Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,199,192	C→T	intergenic (+39612/+221745)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,199,192	0	C	T	100.0%	173.5 / NA	65	intergenic (+39612/+221745)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/35);  total (30/35)

TATGAATACTCGAGGTGTTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTATTGGATTCAGTTAGTCAATCTATGAGTAGCAATTTTGCTAACTCACTTTTTACAATTATTGGCGTAACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAGATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGCCACAAAAATGATCATTGTAGCATTTATGATGAATTATGGAGGGTATCTGGATAACTCTTTAGCGATAATTGATGATCTCAAAACTAGCTTAACCGGCTTTACCGGAAACGGTATAGCCGGTTTAATGGATGACCAATTGGAATTAGGATCGGATATTGCTGGTCAACTCTTTGATCTAGATAAATCTAAATATGTC  >  1/1198953‑1199388                                                                                                                                                                                                                                                |                                                                                                                                                                                                     tATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGa                                                                                                                                                                                           >  1:874965/1‑251 (MQ=255) tATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGa                                                                                                                                                                                           >  1:679291/1‑251 (MQ=255)     aaTACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGAttat                                                                                                                                                                                       <  1:894302/251‑4 (MQ=255)       tACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                                                                                                                                     <  1:103477/251‑1 (MQ=255)       tACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                                                                                                                                     >  1:712516/1‑251 (MQ=255)                           cTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTAt                                                                                                                                                                 <  1:402433/251‑1 (MQ=255)                             aaTTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgg                                                                                                                                                               <  1:400285/251‑1 (MQ=255)                                 aGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggggg                                                                                                                                                           >  1:417582/1‑251 (MQ=255)                                  ggTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggg                                                                                                                                                             <  1:689464/248‑3 (MQ=255)                                         aTTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCtaga                                                                                                                                                   <  1:117896/251‑4 (MQ=255)                                          ttATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                                                                                                  <  1:142177/251‑1 (MQ=255)                                          ttATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                                                                                                  <  1:33248/251‑1 (MQ=255)                                            aTGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATaa                                                                                                                                                >  1:724366/1‑251 (MQ=255)                                             tGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                                                                                                                                               <  1:546754/251‑1 (MQ=255)                                             tGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                                                                                                                                               <  1:608627/251‑1 (MQ=255)                                                 aTTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                                                                                                             <  1:687334/249‑1 (MQ=255)                                                    cTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATggggg                                                                                                                                                            <  1:882512/231‑4 (MQ=255)                                                    cTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTat                                                                                                                                        <  1:868717/251‑2 (MQ=255)                                                            gTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                                                                                                  <  1:603979/233‑1 (MQ=255)                                                            gTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAAtt                                                                                                                                >  1:561047/1‑251 (MQ=255)                                                             tATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAAt                                                                                                                                 <  1:775996/249‑1 (MQ=255)                                                             tATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATtg                                                                                                                               >  1:383339/1‑251 (MQ=255)                                                                  aTTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCt                                                                                                                                                      <  1:899676/223‑1 (MQ=255)                                                                        tCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCtaga                                                                                                                                                   >  1:560229/1‑217 (MQ=255)                                                                        tCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTaaa                                                                                                                    >  1:223851/1‑251 (MQ=255)                                                                             aTTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                                                                                                                                               <  1:230326/219‑1 (MQ=255)                                                                                  gttactagATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTatgatg                                                                                                                                                                       <  1:797260/183‑1 (MQ=255)                                                                                  gttactagATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                                                                                                             <  1:213634/209‑1 (MQ=255)                                                                                     actagATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATtgatg                                                                                                                            >  1:484124/5‑230 (MQ=255)                                                                                                ttAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttc                                                                                               <  1:26449‑M1/248‑17 (MQ=255)                                                                                                   gTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAAt                                                                                                                                                                    >  1:315764/1‑176 (MQ=255)                                                                                                   gTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                                                                                                  <  1:486337/226‑1 (MQ=255)                                                                                                   gTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataa                                                                                         >  1:218607‑M1/1‑229 (MQ=255)                                                                                                       aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATa                                                                                                                                                 <  1:533840/191‑1 (MQ=255)                                                                                                       aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                                                                                                  <  1:605751/222‑1 (MQ=255)                                                                                                       aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacaca                                                                                     >  1:649167‑M1/1‑225 (MQ=255)                                                                                                        tCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgg                                                                                                                                                               >  1:135131/1‑176 (MQ=255)                                                                                                        tCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaa                                                                                    >  1:291681‑M1/1‑224 (MQ=255)                                                                                                           aTGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGTTTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                                                                                                             >  1:669837/1‑191 (MQ=255)                                                                                                           aTGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                                                                                                  <  1:75221/186‑1 (MQ=255)                                                                                                                     gttttGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                                                                                                             >  1:144544/2‑181 (MQ=255)                                                                                                                       tttgccagctcacttttTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatg                                                                     <  1:710751‑M1/243‑43 (MQ=255)                                                                                                                              gcTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAAt                                                                                                                                                                    >  1:10097/2‑149 (MQ=255)                                                                                                                              gcTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgg                                                                                                                                                               <  1:432333/153‑1 (MQ=255)                                                                                                                                 cACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCGTTTATGATGAATTATGGGGGGTAt                                                                                                                                                        >  1:686537/1‑158 (MQ=255)                                                                                                                                   cTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                                                                                                  >  1:151066/1‑194 (MQ=255)                                                                                                                                       ttACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                                                                                                  >  1:871753/1‑190 (MQ=255)                                                                                                                                          cAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggggg                                                                                                                                                           <  1:891894/146‑1 (MQ=255)                                                                                                                                          cAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATtgat                                                                                                                             >  1:236837/1‑176 (MQ=255)                                                                                                                                            attattGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgatagg                                                >  1:54768‑M1/1‑188 (MQ=255)                                                                                                                                            attattGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgatagg                                                <  1:224522‑M1/251‑64 (MQ=255)                                                                                                                                               attGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgataggagg                                             >  1:138048‑M1/1‑185 (MQ=255)                                                                                                                                                ttggcatgacactgacaattTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgataggaggc                                            <  1:37942‑M1/243‑68 (MQ=255)                                                                                                                                                   gcatgacacTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                                                                                                  >  1:836005/6‑178 (MQ=255)                                                                                                                                                       gacacTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGa                                                                                                                                     >  1:405555/2‑155 (MQ=255)                                                                                                                                                        acacTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                                                                                                             >  1:75380/1‑146 (MQ=255)                                                                                                                                                          acTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAACATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgataggaggcagagatgaac                                  <  1:90798‑M1/251‑78 (MQ=255)                                                                                                                                                             gACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                                                                                                  <  1:450193/168‑1 (MQ=255)                                                                                                                                                                             ggAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGAtt                                                                                                                                                                                         <  1:683966/81‑2 (MQ=255)                                                                                                                                                                                          ccGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                                                                                                                                     <  1:653225/72‑1 (MQ=255)                                                                                                                                                                                          ccGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                                                                                                  >  1:150265/1‑107 (MQ=255)                                                                                                                                                                                          ccGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTatc                                                                                                                                       >  1:569432/1‑116 (MQ=255)                                                                                                                                                                                          ccGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgataggaggcagagatgaacgataacaaaaccattcagccgacggatgacaa  >  1:277751‑M1/1‑142 (MQ=255)                                                                                                                                                                                          ccGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgataggaggc                                            <  1:149953‑M1/209‑68 (MQ=255)                                                                                                                                                                                                                    ccAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATGGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaaccatttcgccaaatgtggcaagagacgaacgataggaggc                                            <  1:148022‑M1/183‑68 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                     TATGAATACTCGAGGTGTTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTATTGGATTCAGTTAGTCAATCTATGAGTAGCAATTTTGCTAACTCACTTTTTACAATTATTGGCGTAACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAGATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGCCACAAAAATGATCATTGTAGCATTTATGATGAATTATGGAGGGTATCTGGATAACTCTTTAGCGATAATTGATGATCTCAAAACTAGCTTAACCGGCTTTACCGGAAACGGTATAGCCGGTTTAATGGATGACCAATTGGAATTAGGATCGGATATTGCTGGTCAACTCTTTGATCTAGATAAATCTAAATATGTC  >  1/1198953‑1199388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑