Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,256	T→C	intergenic (+42676/+218681)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,256	0	T	C	100.0%	218.0 / NA	78	intergenic (+42676/+218681)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/34);  total (44/34)

CGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAGCCACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGCGGGTAAAGCGCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAAAGGCACTTTTATTCAGTGTTCCTTACGCACAAAACTTGTTTCCACCGTGGCCGGCAATCTCGGCTGCGTTGTGGCAAACGATGTATATAGTGCAAACGGCACGGTACTACTGATTGAAAAAGGCTCTACCGTGTT  >  1/1202010‑1202498                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCaa                                                                                                                                                                                                                                                 >  1:892278/1‑250 (MQ=255)   gTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAaccaacca                                                                                                                                                                                                                                              >  1:50322/1‑251 (MQ=255)     acgacgGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAaccac                                                                                                                                                                                                                                             >  1:395665/1‑250 (MQ=255)                ttCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCaa                                                                                                                                                                                                                                 >  1:848378/1‑251 (MQ=255)                        cATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGat                                                                                                                                                                                                                         >  1:484210/1‑251 (MQ=255)                           ttACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGatata                                                                                                                                                                                                                      <  1:264328/251‑2 (MQ=255)                                  cccaccGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAg                                                                                                                                                                                                               <  1:186330/250‑1 (MQ=255)                                                        aTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc                                                                                                                                                                                         <  1:762/251‑1 (MQ=255)                                                        aTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc                                                                                                                                                                                         <  1:399249/251‑1 (MQ=255)                                                                         ccaACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                        <  1:565268/250‑1 (MQ=255)                                                                         acaACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                        <  1:726828/251‑1 (MQ=255)                                                                          caACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc                                                                                                                                                                                         >  1:642938/1‑233 (MQ=255)                                                                            aCCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGatata                                                                                                                                                                                                                      <  1:593475/202‑2 (MQ=255)                                                                                  gccTACCGAAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCTTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAAc                                                                                                                                                                        <  1:46284/241‑1 (MQ=255)                                                                                     tACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGAt                                                                                                                                                                                                             >  1:826641/1‑202 (MQ=255)                                                                                      aCCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATg                                                                                                                                                            >  1:875313/1‑250 (MQ=255)                                                                                                cTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTa                                                                                                                                                    >  1:850764/1‑248 (MQ=255)                                                                                                     gTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGTAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTgg                                                                                                                                             >  1:93970/1‑250 (MQ=255)                                                                                                         aaaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAt                                                                                                                                         <  1:436239‑M1/250‑2 (MQ=255)                                                                                                          aaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAg                                                                                                                                                                                                               <  1:398173/179‑1 (MQ=255)                                                                                                          aaaTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtg                                                                                                                                        >  1:572534‑M1/1‑248 (MQ=255)                                                                                                            aTCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTACCGATGCCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATCCAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGCCAGTAGGTTTAAAGCAGGTAAAGCCCATAAACCCCCGGTCAATTCCACTTTACTGTTGGCattgcca                                                                                                                                     >  1:711814/1‑245 (MQ=255)                                                                                                             tCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAAttt                                                                                                                                                     >  1:60336/1‑234 (MQ=255)                                                                                                                   aacGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgt                                                                                                                               <  1:824851‑M1/249‑12 (MQ=255)                                                                                                                       aCAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATACCGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgt                                                                                                                               >  1:438377‑M1/1‑235 (MQ=255)                                                                                                                           agGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagca                                                                                                                         <  1:735888‑M1/248‑18 (MQ=255)                                                                                                                            ggCGTTAGATATGCCATCCGCCCCACGTTTAATCAATGGATTGTCTCCGGGTACTATCGATGGCGAACCGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCGGTCCGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTAc                                                                                                                                                   >  1:427208/1‑221 (MQ=255)                                                                                                                                ttAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcac                                                                                                                  <  1:53515‑M1/250‑25 (MQ=255)                                                                                                                                ttAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagca                                                                                                                         >  1:340545‑M1/1‑226 (MQ=255)                                                                                                                                   gATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgat                                                                                                              >  1:762157‑M1/1‑223 (MQ=255)                                                                                                                                   gATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgat                                                                                                              >  1:465065‑M1/1‑223 (MQ=255)                                                                                                                                               gCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgca                                                                                                  >  1:421122‑M1/1‑211 (MQ=255)                                                                                                                                                     cGTTTAATCAAGGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                          <  1:851247‑M1/221‑17 (MQ=255)                                                                                                                                                     cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTg                                                                                                                                                 >  1:118425/1‑198 (MQ=255)                                                                                                                                                     cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaacc                                                                                                        >  1:221399‑M1/1‑205 (MQ=255)                                                                                                                                                     cGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                          >  1:226926‑M1/1‑205 (MQ=255)                                                                                                                                                                      gTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatc                                                                                             >  1:317537‑M1/1‑188 (MQ=255)                                                                                                                                                                       tctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgg                                                                          <  1:31311‑M1/251‑65 (MQ=255)                                                                                                                                                                        ctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacggg                                                                         >  1:443178‑M1/1‑186 (MQ=255)                                                                                                                                                                        ctcCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatg                                                                                                                                >  1:119367‑M1/1‑186 (MQ=255)                                                                                                                                                                            gggTACTATCGAGGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTg                                                                                                                                                                                                                  <  1:552084/110‑1 (MQ=255)                                                                                                                                                                             ggTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAgc                                                                                                                                                                                >  1:833901/1‑143 (MQ=255)                                                                                                                                                                              gTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatct                                                                                            <  1:95516‑M1/226‑47 (MQ=255)                                                                                                                                                                              gTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACATTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacat                                                                   >  1:204564‑M1/1‑180 (MQ=255)                                                                                                                                                                                aCTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacattt                                                                 <  1:312825‑M1/251‑74 (MQ=255)                                                                                                                                                                                aCTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattat                                                                                              >  1:628103‑M1/1‑178 (MQ=255)                                                                                                                                                                                   aTCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaat                                                              >  1:461564‑M1/1‑175 (MQ=255)                                                                                                                                                                                                         ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                         <  1:846658‑M1/250‑98 (MQ=255)                                                                                                                                                                                                         ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                         <  1:669835‑M1/250‑98 (MQ=255)                                                                                                                                                                                                         ccGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagc                                          <  1:14698‑M1/249‑97 (MQ=255)                                                                                                                                                                                                           gTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                         >  1:199476‑M1/1‑151 (MQ=255)                                                                                                                                                                                                            tttCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccgg                                       >  1:564717‑M1/1‑150 (MQ=255)                                                                                                                                                                                                                 gatGTTCAGAATACAGATACAACTGACACGGTAACTAACCTACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagc                                                                                                                          <  1:84462‑M1/160‑17 (MQ=255)                                                                                                                                                                                                                 gatGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaa                                >  1:233769‑M1/2‑145 (MQ=255)                                                                                                                                                                                                                  aTGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaa                               <  1:861862‑M1/251‑108 (MQ=255)                                                                                                                                                                                                                     ttCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                            >  1:707011‑M1/1‑141 (MQ=255)                                                                                                                                                                                                                     ttCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                            >  1:257774‑M1/1‑141 (MQ=255)                                                                                                                                                                                                                      tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAgg                                                                                                                                                                                                                             >  1:500543/1‑57 (MQ=255)                                                                                                                                                                                                                      tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgc                           <  1:659247‑M1/251‑112 (MQ=255)                                                                                                                                                                                                                      tCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattg                            >  1:223166‑M1/1‑140 (MQ=255)                                                                                                                                                                                                                              cAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaatt                                                                                    <  1:755700‑M1/186‑55 (MQ=255)                                                                                                                                                                                                                               aGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                  >  1:389129‑M1/1‑131 (MQ=255)                                                                                                                                                                                                                                gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                  >  1:344739‑M1/1‑130 (MQ=255)                                                                                                                                                                                                                                gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtat                  >  1:119187‑M1/1‑130 (MQ=255)                                                                                                                                                                                                                                gATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagcc                                                                                <  1:611436‑M1/188‑59 (MQ=255)                                                                                                                                                                                                                                 aTACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcc                <  1:702748‑M1/251‑123 (MQ=255)                                                                                                                                                                                                                                 aTACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagt                    <  1:312015‑M1/247‑119 (MQ=255)                                                                                                                                                                                                                                    cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctgg             <  1:445681‑M1/251‑126 (MQ=255)                                                                                                                                                                                                                                    cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagcc                                         >  1:205200‑M1/1‑126 (MQ=255)                                                                                                                                                                                                                                    cAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgtta                                                                                                                             <  1:80894‑M1/139‑14 (MQ=255)                                                                                                                                                                                                                                      aCTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggag           <  1:649342‑M1/251‑128 (MQ=255)                                                                                                                                                                                                                                      aCTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacggggg                                                                       >  1:299954‑M1/1‑124 (MQ=255)                                                                                                                                                                                                                                       cTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggaga          >  1:100574‑M1/1‑123 (MQ=255)                                                                                                                                                                                                                                            acGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaacc     <  1:723875‑M1/251‑134 (MQ=255)                                                                                                                                                                                                                                             cGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactca                                                                                       <  1:825214‑M1/168‑52 (MQ=255)                                                                                                                                                                                                                                               gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatctactcaattagccaaacgggggacatttaatccgactgatacggtacaagccggctctgaaattgcacaaagtatcctggagaaaaccatt  >  1:11000‑M1/1‑115 (MQ=255)                                                                                                                                                                                                                                               gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattat                                                                                              >  1:798201‑M1/1‑115 (MQ=255)                                                                                                                                                                                                                                               gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatg                                                                                                                                >  1:505888‑M1/1‑115 (MQ=255)                                                                                                                                                                                                                                               gTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGGCAGTACGTTTAAAGCAGGTAAAGCCCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAtgccatcatgttaagcatgatcaccgattcaaccagtgcattatct                                                                                            <  1:386557‑M1/161‑47 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAGCCACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGCGGGTAAAGCGCATAAACTCCCGGTCAATGCCAATTTACTGTTGGCAAAAGGCACTTTTATTCAGTGTTCCTTACGCACAAAACTTGTTTCCACCGTGGCCGGCAATCTCGGCTGCGTTGTGGCAAACGATGTATATAGTGCAAACGGCACGGTACTACTGATTGAAAAAGGCTCTACCGTGTT  >  1/1202010‑1202498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑