1‑248 (MQ=255)                                                                           aTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTACCGATGCCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATCCAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGCCAGTAGGTTTAAAGCAGGTAAAGCCCATAAACCCCCGGTCAATTCCACTTTACTGTTGGCattgcca                                                                                                                                                                 >  1:711814/1‑245 (MQ=255)                                                                            tCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAAttt                                                                                                                                                                                 >  1:60336/1‑234 (MQ=255)                                                                                  aacGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgt                                                                                                                                                           <  1:824851‑M1/249‑12 (MQ=255)                                                                                      aCAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATACCGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgt                                                                                                                                                           >  1:438377‑M1/1‑235 (MQ=255)                                                                                          agGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagca                                                                                                                                                     <  1:735888‑M1/248‑18 (MQ=255)                                                                                           ggCGTTAGATATGCCATCCGCCCCACGTTTAATCAATGGATTGTCTCCGGGTACTATCGATGGCGAACCGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCGGTCCGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTAc                                                                                                                                                                               >  1:427208/1‑221 (MQ=255)                                                                                               ttAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcac                                                                                                                                              <  1:53515‑M1/250‑25 (MQ=255)                                                                                               ttAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagca                                                                                                                                                     >  1:340545‑M1/1‑226 (MQ=255)                                                                                                  gATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgat                                                                                                                                          >  1:465065‑M1/1‑223 (MQ=255)                                                                                                  gATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgat                                                                                                                                          >  1:762157‑M1/1‑223 (MQ=255)                                                                                                              gCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgca                                                                                                                              >  1:421122‑M1/1‑211 (MQ=255)                                                                                                                    cGTTTAATCAAGGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                                                      <  1:851247‑M1/221‑17 (MQ=255)                                                                                                                    cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTg                                                                                                                                                                             >  1:118425/1‑198 (MQ=255)                                                                                                                    cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaacc                                                                                                                                    >  1:221399‑M1/1‑205 (MQ=255)                                                                                                                    cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                                                      >  1:226926‑M1/1‑205 (MQ=255)                                                                                                                                     gTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatc                                                                                                                         >  1:317537‑M1/1‑188 (MQ=255)                                                                                                                                      tctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgg                                                                                                      <  1:31311‑M1/251‑65 (MQ=255)                                                                                                                                       ctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacggg                                                                                                     >  1:443178‑M1/1‑186 (MQ=255)                                                                                                                                       ctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatg                                                                                                                                                            >  1:119367‑M1/1‑186 (MQ=255)                                                                                                                                           gggTACTATCGAGGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTg                                                                                                                                                                                                                                              <  1:552084/110‑1 (MQ=255)                                                                                                                                            ggTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAgc                                                                                                                                                                                                            >  1:833901/1‑143 (MQ=255)                                                                                                                                             gTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatct                                                                                                                        <  1:95516‑M1/226‑47 (MQ=255)                                                                                                                                             gTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACATTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacat                                                                                               >  1:204564‑M1/1‑180 (MQ=255)                                                                                                                                               aCTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacattt                                                                                             <  1:312825‑M1/251‑74 (MQ=255)                                                                                                                                               aCTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattat                                                                                                                          >  1:628103‑M1/1‑178 (MQ=255)                                                                                                                                                  aTCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaat                                                                                          >  1:461564‑M1/1‑175 (MQ=255)                                                                                                                                                                        ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                     <  1:846658‑M1/250‑98 (MQ=255)                                                                                                                                                                        ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                     <  1:669835‑M1/250‑98 (MQ=255)                                                                                                                                                                        ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagc                                                                      <  1:14698‑M1/249‑97 (MQ=255)                                                                                                                                                                          gTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                     >  1:199476‑M1/1‑151 (MQ=255)                                                                                                                                                                           tttCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccgg                                                                   >  1:564717‑M1/1‑150 (MQ=255)                                                                                                                                                                                gatGTTCAGAATACAGATACAACTGACACGGTAACTAACCTACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                                                      <  1:84462‑M1/160‑17 (MQ=255)                                                                                                                                                                                gatGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaa                                                            >  1:233769‑M1/2‑145 (MQ=255)                                                                                                                                                                                 aTGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaa                                                           <  1:861862‑M1/251‑108 (MQ=255)                                                                                                                                                                                    ttCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                                                        >  1:257774‑M1/1‑141 (MQ=255)                                                                                                                                                                                    ttCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                                                        >  1:707011‑M1/1‑141 (MQ=255)                                                                                                                                                                                     tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgc                                                       <  1:659247‑M1/251‑112 (MQ=255)                                                                                                                                                                                     tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                                                        >  1:223166‑M1/1‑140 (MQ=255)                                                                                                                                                                                             cAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaatt                                                                                                                <  1:755700‑M1/186‑55 (MQ=255)                                                                                                                                                                                              aGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                                              >  1:389129‑M1/1‑131 (MQ=255)                                                                                                                                                                                               gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                                              >  1:344739‑M1/1‑130 (MQ=255)                                                                                                                                                                                               gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                                              >  1:119187‑M1/1‑130 (MQ=255)                                                                                                                                                                                               gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagcc                                                                                                            <  1:611436‑M1/188‑59 (MQ=255)                                                                                                                                                                                                aTACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcc                                            <  1:702748‑M1/251‑123 (MQ=255)                                                                                                                                                                                                aTACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagt                                                <  1:312015‑M1/247‑119 (MQ=255)                                                                                                                                                                                                   cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctgg                                         <  1:445681‑M1/251‑126 (MQ=255)                                                                                                                                                                                                   cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                                                     >  1:205200‑M1/1‑126 (MQ=255)                                                                                                                                                                                                   cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgtta                                                                                                                                                         <  1:80894‑M1/139‑14 (MQ=255)                                                                                                                                                                                                     aCTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggag                                       <  1:649342‑M1/251‑128 (MQ=255)                                                                                                                                                                                                     aCTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacggggg                                                                                                   >  1:299954‑M1/1‑124 (MQ=255)                                                                                                                                                                                                      cTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggaga                                      >  1:100574‑M1/1‑123 (MQ=255)                                                                                                                                                                                                           acGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaacc                                 <  1:723875‑M1/251‑134 (MQ=255)                                                                                                                                                                                                            cGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactca                                                                                                                   <  1:825214‑M1/168‑52 (MQ=255)                                                                                                                                                                                                              gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccatt                              >  1:11000‑M1/1‑115 (MQ=255)                                                                                                                                                                                                              gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattat                                                                                                                          >  1:798201‑M1/1‑115 (MQ=255)                                                                                                                                                                                                              gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatg                                                                                                                                                            >  1:505888‑M1/1‑115 (MQ=255)                                                                                                                                                                                                              gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGGCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatct                                                                                                                        <  1:386557‑M1/161‑47 (MQ=255)                                                                                                                                                                                                                     accaaCCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattc                       <  1:556672‑M1/249‑144 (MQ=255)                                                                                                                                                                                                                                      gCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataag      >  1:397013‑M1/1‑91 (MQ=255)                                                                                                                                                                                                                                          aaGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccattaatattccgcctacgctttataagaacc  <  1:465062‑M1/251‑165 (MQ=255)                                                                                                                                                                                                                                           aGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGTTCAATGCCAATTTACTGTTGGCAAtgccatca                                                                                                                                                              >  1:45897‑M1/1‑86 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAGCCACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGCGGGTAAAGCGCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAAAGGCACTTTTATTCAGTGTTCCTTACGCACAAAACTTGTTTCCACCGTGGCCGGCAATCTCGGCTGCGTTGTGGCAAACGATGTATATAGTGCAAACGGCACGGTACTACTGATTGAAAAAGGCTCTACCGTGTTCGGTGAGTTCCGTAGCGGTCAGATTCAG  >  1/1202043‑1202526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑