Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,079	G→A	intergenic (+42499/+218858)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,079	0	G	A	100.0%	199.6 / NA	72	intergenic (+42499/+218858)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/36);  total (36/36)

TGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAGCCACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGC  >  1/1201836‑1202315                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             tGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAAc                                                                                                                                                                                                                                       <  1:155866/251‑1 (MQ=255)         cGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCt                                                                                                                                                                                                                               <  1:148901/251‑1 (MQ=255)                                             ccAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGAc                                                                                                                                                                                            <  1:822487/250‑1 (MQ=255)                                                    cgGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCg                                                                                                                                                                                     <  1:717041/250‑1 (MQ=255)                                                         aaaaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGat                                                                                                                                                                               <  1:830719/251‑1 (MQ=255)                                                         aaaaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGat                                                                                                                                                                               <  1:648330/251‑1 (MQ=255)                                                           aaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGatat                                                                                                                                                                             >  1:178901/1‑251 (MQ=255)                                                           aaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGa                                                                                                                                                                                >  1:887704/1‑248 (MQ=255)                                                              cAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGatatgcc                                                                                                                                                                          <  1:61490/251‑4 (MQ=255)                                                                  aTCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATc                                                                                                                                                                       >  1:853096/1‑250 (MQ=255)                                                                  aTCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATc                                                                                                                                                                       >  1:188800/1‑250 (MQ=255)                                                                   tCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCg                                                                                                                                                                     >  1:53165/1‑251 (MQ=255)                                                                            ttttCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGtt                                                                                                                                                            <  1:571736/251‑1 (MQ=255)                                                                                  gATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCa                                                                                                                                                      >  1:664779/1‑251 (MQ=255)                                                                                    ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGat                                                                                                                                                                               >  1:518547/1‑224 (MQ=255)                                                                                    ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCa                                                                                                                                                                         <  1:815096/230‑1 (MQ=255)                                                                                    ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGccc                                                                                                                                                                  <  1:660980/237‑1 (MQ=255)                                                                                    ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCaaa                                                                                                                                                    >  1:763379/1‑251 (MQ=255)                                                                                      attGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAgg                                                                                                                                                  <  1:812013/251‑1 (MQ=255)                                                                                       ttGCTCTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAgg                                                                                                                                                                                       <  1:147723/213‑1 (MQ=255)                                                                                       ttGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAg                                                                                                                                                                                                            <  1:241651/192‑1 (MQ=255)                                                                                            cTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGt                                                                                                                                             >  1:51334/1‑250 (MQ=255)                                                                                              gCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGCTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGtct                                                                                                                                           >  1:737058/1‑250 (MQ=255)                                                                                                       ggCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATc                                                                                                                                                       <  1:579407/229‑1 (MQ=255)                                                                                                          gTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCa                                                                                                                                                                         >  1:774243/1‑208 (MQ=255)                                                                                                           tttttGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCg                                                                                                                              <  1:845213/250‑1 (MQ=255)                                                                                                                gggTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAgg                                                                                                                                                                                       <  1:159999/188‑1 (MQ=255)                                                                                                                gggTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGa                                                                                                                                                                                >  1:721287/1‑195 (MQ=255)                                                                                                                gggTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCg                                                                                                                        >  1:204104/1‑251 (MQ=255)                                                                                                                     aTTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGatatgcc                                                                                                                                                                          >  1:878165/1‑193 (MQ=255)                                                                                                                       tCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCACAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGtt                                                                                                                 >  1:415863/1‑251 (MQ=255)                                                                                                                             ttGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGc                                                                                                           <  1:161281/251‑1 (MQ=255)                                                                                                                                      aaGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGtct                                                                                                                                           <  1:487867/210‑1 (MQ=255)                                                                                                                                        ggATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGccccacg                                                                                                                                                              >  1:715761/1‑186 (MQ=255)                                                                                                                                         gATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATg                                                                                                <  1:770005/250‑1 (MQ=255)                                                                                                                                           tACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTc                                                                                             <  1:477622/251‑1 (MQ=255)                                                                                                                                               cAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTc                                                                                             >  1:106446/1‑247 (MQ=255)                                                                                                                                                aaCCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAAt                                                                                        <  1:494765/251‑1 (MQ=255)                                                                                                                                                    ggCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTAc                                                                                                                                   >  1:868652/1‑204 (MQ=255)                                                                                                                                                     gCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCGATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTAt                                                                                                                                <  1:791342/206‑1 (MQ=255)                                                                                                                                                     gCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAgg                                                                                                                                                                                       >  1:811604/1‑151 (MQ=255)                                                                                                                                                      cacaGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGa                                                                                   <  1:467966/250‑1 (MQ=255)                                                                                                                                                      cacaGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGa                                                                                   <  1:88770/250‑1 (MQ=255)                                                                                                                                                         aGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTAc                                                                                                               >  1:660966/1‑219 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCTGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGt                                                                                                                                                             >  1:232463/1‑172 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTcc                                                                                                                                                                                                                 >  1:114553/1‑120 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGt                                                                                                                                                             >  1:481830/1‑172 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCggg                                                                                                                                      >  1:66401/1‑195 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGa                                                                                   <  1:829485/246‑1 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                                              >  1:537536/1‑251 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                                              >  1:712206/1‑251 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                                              >  1:519502/1‑251 (MQ=255)                                                                                                                                                          ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                                              >  1:366638/1‑251 (MQ=255)                                                                                                                                                            ttAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAAc                                                                             <  1:48056/250‑1 (MQ=255)                                                                                                                                                                 gAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGc                                                                                                           >  1:46622/1‑215 (MQ=255)                                                                                                                                                                 gAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGaca                                                                        <  1:117191/250‑1 (MQ=255)                                                                                                                                                                           aatgcGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGAAGAAATCACGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAAGCATAGAACGACAGAGGCGTTAGATATGCCAACCGCCCCACGTTTAATCAAAGGATTGTATCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTaac                                                             <  1:592228/247‑2 (MQ=255)                                                                                                                                                                           aaTTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTaac                                                             <  1:132038/251‑2 (MQ=255)                                                                                                                                                                            aTTCGGTACGACGGTGCGTTCAAAAACATTTACGGCCCCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTaac                                                             <  1:537108/250‑2 (MQ=255)                                                                                                                                                                               cGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTCCCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATg                                                                                                <  1:217931/212‑1 (MQ=255)                                                                                                                                                                               cGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCaa                                                          >  1:892278/1‑250 (MQ=255)                                                                                                                                                                                 gTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAaccaacca                                                       >  1:50322/1‑251 (MQ=255)                                                                                                                                                                                   acgacgGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAaccac                                                      >  1:395665/1‑250 (MQ=255)                                                                                                                                                                                           gCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAgaa                                                                                                                                                                                                >  1:676221/1‑102 (MQ=255)                                                                                                                                                                                              ttCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCaa                                          >  1:848378/1‑251 (MQ=255)                                                                                                                                                                                               ttcaaaaCATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATa                                                                                 <  1:605665/208‑1 (MQ=255)                                                                                                                                                                                                      cATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGcc                                                                                                          >  1:529468/1‑179 (MQ=255)                                                                                                                                                                                                      cATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGat                                  >  1:484210/1‑251 (MQ=255)                                                                                                                                                                                                         ttACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGatata                               <  1:264328/251‑2 (MQ=255)                                                                                                                                                                                                                cccaccGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAg                        <  1:186330/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc  <  1:399249/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATATTTGAAGATAACGTCAGTACGTTTAAAGc  <  1:762/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             TGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAGCCACAACCAATTCCTACCGCAACCGCTCCAGTAGAAAATCATAGTACGACAGAGGCGTTAGATATTCCATCCGCCCCCCGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAAATGTTCAGAATACAGATACAACTGACACGGTAACTAATCAACCACCGGAACCGGCAAAAGGCGATATGTTTGAAGATAACGTCAGTACGTTTAAAGC  >  1/1201836‑1202315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑