Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,199,076	T→C	intergenic (+39496/+221861)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,199,076	0	T	C	100.0%	208.0 / NA	72	intergenic (+39496/+221861)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/42);  total (30/42)

CTGAACGTCAAAAAATGTTAGAAGAATGTGAGAAAAAAGCCGAACCGCTGATGGCAAATTCTAATTGTCGAAACGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGTTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTATTGGATTCAGTTAGTCAATCTATGAGTAGCAATTTTGCTAACTCACTTTTTACAATTATTGGCGTAACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAGATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGCCACAAAAATGATCATTGTAGCATTTATGATGAATTATGGAGGGTATCTGGATAACTCTTTAGCGATAATTGATGATCTCAAAACTAGCTTAACCGGCTTTACCGGAAACGGTAT  >  1/1198850‑1199306                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       cTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGc                                                                                                                                                                                                                <  1:191853/251‑1 (MQ=255)       gTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAatta                                                                                                                                                                                                                     <  1:858491/240‑1 (MQ=255)             aaaTGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACa                                                                                                                                                                                                    <  1:78536/251‑1 (MQ=255)                   tAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTAc                                                                                                                                                                                              <  1:136938/251‑1 (MQ=255)                               agaaaaaagcggagccattaaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGaaaa                                                                                                                                                                                  >  1:407200/21‑251 (MQ=255)                                               attaaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAatat                                                                                                                                                                  <  1:6411/247‑1 (MQ=255)                                                ttaaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATc                                                                                                                                                                 <  1:382966/248‑1 (MQ=255)                                                  aaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTg                                                                                                                                                               <  1:476506/250‑1 (MQ=255)                                                           ctccaattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGAc                                                                                                                                                                                                     <  1:39186/188‑1 (MQ=255)                                                           ctccaattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTg                                                                                                                                                      >  1:159639/17‑251 (MQ=255)                                                              caattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAg                                                                                                                                                   >  1:112412/14‑251 (MQ=255)                                                              caattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAg                                                                                                                                                   >  1:175638/14‑251 (MQ=255)                                                                   gtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCa                                                                                                                                              >  1:660035/9‑251 (MQ=255)                                                                        aatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAAc                                                                                                                                         <  1:583683/248‑1 (MQ=255)                                                                          tgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAatat                                                                                                                                                                  >  1:830391/2‑224 (MQ=255)                                                                          tgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAg                                                                                                                                       >  1:23569/2‑251 (MQ=255)                                                                                tAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTaa                                                                                                                                 <  1:16334/251‑1 (MQ=255)                                                                                        aagcaagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAt                                                                                                                         <  1:641640/251‑1 (MQ=255)                                                                                          gcaagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAt                                                                                                                         <  1:233181/249‑1 (MQ=255)                                                                                           caagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAtttt                                                                                                                      >  1:78949/1‑251 (MQ=255)                                                                                           caagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAtttt                                                                                                                      >  1:225970/1‑251 (MQ=255)                                                                                           caagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAtttt                                                                                                                      >  1:375826/1‑251 (MQ=255)                                                                                              gTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGAt                                                                                                                                                                              <  1:455285/192‑1 (MQ=255)                                                                                                     aCGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCa                                                                                                                                           >  1:632170/1‑220 (MQ=255)                                                                                                        tATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGa                                                                                                         >  1:679291/1‑251 (MQ=255)                                                                                                        tATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGa                                                                                                         >  1:874965/1‑251 (MQ=255)                                                                                                            aaTACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGAttat                                                                                                     <  1:894302/251‑4 (MQ=255)                                                                                                              tACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                                                   <  1:103477/251‑1 (MQ=255)                                                                                                              tACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                                                   >  1:712516/1‑251 (MQ=255)                                                                                                                           aaTTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTGATTTATGAt                                                                                                                         >  1:476538/1‑216 (MQ=255)                                                                                                                                  cTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTAt                                                                               <  1:402433/251‑1 (MQ=255)                                                                                                                                    aaTTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgg                                                                             <  1:400285/251‑1 (MQ=255)                                                                                                                                        aGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggggg                                                                         >  1:417582/1‑251 (MQ=255)                                                                                                                                         ggTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggg                                                                           <  1:689464/248‑3 (MQ=255)                                                                                                                                             aTCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACttt                                                                                                                                                                                                                              <  1:286516/97‑1 (MQ=255)                                                                                                                                             aTCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTaa                                                                                                                                 <  1:894348/190‑1 (MQ=255)                                                                                                                                                aTTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCtaga                                                                 <  1:117896/251‑4 (MQ=255)                                                                                                                                                 ttATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                <  1:142177/251‑1 (MQ=255)                                                                                                                                                 ttATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                <  1:33248/251‑1 (MQ=255)                                                                                                                                                   aTGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATaa                                                              >  1:724366/1‑251 (MQ=255)                                                                                                                                                    tGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                                                             <  1:546754/251‑1 (MQ=255)                                                                                                                                                    tGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                                                             <  1:608627/251‑1 (MQ=255)                                                                                                                                                        aTTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                           <  1:687334/249‑1 (MQ=255)                                                                                                                                                           cTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATggggg                                                                          <  1:882512/231‑4 (MQ=255)                                                                                                                                                           cTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTat                                                      <  1:868717/251‑2 (MQ=255)                                                                                                                                                                   gTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                <  1:603979/233‑1 (MQ=255)                                                                                                                                                                   gTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAAtt                                              >  1:561047/1‑251 (MQ=255)                                                                                                                                                                    tATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAAt                                               <  1:775996/249‑1 (MQ=255)                                                                                                                                                                    tATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATtg                                             >  1:383339/1‑251 (MQ=255)                                                                                                                                                                         aTTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCt                                                                    <  1:899676/223‑1 (MQ=255)                                                                                                                                                                               tCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCtaga                                                                 >  1:560229/1‑217 (MQ=255)                                                                                                                                                                               tCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTaaa                                  >  1:223851/1‑251 (MQ=255)                                                                                                                                                                                cGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGa                                                                                                                          >  1:572285/1‑162 (MQ=255)                                                                                                                                                                                    aTTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                                                             <  1:230326/219‑1 (MQ=255)                                                                                                                                                                                         gttactagATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTatgatg                                                                                     <  1:797260/183‑1 (MQ=255)                                                                                                                                                                                         gttactagATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                           <  1:213634/209‑1 (MQ=255)                                                                                                                                                                                            actagATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATtgatg                                          >  1:484124/5‑230 (MQ=255)                                                                                                                                                                                                       ttAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttc             <  1:26449‑M1/248‑17 (MQ=255)                                                                                                                                                                                                          gTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAAt                                                                                  >  1:315764/1‑176 (MQ=255)                                                                                                                                                                                                          gTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                <  1:486337/226‑1 (MQ=255)                                                                                                                                                                                                          gTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataa       >  1:218607‑M1/1‑229 (MQ=255)                                                                                                                                                                                                              aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGc                                                                                                                                                             <  1:366225/97‑1 (MQ=255)                                                                                                                                                                                                              aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAAtt                                                                                                                               <  1:746554/127‑1 (MQ=255)                                                                                                                                                                                                              aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAAtt                                                                                                                               <  1:306409/127‑1 (MQ=255)                                                                                                                                                                                                              aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATa                                                               <  1:533840/191‑1 (MQ=255)                                                                                                                                                                                                              aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAAc                                <  1:605751/222‑1 (MQ=255)                                                                                                                                                                                                              aTCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacaca   >  1:649167‑M1/1‑225 (MQ=255)                                                                                                                                                                                                               tCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgg                                                                             >  1:135131/1‑176 (MQ=255)                                                                                                                                                                                                               tCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTAAAACTAGttaaaacgccggtttcttataacacaa  >  1:291681‑M1/1‑224 (MQ=255)                                                                                                                                                                                                                  aTGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGTTTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                           >  1:669837/1‑191 (MQ=255)                                                                                                                                                                                                                  aTGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                                                <  1:75221/186‑1 (MQ=255)                                                                                                                                                                                                                            gttttGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                                                           >  1:144544/2‑181 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       CTGAACGTCAAAAAATGTTAGAAGAATGTGAGAAAAAAGCCGAACCGCTGATGGCAAATTCTAATTGTCGAAACGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGTTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTATTGGATTCAGTTAGTCAATCTATGAGTAGCAATTTTGCTAACTCACTTTTTACAATTATTGGCGTAACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAGATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGCCACAAAAATGATCATTGTAGCATTTATGATGAATTATGGAGGGTATCTGGATAACTCTTTAGCGATAATTGATGATCTCAAAACTAGCTTAACCGGCTTTACCGGAAACGGTAT  >  1/1198850‑1199306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑