Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,201,758	G→A	intergenic (+42178/+219179)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,201,758	0	G	A	100.0%	160.6 / NA	60	intergenic (+42178/+219179)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/28);  total (32/28)

TTATGATGACGGTATTTTTACTTATCTTGGATTTGCCCCAAATAAAACCTTTCCTGCAGCCTTCTTGTTAGAAGGCTCCACGGAAAGTCTGTTAAATACTAATGTTAAACAAGACGGTAATTATCAGGTATTAGTCATCCAAAAAACAGCAGAAAAATTGGTATTACGTAGCGGTGAAAAAGTGGTCGGCATTTACAATCAAAGTTACGGAAAAACACCGGTAACTTATAACACGACCATTTCACCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAA  >  1/1201524‑1201999                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  tttgccagctcactttttacaattattggcatgacactgacaatttactttttaggaaaaggatttgccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAAtg                                                                                                                                                                                                                                   <  1:710751‑M2/40‑1 (MQ=255)                      attattggcatgacactgacaatttactttttaggaaaaggatttgccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATagg                                                                                                                                                                                                              >  1:54768‑M2/191‑251 (MQ=255)                      attattggcatgacactgacaatttactttttaggaaaaggatttgccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATagg                                                                                                                                                                                                              <  1:224522‑M2/61‑1 (MQ=255)                         attggcatgacactgacaatttactttttaggaaaaggatttgccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATaggagg                                                                                                                                                                                                           >  1:138048‑M2/188‑251 (MQ=255)                          ttggcatgacactgacaatttactttttaggaaaaggatttgccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGc                                                                                                                                                                                                          <  1:37942‑M2/65‑1 (MQ=255)                                    actgacaatttactttttaggaaaaggatttgccgtaacatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAAc                                                                                                                                                                                                <  1:90798‑M2/75‑1 (MQ=255)                                                                    ccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGc                                                                                                                                                                                                          <  1:149953‑M2/65‑1 (MQ=255)                                                                    ccgtaatatctggcaaaattgaagctccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACaa                                                                                                                                                                >  1:277751‑M2/145‑251 (MQ=255)                                                                                              ccagcaacagcattaatttatgattttgtcacaaaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataatggatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGc                                                                                                                                                                                                          <  1:148022‑M2/65‑1 (MQ=255)                                                                                                                               aaaatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGATAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAAcg                                                                                                                   >  1:351355‑M2/86‑237 (MQ=255)                                                                                                                                 aatgattattgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAAt                                                                                                   >  1:523010‑M2/84‑251 (MQ=255)                                                                                                                                        attgtggcatttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCtattta                                                                                            >  1:826185‑M2/77‑251 (MQ=255)                                                                                                                                                 tttatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCT‑‑TAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGatt                                                                                 <  1:241258‑M2/184‑1 (MQ=255)                                                                                                                                                   tatgatgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGatt                                                                                 <  1:476571‑M2/186‑1 (MQ=255)                                                                                                                                                        tgaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGc                                                                            <  1:386138‑M2/191‑1 (MQ=255)                                                                                                                                                         gaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAAt                                                                                                                            <  1:91944‑M2/143‑1 (MQ=255)                                                                                                                                                         gaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCa                                                                           >  1:587990‑M2/60‑251 (MQ=255)                                                                                                                                                         gaattatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCa                                                                           >  1:609080‑M2/60‑251 (MQ=255)                                                                                                                                                           attatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAAc                                                                                                                                                                                     <  1:879531‑M2/86‑1 (MQ=255)                                                                                                                                                           attatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCa                                                                           >  1:228092‑M2/58‑249 (MQ=255)                                                                                                                                                           attatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCa                                                                           >  1:515339‑M2/58‑249 (MQ=255)                                                                                                                                                           attatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCAc                                                                          <  1:4241‑M2/193‑1 (MQ=255)                                                                                                                                                            ttatggggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGACACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCa                                                                           >  1:438952‑M2/57‑248 (MQ=255)                                                                                                                                                                  ggggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGc                                                                       <  1:885011‑M2/196‑1 (MQ=255)                                                                                                                                                                   gggtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGcc                                                                  <  1:848174‑M2/201‑1 (MQ=255)                                                                                                                                                                     gtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCtatttatt                                                                                          >  1:600198‑M2/48‑224 (MQ=255)                                                                                                                                                                     gtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCg                                                                 <  1:142546‑M2/202‑1 (MQ=255)                                                                                                                                                                     gtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTg                                                               >  1:238080‑M2/48‑251 (MQ=255)                                                                                                                                                                     gtatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTg                                                               >  1:100361‑M2/48‑251 (MQ=255)                                                                                                                                                                      tatctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGc                                                                            >  1:594226‑M2/47‑237 (MQ=255)                                                                                                                                                                         ctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAAc                                                                                                                                                                                          >  1:892916‑M2/44‑124 (MQ=255)                                                                                                                                                                         ctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCtatttatt                                                                                          <  1:803188‑M2/177‑1 (MQ=255)                                                                                                                                                                         ctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGt                                                           >  1:215261‑M2/44‑251 (MQ=255)                                                                                                                                                                         ctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGt                                                           >  1:204509‑M2/44‑251 (MQ=255)                                                                                                                                                                         ctagataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGt                                                           >  1:549450‑M2/44‑251 (MQ=255)                                                                                                                                                                           agataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGTAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGcc                                                                  <  1:98597‑M2/201‑1 (MQ=255)                                                                                                                                                                           agataactctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGttt                                                         >  1:136156‑M2/42‑251 (MQ=255)                                                                                                                                                                                actctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTggg                                                    <  1:281989‑M2/215‑1 (MQ=255)                                                                                                                                                                                 ctctttatcgataattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTg                                                                        >  1:477613‑M2/36‑230 (MQ=255)                                                                                                                                                                                       atcgataattgatgatcttaaaactagatAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAATACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTc                                              >  1:376033‑M2/30‑250 (MQ=255)                                                                                                                                                                                            taattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTAtttt                                                                                        >  1:798024‑M2/25‑203 (MQ=255)                                                                                                                                                                                             aattgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCAc                                                                          <  1:766993‑M2/193‑1 (MQ=255)                                                                                                                                                                                               ttgatgatcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGaa                                     >  1:300441‑M2/22‑251 (MQ=255)                                                                                                                                                                                                     atcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTAt                                                                                                                                                >  1:564690‑M2/16‑138 (MQ=255)                                                                                                                                                                                                      tcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGAc                                                                                                                                                                  <  1:279010‑M2/105‑1 (MQ=255)                                                                                                                                                                                                      tcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCtatttatt                                                                                          <  1:78263‑M2/177‑1 (MQ=255)                                                                                                                                                                                                      tcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAataaaagg                              >  1:727216‑M2/15‑249 (MQ=255)                                                                                                                                                                                                      tcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAg                              >  1:722471‑M2/15‑251 (MQ=255)                                                                                                                                                                                                      tcttaaaactagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAg                              >  1:498708‑M2/15‑251 (MQ=255)                                                                                                                                                                                                              ctagttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCa                      <  1:535426‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                agttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGAt                           <  1:313499‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                agttAAAACGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGc                       <  1:581924‑M2/244‑1 (MQ=255)                                                                                                                                                                                                                    aaaaCGCCGGTTTCTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCgg                 >  1:867499/1‑250 (MQ=255)                                                                                                                                                                                                                     aaacgccggtttcTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATAc                         <  1:624850/229‑1 (MQ=255)                                                                                                                                                                                                                      aacgccggtttcTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGcac              <  1:240279/240‑1 (MQ=255)                                                                                                                                                                                                                          ccggtttcTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTATTGAATAAAAAGGATACGCAACCGGCACAgg           <  1:73374/243‑1 (MQ=255)                                                                                                                                                                                                                             gtttcTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTaa       >  1:119866/5‑251 (MQ=255)                                                                                                                                                                                                                                tcTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCa                      <  1:629910/232‑1 (MQ=255)                                                                                                                                                                                                                                 cTTATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATaggag                                                                                                                                                                                                            >  1:82661/1‑50 (MQ=255)                                                                                                                                                                                                                                  ttATAACACAACCATTTCGCCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGaaa  >  1:460001/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  TTATGATGACGGTATTTTTACTTATCTTGGATTTGCCCCAAATAAAACCTTTCCTGCAGCCTTCTTGTTAGAAGGCTCCACGGAAAGTCTGTTAAATACTAATGTTAAACAAGACGGTAATTATCAGGTATTAGTCATCCAAAAAACAGCAGAAAAATTGGTATTACGTAGCGGTGAAAAAGTGGTCGGCATTTACAATCAAAGTTACGGAAAAACACCGGTAACTTATAACACGACCATTTCACCAAATGTGGCAAGAGACGAACGATAGGAGGCAGAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAA  >  1/1201524‑1201999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑